Cupric ion fluorescence enhancement type molecular probe and preparation method as well as application thereof

A technology of fluorescence enhancement and molecular probes, which is applied in the fields of fluorescence/phosphorescence, chemical instruments and methods, and material analysis through optical means, can solve the problems of low fluorescence quantum yield of chemical sensors, and achieve cheap and easy-to-obtain raw materials and production High rate, responsive effect

Active Publication Date: 2016-09-21
XUZHOU UNIV OF TECH
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  • Abstract
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  • Claims
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Problems solved by technology

However, due to the paramagnetism of copper, chemical sensors for copper ions generally have the d

Method used

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  • Cupric ion fluorescence enhancement type molecular probe and preparation method as well as application thereof
  • Cupric ion fluorescence enhancement type molecular probe and preparation method as well as application thereof
  • Cupric ion fluorescence enhancement type molecular probe and preparation method as well as application thereof

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Embodiment

[0043] A preparation method of copper ion fluorescence enhanced molecular probe, such as figure 1 Shown, its preparation method specifically carries out according to the following steps:

[0044] (1) Dissolve 10 g of urotropine dried at 35° C. for 4 hours under vacuum conditions in 37.5 mL of glacial acetic acid to obtain a mixed solution 1, and then dissolve 5.00 g of 4-methyl-7-hydroxycoumarin Mixed solution 2 is obtained in this mixed solution 1;

[0045] (2) Gradually heat up the mixed solution 2 to 120° C., react under reflux for 4 hours and then cool to room temperature;

[0046] (3) adding hydrochloric acid with a concentration of 2 mol / L dropwise into the reaction system obtained in step (2) until the pH of the reaction solution is about 4, and then stirring at 70° C. for 10 minutes;

[0047](4) Get step (3) gained reaction liquid 40mL, and wherein add the ice-water mixture of 40mL, then use the ethyl acetate of 40mL to extract in equal volume three times, combine th...

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Abstract

The invention discloses a cupric ion fluorescence enhancement type molecular probe and a preparation method as well as application thereof. The molecular formula of the probe is shown in the description; 4-methyl-7-hydroxy coumarin is taken as a fluorescence matrix, and reacts with hexamethylene second-amine; a formyl group is introduced at an ortho-position of hydroxy, and an obtained intermediate reacts with o-ethoxyphenol benzylamine; then nitrogen atoms and oxygen atoms are introduced; meanwhile the fluorescent light of a compound is enabled to close, and a specific reaction with cupric ions can be performed. By introducing the nitrogen atoms and the oxygen atoms for performing specific coordination with the cupric ions in the reaction matrix, a cupric ion sensor can quickly cooperate with the cupric ions in a detection process, so that fluorescent light is increased rapidly, thus realizing detection on the cupric ions and being capable of effectively avoiding the disturbance of other metal ions. The fluorescence probe prepared by the method is a high-selectivity fluorescence enhancement type cupric ion chemosensor simple in structure, cheap and easily available in material, easy to prepare and high in yield.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and specifically relates to a fluorescent molecular probe, in particular to a copper ion fluorescence-enhanced molecular probe, a preparation method thereof, and an application of the fluorescent molecular probe in identification and detection of heavy metal ions. Background technique [0002] The pollution of heavy metals to the environment and the harm to organisms are becoming more and more serious, which has aroused widespread concern. The detection of heavy metals in the environment and organisms is particularly important. Copper is a common heavy metal element widely present in the environment and organisms, and copper has become one of the important environmental pollutants. Due to the wide application of Cu in electroplating, construction and manufacturing industry, Cu 2+ Serious pollution to the environment. At the same time, copper ion is one of the indispensab...

Claims

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Application Information

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IPC IPC(8): C07D311/08C09K11/06G01N21/64
CPCC07D311/08C09K11/06C09K2211/1007C09K2211/1088G01N21/643
Inventor 田林堵锡华李昭盛风涛岳玮周颖梅郦志阳
Owner XUZHOU UNIV OF TECH
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