9 results about "Grape seed proanthocyanidin" patented technology

The invention discloses antibacterial mouthwash as well as a preparation method and application thereof, and belongs to the technical field of daily chemical products. According to the invention, egg white lysozyme, ferulic acid and grape seed procyanidine are taken as core components, and the mouthwash with an efficient bacteriostatic effect is developed. And the antibacterial rates of streptococcus mutans and porphyromonas gingivalis are 96.88% and 96.30% respectively. Researches show that bacterial biofilms can be destroyed by using the mouthwash, and the biofilm quantity is reduced by 53.7% after the bacterial biofilms are treated for 24 hours; extracellular polysaccharide is inhibited, the content of water-insoluble glucan is reduced by 62.3%, and synthesis of a key cariogenic matrix is hindered; the acid production capacity is regulated and controlled, the pH change of 24-hour culture is only 1.50, and xylitol competitively inhibits glycolysis and cooperates with ferulic acid to block bacterial metabolism. The mouthwash prepared by the invention avoids the problem of enzyme activity inhibition of the ionic surfactant of the traditional mouthwash, takes effect more quickly, and provides a basis for developing natural, safe and efficient functional mouthwash.

The present application belongs to the technical field of livestock and poultry anti-virus micro-ecological preparation, and particularly relates to a bacillus compound preparation containing grape seed extract and application of the bacillus compound preparation in preparation of a medicine for treating or preventing PRRSV infection. Two strains of bacillus with significant inhibition of PRRSV infection and synergistic effect after being combined with grape seed extract are screened, and the preservation numbers are CCTCC NO: M2026187 and CCTCC NO: M2026186 respectively. The test results show that the compound preparation can significantly reduce the infection activity and proliferation capacity of PRRSV in cells and pigs, effectively reduce the clinical symptoms of pigs caused by PRRSV infection, and relieve the damage degree of lung tissue organs of pigs. The potential synergistic effect between the bacillus compound bacteria and grape seed proanthocyanidin provides a new technical scheme for effective prevention and control of porcine reproductive and respiratory syndrome.

This invention discloses a food composition with antioxidant and collagen synthesis-promoting effects and its preparation method, relating to the field of food technology. The food composition comprises the following ingredients in parts by weight: 300-500 mg collagen, 100-400 mg astragalus polysaccharide, 100-400 mg grape seed proanthocyanidins, 10-200 mg catechins, 5-100 mg ergothioneine, and 5-50 mg soybean oligopeptides. This food composition can increase the collagen content in the skin, enhance facial skin elasticity, reduce transepidermal water loss, and improve skin aging-related conditions. This invention constructs a dual-core synergistic formula of "antioxidant + collagen synthesis promotion" through the scientific formulation of seven active ingredients. All components of this invention use food-grade raw materials, making the formula mild and safe; all ingredients are natural extracts or edible raw materials, ensuring high safety, no obvious toxic side effects, and long-term daily use, making it suitable for a wide range of people.

The invention designs a new application of grape seed procyanidine in prevention and treatment of cataract. The method comprises the following steps: constructing a cataract injury model by inducing rabbit crystalline lens oxidative injury with hydrogen peroxide, culturing by adopting a domestic rabbit crystalline lens, detecting the activity of total superoxide dismutase, glutathione peroxidase and catalase and the content of malondialdehyde by observing the opacity and structural form of the crystalline lens, and detecting the expression of apoptosis regulation proteins BCL-2 and BAX in combination with immunohistochemistry. And the protective effect and molecular mechanism of the grape seed procyanidine on crystalline lens injury are researched. Researches show that the grape seed procyanidine has a protective effect on hydrogen peroxide induced rabbit crystalline lens injury, and the mechanism of the grape seed procyanidine is related to an antioxidant effect and adjustment of BCL-2 / BAX apoptotic protein; rabbit eye stimulation experiments prove that the grape seed procyanidine has no stimulation or only slight stimulation to rabbit eyes. Therefore, the grape seed procyanidine can prevent cataract, is a potential cataract treatment medicine, provides a new way for development of related medical products such as eye drops, and has good clinical application value.

The invention relates to application of autophagy in preparation of drugs or health food for improving fluorine-induced neurotoxicity from grape seed procyanidine. Rat and SH-SY5Y cell experiments are carried out, and the rat experiments prove that after intervention of different doses of GSPE, the SOD activity and the GSH content are improved, the MDA level is reduced, the antioxidant capacity of rats is recovered, autophagy is promoted, the filial generation learning and memory retention capacity is remarkably recovered, the autonomous activity capacity is recovered, anxiety behaviors are reduced, the occurrence and development processes of dental fluorosis are slowed down, and the occurrence and development of dental fluorosis are promoted. The change trends of different doses of GSPE present dose dependence, and the protection effect of a high-dose combined intervention group is most significant; in an SH-SY5Y cell experiment, the activity of the cells is improved through GSPE pretreatment, the expression quantity of cleave-caspase3 and cleaved-PARP is remarkably reduced, the autophagy process of the SH-SY5Y cells is promoted, and then the neurotoxicity caused by NaF is relieved. NaF is used as a representative, the prevention and treatment effects of GSPE on the fluorine-induced neurotoxicity are researched, and it is proved that GSPE can inhibit the NaF-induced neurotoxicity, so that the application of autophagy in preparation of drugs or health food for improving the fluorine-induced neurotoxicity from grape seed procyanidine is provided.

The invention belongs to the technical field of preparation of food, health care products and cosmetic additives, and relates to a grape seed procyanidine microemulsion and a preparation method thereof. The preparation method comprises the following steps: dissolving grape seed proanthocyanidins in a specific alcohol solution, and filtering to obtain a proanthocyanidins solution; respectively preparing a water phase consisting of inulin, trehalose and glycerol, and an oil phase consisting of sunflower phospholipid, MCT oil and a composite antioxidant; dropwise adding the water phase into the oil phase, and carrying out high-shear and high-pressure homogenization treatment to obtain a crude emulsion; and finally, injecting a procyanidine solution into the crude emulsion under the protection of inert gas, stirring in a dark place, carrying out rotary evaporation to remove alcohol residues, and filtering to obtain a final microemulsion product. According to the invention, by optimizing the proportion of the components, adopting the synergistic effect of the compound stabilizer and the antioxidant and strictly controlling the process conditions, the stability, the antioxidant activity and the bioavailability of the grape seed procyanidine are remarkably improved.

This invention discloses a heat-resistant microcapsule for targeted intestinal release of active substances, its preparation method, and its application, belonging to the field of food functional factor encapsulation technology. This invention uses grape seed proanthocyanidins obtained through single-stage alcohol extraction and macroporous resin purification as the core material, and high-methoxyl pectin (HMP) and hydroxypropyl methylcellulose phthalate (HPMCP) as composite wall materials. Microcapsules are prepared using a high-speed shear emulsification combined with spray drying process. Through the synergistic effect of the composite wall materials, this invention enables the microcapsules to simultaneously possess excellent heat-resistant processing stability and pH-responsive intestinal targeted release characteristics. It can significantly reduce the degradation and inactivation of grape seed proanthocyanidins during heat processing, storage, and gastric acid environments, efficiently preserving its proanthocyanidin content and its core low-glycemic function, primarily through the inhibition of α-amylase and α-glucosidase.

The application provides a lipid-lowering pharmaceutical composition and a preparation method thereof, and belongs to the technical field of medicines.The lipid-lowering pharmaceutical composition comprises the following components: 10-20 parts of spirulina extract, 5-15 parts of grape seed proanthocyanidin, 3-10 parts of conjugated linoleic acid, 2-8 parts of phytosterol and 1-5 parts of coenzyme Q10.Based on the lipid-lowering pharmaceutical composition, liposomes of spirulina extract and coenzyme Q10, microspheres of grape seed proanthocyanidin and phytosterol, and inclusion compounds of conjugated linoleic acid are prepared in sequence, and the liposomes, the microspheres and the inclusion compounds are mixed to obtain the lipid-lowering pharmaceutical composition.Through multi-level embedding and gradient release technology, the active ingredients can play roles in different parts of the body and at different time periods, the lipid-lowering effect is significantly improved, the side effect risk of high concentration exposure of single component is reduced, and the synergistic effect and precise release of the pharmaceutical composition are realized.

The invention provides a compound component gargle for preventing oral cancer of high-risk groups and a preparation method of the compound component gargle. The mouthwash is prepared from the following raw materials: 0.5 percent to 2.0 percent of epigallocatechin gallate, 0.3 percent to 1.5 percent of curcumin, 0.2 percent to 1.0 percent of epsilon-polylysine, 0.1 percent to 0.8 percent of vitamin B12, 0.5 percent to 3.0 percent of xylitol, 0.1 percent to 0.5 percent of menthol, 0.05 percent to 0.3 percent of rosemary extract, 0.03 percent to 0.2 percent of grape seed procyanidine, 0.1 percent to 0.6 percent of dipotassium glycyrrhizinate and the balance of purified water. The prepared gargle can effectively inhibit growth of microorganisms related to oral cancer, regulate oral micro-ecological balance and reduce the oral inflammation level, so that the risk of high-risk groups suffering from oral cancer is remarkably reduced.