Method for screening kidney cancer peripheral blood miRNA marker and kidney cancer marker miR-378

A marker and kidney cancer technology, which is applied in the fields of genetic engineering and oncology, can solve the problems of patients suffering and inability to diagnose early, and achieve reliable diagnostic results and simple and fast clinical diagnosis

Inactive Publication Date: 2016-08-31
WUHAN TAIANKANG BIOTECH CO LTD
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Problems solved by technology

[0006] In order to solve the defects in the prior art that the diagnosis of kidney cancer still requires histological examination, which causes suffering to patients and cannot be diagnosed early, the present invention provides a method for screening serum / plasma miRNA markers of kidney cancer

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  • Method for screening kidney cancer peripheral blood miRNA marker and kidney cancer marker miR-378
  • Method for screening kidney cancer peripheral blood miRNA marker and kidney cancer marker miR-378
  • Method for screening kidney cancer peripheral blood miRNA marker and kidney cancer marker miR-378

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Embodiment 1

[0037] Example 1 screening renal cancer peripheral blood miRNA markers

[0038] 1. Summarize all circulating miRNAs that are associated with kidney cancer and have potential early diagnostic value

[0039] As of December 2015, all published literature related to renal cancer and involving circulating miRNAs were searched in the existing open database PubMed. The subject terms or keywords are as follows: "circulating" or "serum" or "plasma", "miRNA" or "microRNA" or "miR", "kidney" or "renal", and "cancer" or "carcinoma" or "neoplasm", combined with manual search methods to refer to meeting minutes or cited literature to obtain literature that meets the inclusion criteria . The inclusion criteria of the literature include: ①Patients were histopathologically confirmed RCC patients; ②Peripheral blood (including plasma and serum) used to detect miRNAs was collected before clinical treatment; ③The detection of circulating miRNAs and its relationship with kidney Research on the co...

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Abstract

The invention discloses a method for screening a kidney cancer peripheral blood miRNA marker and a miRNA marker. The screening method comprises the steps that miRNAs data related to the kidney cancer is summarized, meta analysis is performed, and one or more miRNAs with the best comprehensive analysis result are screened out to be adopted as candidate miRNAs; then, a TaqMan probe-based qRT-PCR method is adopted for perfomring crowd serum/plasma sample verification on the candidate miRNAs, and miRNAs with the expression threshold value of a patient suffering from the kidney cancer relative to healthy human serum/plasma being larger than 2.53 or smaller than 3.12; finally, the expression consistency of the miRNAs in kidney cancer tissue and serum/plasma is verified, and if it is verified that expression consistency is verified, the target marker is obtained. The invention further provides a kidney cancer serum/plasma miRNA marker miR-378. A novel approach is provided for noninvasive diagnosis of the kidney cancer, and the significant clinical practical value is achieved.

Description

technical field [0001] The invention relates to the fields of genetic engineering and oncology, in particular to a method for screening kidney cancer serum / plasma miRNA markers and the miRNA markers. Background technique [0002] Renal cancer is a common malignant tumor of the kidney originating from renal tubular epithelial cells, accounting for 2% to 3% of all malignant tumors in adults and 85% to 90% of primary malignant tumors in the kidney (Siegel R, Naishadham D, Jemal A Cancer statistics. CA Cancer J Clin, 2012, 62:10–29). There are many types of renal cell carcinoma. According to the 2004 WHO classification standard, renal cell carcinoma can be divided into clear cell carcinoma, papillary renal cell carcinoma, chromophobe cell carcinoma, collecting duct carcinoma and unclassified renal cell carcinoma pathologically. Clear cell carcinoma is predominant, accounting for about 60% to 85% (Mulders P, Figlin R, deKeon JB et al. Renal cell carcinoma: recent Progress and fu...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 徐华唐焜
Owner WUHAN TAIANKANG BIOTECH CO LTD
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