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S. pneumonia and capsular polysaccharide synthesis method, SPD-0064 gene coding product and purpose of coding product

A technology of Streptococcus pneumoniae and capsular polysaccharide, which is applied in the field of medicine and biology, can solve the problem of low production of capsular polysaccharide and achieve the effect of increasing production

Inactive Publication Date: 2016-09-21
遵义医学院第三附属医院
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Problems solved by technology

[0010] The present invention intends to provide Streptococcus pneumoniae to solve the problem of low capsular polysaccharide yield in the prior art

Method used

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  • S. pneumonia and capsular polysaccharide synthesis method, SPD-0064 gene coding product and purpose of coding product
  • S. pneumonia and capsular polysaccharide synthesis method, SPD-0064 gene coding product and purpose of coding product
  • S. pneumonia and capsular polysaccharide synthesis method, SPD-0064 gene coding product and purpose of coding product

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Embodiment Construction

[0036] The present invention is described in detail below by means of specific embodiments:

[0037]Streptococcus pneumoniae, including Streptococcus pneumoniae serotype strains, there is one or more capsular polysaccharide production inhibitory genes with overall deletion, partial deletion, sequence point mutation or sequence point deletion in the Streptococcus pneumoniae serotype strain; Membrane polysaccharide production inhibitor gene is the SPD-0064 gene or alleles of the SPD-0064 gene (different forms of the gene controlling a trait located at the same position on a pair of homologous chromosomes) or homologous genes (if the two gene sequences If the similarity reaches 80%, they can be called "homologous genes"), and the alleles of the SPD-0064 gene have more than 30% homology with the full-length or functional domain coding sequence of the SPD-0064 gene.

[0038] In this example, the selected capsular polysaccharide production inhibitory gene is the SPD-0064 gene, and t...

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Abstract

The invention discloses S. pneumonia in the technical field of bio-pharmacy. The S. pneumonia comprises an S. pneumonia serum type strain, wherein the S. pneumonia serum type strain includes one or a plurality of kinds of capsular polysaccharide generating inhabiting genes with integral lack, partial lack, sequence point mutation or sequence point lack. When the capsular polysaccharide generating inhabiting genes have one or a plurality of kinds of changes of partial lack, sequence point mutation or lack, the function and the activity of the SPD-0064 genes or coded products of the SPD-0064 genes can be influenced; the goal of interfering or inhibiting the combination of SPD-0064 gene coding products and capsular polysaccharide gene clusters is achieved, so that the transcription function of the capsular polysaccharide gene clusters is interfered or damaged; the capsular polysaccharide yield is finally increased.

Description

technical field [0001] The invention relates to the field of medical biotechnology, in particular to a synthesis method of Streptococcus pneumoniae and its capsular polysaccharide, an intermediate product of Streptococcus pneumoniae and the application of the intermediate product. Background technique [0002] Streptococcus pneumoniae (S.pn) is a Gram-positive diplococcus that looks like a spearhead and is arranged in pairs or short chains. There are capsules with a chemical composition of polysaccharides outside the virulent strains. The polysaccharide is called capsular polysaccharide (capsule, CPS). Capsular polysaccharide is a polysaccharide polymer commonly found on the surface of Gram-positive bacteria, which is fixed on the peptidoglycan of the outer layer of the cell wall by covalent bonding. At present, 98 kinds of capsular polysaccharides of Streptococcus pneumoniae have been identified; according to the antigenicity of capsular polysaccharides, Streptococcus pneum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31C12P19/04C12Q1/06C12Q1/14C12R1/46
CPCC07K14/3156C12P19/04C12Q1/06C12Q1/14
Inventor 吴凯峰尹一兵张雪梅徐红梅
Owner 遵义医学院第三附属医院
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