A kind of extraction method of bitter melon saponin

A technology of bitter melon saponin and extraction method, which is applied in the field of extraction of bitter melon saponin, which can solve the problems of organic solvent residue, low yield of target product, and low active ingredient of medicine, and achieve the effects of low cost, increased yield, and mild reaction conditions

Active Publication Date: 2018-05-08
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a kind of extracting method of bitter melon saponin, increase the yield of total saponins of bitter melon through the combination of two-phase system and macroporous resin separation method, solve the problem of organic solvent residue in the existing extraction method, the active ingredient of the medicine is not high, and the target Problems such as low product yield

Method used

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  • A kind of extraction method of bitter melon saponin
  • A kind of extraction method of bitter melon saponin
  • A kind of extraction method of bitter melon saponin

Examples

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Embodiment 1

[0035] Example 1 was prepared using three different extraction methods

[0036] Bitter melon saponin S1 preparation of bitter melon saponin extract A: Take 55g of dried bitter melon powder and dissolve it in 70% ethanol at a solid-to-liquid ratio of 1:20 (g / mL), shake it well, and place it in a water bath at 60°C for 1h Then take it out, centrifuge at 4000rpm for 10min, combine the supernatant, obtain the bitter melon saponin alcohol extract, and vacuum concentrate in a rotary evaporator until the concentrated solution has no alcohol smell, and make the bitter melon saponin extract A;

[0037] S2 prepares bitter melon saponin extract B1: adopt ethanol / (NH 4 ) 2 SO 4 The aqueous two-phase system extracts bitter melon saponin alcohol extract A, the two-phase extraction conditions are that the tether length (TLL) is 45, and the ratio (R) is 2.5, wherein the mass fraction of each component is 45.195% ethanol, 33.594% ( NH 4 ) 2 SO 4 , after 30 minutes of equilibration, the p...

Embodiment 2

[0042] Example 2 In vitro hypoglycemic activity assay index evaluation

[0043] The samples in this example are finished products D1, D2, and D3 of acarbose and bitter melon saponin. Momordica saponin extracts A and B1 are purified and collected eluent with an ethanol concentration of 60-70%, and the ethanol is removed by rotary evaporation to prepare bitter melon saponin extracts C1 and C2 respectively, and Momordica saponin extract B2 is purified and collected ethanol The eluent with a concentration of 72-82% was rotatively evaporated to remove ethanol, and respectively prepared bitter melon saponin essence extract C3. Freeze-drying to prepare finished products of bitter melon saponins D1, D2 and D3 in the form of light yellow powder. The in vitro hypoglycemic activity of Momordica charantia saponin was evaluated by its inhibitory rate on α-glucosidase activity.

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Abstract

The invention relates to the technical field of natural product deep processing, in particular to a novel extraction method of momordica saponins. The novel extraction method comprises the following steps that 1, dry bitter gourd powder is taken to be dissolved into ethyl alcohol, oscillating and shaking are conducted to enable the mixture to be uniform, the mixture is put into a 60-DEG C water bath kettle to be extracted for 1 hour and then centrifuged to obtain a momordica saponins alcohol extracting solution, the momordica saponins alcohol extracting solution is subjected to vacuum concentration in a rotary evaporator until a concentrated solution is free of alcohol smell, and then a momordica saponins extracting solution A is prepared; 2, extraction is conducted by adopting an isopropyl alcohol-(NH4)2SO4 aqueous two-phase system, after partition equilibrium is achieved, phase separation is conducted, an upper phase is take, and a momordica saponins extracting solution B is prepared; 3, the crude momordica saponins extracting solution B is further purified through AB-8 type macroporous adsorption resin, and a fine momordica saponins extract C is prepared; 4, freeze drying is conducted to prepare the finished momordica saponins. Compared with a traditional method, the momordica saponins prepared through ethanol extraction, aqueous two-phase extraction and macroporous resin separation has the advantages that the yield of the momordica saponins is increased, and the blood glucose reducing activity of the momordica saponins is also obviously improved.

Description

technical field [0001] The invention relates to the technical field of deep processing of natural products, in particular to a method for extracting bitter melon saponin. Background technique [0002] Bitter gourd, belonging to the genus Momordica cucurbitaceae, is a natural plant that can be used for both medicine and food. Bitter gourd contains various chemical components such as bitter melon saponins, polysaccharides, proteins and polypeptides. Momordica saponin is a relatively complex glycoside compound, also known as glycoside (glycoside), which is a compound formed by linking sugar or sugar derivatives with another non-sugar substance through its terminal carbon atom. Saponins are composed of sugar, uronic acid or other organic acids. According to the chemical structure of aglycones, they can be divided into two categories: triterpene saponins and steroidal saponins. It is reported that it has functions such as anti-tumor, improving immunity, lowering blood sugar, inh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 刘杨李明莉肖湘
Owner SHANTOU UNIV
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