Method and device for determining concentration value based on visible light spectrum
A technology of visible light spectrum and concentration value, applied in the field of chemical analysis, it can solve the problems of limited analysis conditions, complex content determination, slow acquisition time, etc.
Inactive Publication Date: 2019-03-29
田发益
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[0008] The embodiment of the present invention provides a method and device for determining the concentration value based on the visible light spectrum, which is used to solve the problem in the prior art that the volume of the analytical instrument is relatively large and the conditions of use are relatively strict, which leads to complex content determination and is limited by the analysis conditions. The problem of slow acquisition time
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[0048] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0049] figure 2 A schematic flowchart of a method for determining a concentration value based on the visible light spectrum provided by an embodiment of the present invention, as shown in figure 2 As shown, a method for determining a concentration value based on the visible light spectrum provided by an embodiment of the present invention includes the following steps:
[0050] Step 101, performing a color reaction on the substance to be analyzed and the sta...
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The invention provides a method and device for determining a concentration value based on a visible light spectrum to solve the problems that existing content measuring is complex and the acquiring time is long and relates to the technical field of chemical analysis. The method comprises the steps that color development reaction is carried out on a substance to be determined and a solution to be determined, determination regions are selected from an image of a standard solution and an image of the solution to be determined, and statistics is carried out on light density values of the determination regions; reciprocals of light spectrum values of three primary colors in the light density values of the standard solution are taken and added together to be stored in a second document of the standard solution, regression fitting is carried out on numerical values in the second document of the standard solution, and a coefficient of the regression fitting equation is determined; a determination region is selected from the image of the solution to be determined, and statistics is carried out on the light density value of the determination region; reciprocals of light spectrum values of three primary colors in the determined light density values are taken and added together to be stored in a second document of the solution to be determined, the sum is substituted into the regression fitting equation, and the concentration value of the solution to be determined is determined.
Description
technical field [0001] The invention relates to the technical field of chemical analysis for detecting content by spectroscopy, in particular to a method and device for determining concentration values based on visible light spectrum. Background technique [0002] In the determination and analysis process of substance content, the determination of most substance content adopts spectrophotometry (including microplate reader). Methods for qualitative and quantitative analysis of the substance. [0003] In a spectrophotometer, when light of different wavelengths is irradiated continuously to a sample solution of a certain concentration, the absorption intensity corresponding to different wavelengths can be obtained. If the wavelength (λ) is taken as the abscissa and the absorption intensity (A) is taken as the ordinate, the absorption spectrum curve of the substance can be drawn. The method of qualitative and quantitative analysis of substances using this curve is called sp...
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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 田发益
Owner 田发益