Novel application of procyanidine in grape seeds

A technology of proanthocyanidins and anthocyanins, which is applied in the field of new application of proanthocyanidins in grape seeds, can solve the problems of cell apoptosis, influence on cancer cell infiltration and metastasis, and no report of radiation sensitization effect of lung cancer cells, so as to reduce pain and improve The effect of treatment efficiency and widening the range of application

Inactive Publication Date: 2016-10-12
LANZHOU UNIVERSITY
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Problems solved by technology

A small number of reports claim that GSPs can cause apoptosis, affect the invasion and metastasis of cancer cells, inhibit the expression of some enzymes, and have an inhibitory effect on the mitogen-activated protein kinase (MAPK) family (see literature: (1 ) PLoS One 2011, e21539. (2) Frontiers in Bioscience-landmark 2008, 13, 887–897. (3) Mol. Cancer Ther. 2010, 9, 569–580. (4) Carcinogenesis 2006, 27, 1682–1691. (5) Neoplasia 2005,7,24–36.(6)Clinical Cancer Research 2009,15,821–831.(7)Molecular Carcinogenesis 2009,48,232–42.), but there is no report on its radiation-sensitizing effect on lung cancer cells

Method used

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  • Novel application of procyanidine in grape seeds
  • Novel application of procyanidine in grape seeds
  • Novel application of procyanidine in grape seeds

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Experimental program
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Effect test

Embodiment

[0019] The GSPs used in the present invention are extracted from grape seeds with a purity of 73.3%. The extraction and purity determination process are as follows:

[0020] Extraction process: Weigh 500g of grape seeds and grind them, add 5L of ethanol at a material-to-liquid ratio of 1:10, and ultrasonically extract for 2h at 40°C, and concentrate the filtered solution under reduced pressure to obtain 58.4g of crude grape seed extract. Followed by macroporous resin column chromatography, with EtOH-H 2 O is the eluent, collect 50% fraction and concentrate under reduced pressure to obtain 5.3g crude proanthocyanidin sample.

[0021] Purity test:

[0022] Prepare different concentrations of proanthocyanidin standard solutions, measure the UV absorbance of each concentration at 546nm, and draw a standard curve, see figure 1 . The absorbance of grape seed extract was measured, and the content of grape seed proanthocyanidin was calculated according to the standard curve to be 73.3%, as ...

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Abstract

The invention discloses novel application of procyanidine in grape seeds, and application of procyanidine in preparation of a radiosensitizer. Possibility is provided to application of procyanidine in grape seeds, and a novel approach is provided to development of a novel radiosensitizer.

Description

Technical field [0001] The invention relates to a use of natural product extracts, in particular to a new use of proanthocyanidins in grape seeds. Background technique [0002] Procyanidins are widely found in many plants, and grape seed is a rich source of procyanidins. Grape seed proanthocyanidins (GSPs) have a wide range of uses, such as anti-oxidation, scavenging free radicals, UV radiation protection, etc., but there are few reports on their anti-tumor effects. A small number of reports have reported that GSPs can cause apoptosis, affect the infiltration and metastasis of cancer cells, inhibit the expression of some enzymes, and have inhibitory effects on the mitogen-activated protein kinase (MAPK) family (see reference: (1 )PLoS One 2011,e21539.(2)Frontiers in Bioscience-landmark 2008,13,887-897.(3)Mol.Cancer Ther.2010,9,569-580.(4)Carcinogenesis2006,27,1682-1691.(5)Neoplasia 2005, July, 24-36. (6) Clinical Cancer Research 2009, 15, 821-831. (7) Molecular Carcinogenesis 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/353A61P35/00
CPCA61K31/353A61K36/87
Inventor 李亚高坤孙萌焦兴志魏文君
Owner LANZHOU UNIVERSITY
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