Bacillus subtilis jtfm1001 and its application in the prevention and control of aflatoxin pollution in corn
A technology of JTFM1001 and Bacillus subtilis, applied in bacteria-containing organic fertilizers to prevent and control aflatoxin pollution in corn, in the field of Bacillus subtilis JTFM1001, it can solve the problems of easily polluted environment, fungicide resistance or even drug resistance, Chemical prevention and control of high cost issues, to achieve the effects of pollution prevention, stable antibacterial and detoxification effects, and good antibacterial and detoxification effects
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Embodiment 1
[0043] Example 1: Isolation and identification of strains with deposit number CCTCC NO:M 2016145
[0044]Healthy corn kernels from Hebei, Shandong, Shanxi, and Jilin provinces were selected, and the weighed corn kernels were soaked in a sterile triangular flask with 3% sodium hypochlorite solution for 10 minutes, and finally rinsed with sterile water for 5 times and drained. Clamp the corn kernels with tweezers, divide the kernels into 3 equal parts with sterile scissors, place them on the beef extract peptone medium plate, and cultivate them at a constant temperature of 28°C. Observe the growth of the colonies every day, and pick out the obvious differences in characteristics and separation in time. A good single colony was further purified to obtain a single strain, which was preserved on beef extract peptone slant medium for future use.
[0045] Morphological features:
[0046] Morphological observation found that the colony of strain JTFM1001 on LJTFM medium was round, wi...
Embodiment 2
[0053] Example 2: Experiment on the inhibitory effect of strains with preservation number CCTCC NO:M 2016145 on Aspergillus flavus in corn
[0054] Determination of biological activity of biocontrol strains such as Bacillus subtilis JTFM1001:
[0055] (1) The effect of Bacillus subtilis JTFM1001: The endophytic antagonistic bacteria of Aspergillus flavus were screened by the confrontation culture method. Place the pre-prepared Aspergillus flavus plate in the center of the slant medium plate, then use a high-temperature sterilized toothpick to pick up the endophytic bacteria, equidistant (2cm away from the pathogenic bacteria) symmetrical points on the slant medium plate, and then Incubate in the dark at 28°C, observe the presence and size of the inhibition zone for 3-7 days, and repeat 3 times. The endophytic bacterial strains with wider inhibition zone were selected for re-screening on peanut granules. Among the 350 strains tested, the Bacillus subtilis JTFM1001 strain has ...
Embodiment 3
[0067] Example 3: Preparation of microbial organic fertilizer by fermentation of strains with preservation number CCTCC NO: M 2016145 and its preparation method
[0068] The microbial organic fertilizer for preventing and controlling the contamination of corn by Aspergillus flavus in the field was prepared by fermenting the strain with the preservation number CCTCC NO: M 2016145. The content of the strain with the preservation number CCTCC NO: M 2016145 in the organic fertilizer was 1.5×10 8 Above cfu / g, organic matter content is 38-45%.
[0069] The industrialized production method of this microorganism organic fertilizer, the steps are as follows:
[0070] 1) Inoculate the strain with the preservation number CCTCC NO:M 2016145 into the liquid medium for fermentation production.
[0071] The liquid medium is: corn flour 3.3% (by weight ratio), peptone 1.45% (by weight ratio), K 2 HPO 4 +KH 2 PO 4 0.45% (by weight), water 1000ml, pH 7.2.
[0072] where K 2 HPO 4 and KH...
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