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Formaldehyde-free tissue specimen stationary liquid

A technology of tissue specimens and fixatives, applied in the field of pathology experiments, can solve the problems of poor preservation of nucleic acid molecules in tissue cells, shrinkage and hardening, etc., and achieve good fixation effects and complete preservation

Inactive Publication Date: 2016-08-10
北京九州柏林生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cross-linking reaction will cause shrinkage and hardening, and the antigen molecular structure, enzymes, and nucleic acid molecules of tissue cells are not well preserved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In this embodiment, the formaldehyde-free tissue specimen fixative consists of a mixed solution and additional components, wherein the mixed solution contains the following components in parts by volume: 45 mL of absolute ethanol, 20 mL of absolute methanol, 15 mL of polyethylene glycol-400, Purified water 10mL and fluorosurfactant 0.03mL, the added components include the following components, the final concentration of each component in the fixative solution is: anhydrous sodium carbonate 3g / L and trichloroacetic acid 6g / L .

[0024] Using the fixative of this embodiment, the fixation effect of the tissue cells is good, the cells are not deformed, and the cell morphology is preserved intact.

Embodiment 2

[0026] In this embodiment, the formaldehyde-free tissue specimen fixative consists of a mixed solution and additional components, wherein the mixed solution contains the following components in parts by volume: 50 mL of absolute ethanol, 25 mL of absolute methanol, 15 mL of polyethylene glycol-400, Purified water 20mL and fluorosurfactant 0.05mL, the added components include the following components, the final concentration of each component in the fixative solution is: anhydrous sodium carbonate 5g / L and trichloroacetic acid 8g / L .

[0027] Using the fixative of this embodiment, the fixation effect of the tissue cells is good, the cells are not deformed, and the cell morphology is preserved intact.

Embodiment 3

[0029] In this embodiment, the formaldehyde-free tissue specimen fixative consists of a mixed solution and additional components, wherein the mixed solution contains the following components in parts by volume: 45 mL of absolute ethanol, 25 mL of absolute methanol, 15 mL of polyethylene glycol-400, Polyethylene glycol-200 5mL, polyethylene glycol 600 5mL, polypropylene glycol-425 3mL, purified water 15mL and fluorosurfactant 0.03mL, the added components include the following components, and each component is fixed in the The final concentration in the liquid is: anhydrous sodium carbonate 3g / L and trichloroacetic acid 7.5g / L.

[0030] Using the fixative of this embodiment, the fixation effect of the tissue cells is good, the cells are not deformed, and the cell morphology is preserved intact.

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PUM

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Abstract

The invention discloses formaldehyde-free tissue specimen stationary liquid. The stationary liquid is composed of a mixed solution and additive components. The mixed solution is prepared from, by volume, 45-50 parts of anhydrous ethanol, 20-25 parts of absolute methanol, 15 parts of polyethylene glycol-400, 10-20 parts of purified water and 0.03-0.05 part of surfactant. The additive components include anhydrous sodium carbonate with the final concentration in the stationary liquid being 3-5 g / L and trichloroacetic acid with the final concentration in the stationary liquid being 6-8 g / L. The formaldehyde-free tissue specimen stationary liquid is prepared from non-toxic and harmless chemical reagents and is good in fixing effect, and shape of tissue cells is preserved completely.

Description

technical field [0001] The invention belongs to the technical field of pathological experiments, and relates to in vitro diagnostic reagents, in particular to a formaldehyde-free tissue specimen fixative. Background technique [0002] The birth of histopathology has a history of more than 150 years. In all hospitals, the pathological samples of surgical resection and various biopsy tissues are all made histopathological diagnosis on the surgical tissue samples by the hospital pathology department. [0003] Conventional paraffin-embedded HE staining is the most basic technique currently used to process tissue samples, it is the basis of pathological diagnostic methodology, and it is also the reference standard for evaluating the advantages and disadvantages of many new methods and technologies. The traditional method is to place the tissue sample in the tank of the treatment solution, pour or transfer in different treatment solutions in turn, and the sample is soaked in each ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 李明王德田
Owner 北京九州柏林生物科技有限公司
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