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Kit for rapidly inducing large number of DC-CIK and NK cells with lymphocyte culture medium and use method of kit

A technology of DC-CIK and lymphocytes, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve the problem of no induction kit

Inactive Publication Date: 2016-08-03
TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no simple, efficient and stable induction kit

Method used

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  • Kit for rapidly inducing large number of DC-CIK and NK cells with lymphocyte culture medium and use method of kit
  • Kit for rapidly inducing large number of DC-CIK and NK cells with lymphocyte culture medium and use method of kit
  • Kit for rapidly inducing large number of DC-CIK and NK cells with lymphocyte culture medium and use method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] like figure 1 , 2 , shown in 3, DC-CIK of the present invention, the test kit of NK cell, comprise DC cover box: 10ug dry powder DC-A, 5ug dry powder DC-B; CIK cover box: 6 dry powder CIK-A (30ug / support) , 10ug dry powder CIK-B, 20ug dry powder CIK-C; NK kit: 5 dry powder NK-A (50ug / piece), 10ug dry powder NK-B, 5ug dry powder NK-C, according to mass percentage, each component The ingredients are as follows:

[0074] DC box:

[0075] Dry powder DC-A: 40% interleukin-4 (4000IU / ug), 60% granulocyte-macrophage colony-stimulating factor (25000IU / ug);

[0076] Dry powder DC-B: 100% tumor necrosis factor-α (16000IU / ug);

[0077] CIK kit:

[0078] Dry powder CIK-A: 70% interleukin-2 (15000IU / ug), 30% amino acid;

[0079] Dry powder CIK-B: 70% CD3 monoclonal antibody, 30% interferon-gamma (28000IU / ug);

[0080] Dry powder CIK-C: 25% CD3 monoclonal antibody, 75% CD28 monoclonal antibody;

[0081] NK box:

[0082] Dry powder NK-A: 40% interleukin-2 (15000IU / ug), 30% in...

Embodiment 2

[0104] The DC-CIK and NK cell kits of the present invention include DC kits: 10ug dry powder DC-A, 5ug dry powder DC-B; CIK kits: 6 dry powder CIK-A (30ug / branch), 10ug dry powder CIK- B. 20ug dry powder CIK-C; NK kit: 5 dry powder NK-A (50ug / bottle), 10ug dry powder NK-B, 5ug dry powder NK-C, according to mass percentage, the composition of each component is as follows:

[0105] DC box:

[0106] Dry powder DC-A: 50% interleukin-4 (4000IU / ug), 50% granulocyte-macrophage colony-stimulating factor (25000IU / ug);

[0107] Dry powder DC-B: 100% tumor necrosis factor-α (16000IU / ug);

[0108] CIK kit:

[0109] Dry powder CIK-A: 75% interleukin-2 (15000IU / ug), 25% amino acid;

[0110] Dry powder CIK-B: 80% CD3 monoclonal antibody, 20% interferon-gamma (28000IU / ug);

[0111] Dry powder CIK-C: 30% CD3 monoclonal antibody, 70% CD28 monoclonal antibody;

[0112] NK box:

[0113] Dry powder NK-A: 40% interleukin-2 (15000IU / ug), 30% interleukin-12 (7000IU / ug), 30% interleukin-15 (7000...

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Abstract

The invention discloses a kit for rapidly inducing a large number of DC-CIK and NK cells with a lymphocyte culture medium and a use method of the kit. The kit comprises a DC set box: 10mu g of dry powder DC-A and 5mu g of dry powder DC-B, a CIK set box: 6 pieces of dry powder CIK-A (30mu g / piece), 10mu g of dry powder CIK-B and 20mu g of dry powder CIK-C, an NK set box: 5 pieces of dry powder NK-A (50mu g / piece), 10mu g of dry powder NK-B, and 5mu g of dry powder NK-C. By adopting the DC-CIK and NK cell induction kit, with the combination of the lymphocyte culture medium, lymphocyte more than or equal to 5*10<9> can be induced and amplified within the shortest time, the percentage of CIK cells (CD3<+>CD56<+>) is more than or equal to 25%, and the percentage of NK cells (CD3<->CD56<+>) is more than or equal to 50%. A convenient method is provided for in-vitro large-scale amplification of DC-CIK and NK cells, and convenience can be brought to related research and clinical testes.

Description

technical field [0001] The invention relates to a kit for inducing DC-CIK and NK cells and a use method thereof, in particular to a kit for rapidly inducing a large amount of DC-CIK and NK cells in combination with a lymphocyte culture medium and a use method thereof. Background technique [0002] Currently, immune cells have been gradually recognized by the medical community because of their high-efficiency tumor cell-killing activity and their advantages of no side effects. DC-CIK and NK (Cytokine induced killer) cells are the two most representative types of immune cells. The methods of induction and expansion are different, and the number and quality of induced cells are also uneven. There is no simple, efficient and stable induction kit. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a simple, efficient and stable kit for quickly inducing a large amount of DC-CIK and NK cells in combination with lymphocyte...

Claims

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Application Information

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IPC IPC(8): C12N5/0784C12N5/0783
CPCC12N5/0639C12N5/0638C12N5/0646C12N2500/32C12N2501/22C12N2501/2302C12N2501/2304C12N2501/2312C12N2501/2315C12N2501/25C12N2501/51C12N2501/515C12N2501/599C12N2506/115
Inventor 秦臻鲁振宇韩洪起张冰晶刘俊江徐悦
Owner TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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