Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A simultaneous saccharification and fermentation process for cellulose-containing raw materials

A technology of simultaneous saccharification and fermentation, cellulose, applied in fermentation, biofuel and other directions, can solve the problems of low enzymatic hydrolysis efficiency of cellulose into ethanol, inability to achieve yeast/microorganism, unmatched fermentation temperature conditions, etc. Improve the efficiency of enzymolysis and fermentation, shorten the fermentation cycle, and shorten the effect of enzymolysis cycle

Active Publication Date: 2019-05-21
COFCO NUTRITION & HEALTH RES INST +2
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the mismatch between the cellulose enzymolysis temperature condition and the fermentation temperature condition in the synchronous saccharification and fermentation process of the prior art, the enzymolysis time is long, and the yeast / microbe suitable for ethanol liquid submerged fermentation cannot realize its normal and efficient metabolism The efficiency of enzymatic hydrolysis and the efficiency of converting cellulose into ethanol are not high due to activity, and a method of synchronously carrying out enzymolysis and submerged fermentation of cellulose-containing raw materials is provided, which improves the efficiency of enzymolysis and the conversion of cellulose into ethanol. Simultaneous saccharification and fermentation process of cellulosic feedstock with high ethanol efficiency and high ethanol yield

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A simultaneous saccharification and fermentation process for cellulose-containing raw materials
  • A simultaneous saccharification and fermentation process for cellulose-containing raw materials
  • A simultaneous saccharification and fermentation process for cellulose-containing raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] This example is used to illustrate the simultaneous saccharification and fermentation process of cellulose-containing raw materials of the present invention.

[0104] (1) Pretreatment of cellulose-containing raw materials

[0105] Cut 2,222 kg of impurity-free corn stalks (moisture content 10% by weight) into small sections not exceeding 1.2 cm x 0.5 cm x 1.0 cm, maintain a pressure of 1.0 MPa at 180°C for 5 minutes, and then release the pressure to complete steam explosion . A total of 4000 kg of steam explosion waste (water content 50% by weight) was obtained.

[0106] Determination of the total weight of cellulose and the total weight of hemicellulose in the resulting solid steam explosion product, see NREL / TP-510-42618 for details:

[0107]Take 10 grams of the above-mentioned steam explosion product and dry it at 45° C. to a constant weight of 5 grams, weigh 300.0 mg of the dried steam explosion product, and place it in a 100 ml sealable pressure-resistant glass t...

Embodiment 2

[0136] This example is used to illustrate the simultaneous saccharification and fermentation process of cellulose-containing raw materials of the present invention.

[0137] According to the method of Example 1, the cellulose-containing raw material was liquefied, and the liquefied product was subjected to simultaneous saccharification and fermentation, the difference was:

[0138] Before liquefaction, the pH value of the cellulose-containing raw material is adjusted to 4 with a sodium hydroxide solution having a pH regulator mass content of 40%.

[0139] In the liquefaction step (2), the temperature in the main enzymolysis device 2 is 40°C, and the added amount of the cellulose-containing raw material whose pH value has been adjusted is 320 kg / hour, expressed as the gram of fiber in the cellulose-containing raw material In terms of basic terms, the consumption of the cellulase is 10 enzyme activity units, and in the continuous liquefaction stage, the addition of the cellulase...

Embodiment 3

[0144] This example is used to illustrate the simultaneous saccharification and fermentation process of cellulose-containing raw materials of the present invention.

[0145] According to the method of Example 1, the cellulose-containing raw material was liquefied, and the liquefied product was subjected to simultaneous saccharification and fermentation, the difference was:

[0146] In the liquefaction step (2), cellulase and hemicellulase are continuously introduced into the tank body 21 from the enzyme inlet 27 at the top of the tank body 21 and the three supplementary enzyme inlets 29 at the side of the tank body 21, and are continuously introduced into the tank body 21 respectively. The amount of cellulase and hemicellulase added in the liquefaction space of the first layer, the second layer, the third layer and the fourth layer accounts for 30% and 30% of the total amount of cellulase and hemicellulase in the liquefaction stage respectively. 25%, 25%, and 20%. In the liqu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
angleaaaaaaaaaa
Login to View More

Abstract

The invention discloses a synchronous diastatic fermentation process of cellulose-containing raw materials, wherein the process comprises the following steps of mixing the cellulose-containing raw materials with enzyme; performing liquification under the liquification conditions so that soluble oligose in obtained liquification products is finally degraded to a state of 10 to 150g / L through being metered by the content of monosaccharide, the content of the monosaccharide is 10 to 100g / L, the viscosity of the liquification products is 200 to 500cp, and the average particle size of the liquification products is 20 to 100 [mu]m; mixing the liquification products with the enzyme and yeast; and performing synchronous diastatic fermentation. By using the technical scheme, the efficient synchronous diastatic fermentation process of the cellulose-containing raw materials is realized; the synchronous proceeding of the enzymolysis of the cellulose-containing raw materials and the liquid deep fermentation is realized, so that the enzymolysis period and the fermentation period are effectively shortened; the enzymolysis efficiency and the efficiency of converting cellulose into ethanol are further improved; and high ethanol yield is ensured.

Description

technical field [0001] The invention relates to a synchronous saccharification and fermentation process for cellulose-containing raw materials. Background technique [0002] Synchronous saccharification and fermentation process refers to the combination of enzymatic hydrolysis and ethanol fermentation in the same fermenter, and because the cellulose hydrolysis rate in the fermenter is much lower than the glucose consumption rate, it creates favorable conditions for converting sugar into ethanol condition. In addition, the advantages of the simultaneous saccharification and fermentation process include short fermentation time and reduced external microbial contamination. [0003] However, in the synchronous saccharification and fermentation process of the prior art, there are problems that the temperature conditions of cellulose enzymatic hydrolysis and fermentation temperature conditions do not match, the enzymatic hydrolysis time is long, and the yeast / microorganisms suita...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14
CPCY02E50/10
Inventor 岳国君武国庆熊强林鑫林海龙李凡沈乃东许克家高尚杜伟彦袁敬伟李春玲刘文信
Owner COFCO NUTRITION & HEALTH RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com