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A test paper for detecting alkaline xylanase activity and its preparation method

A technology for xylanase and xylan, which is applied to biochemical equipment and methods, measuring devices, and determination/inspection of microorganisms, can solve problems such as measurement difficulties, and achieves simple preparation process, easy operation, and good application prospects. Effect

Active Publication Date: 2018-10-09
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

However, its measurement is restricted by reagents, operation, experimental conditions, experimental accuracy, repeatability, etc. For many industries, it is difficult to measure

Method used

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  • A test paper for detecting alkaline xylanase activity and its preparation method

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Embodiment 1

[0026] Taking the alkaline xylanase produced by the fermentation of modified Trichoderma reesei ATCC56765 as an example, the crude enzyme activity of the xylanase is 80-100U / mL, and the optimum pH is 7.8;

[0027] (1) Prepare DNS chromogenic reagent, sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution with pH=7.80, and on this basis prepare alkaline xylan standard solution, xylanase standard solution and different concentration gradients Alkaline xylanase solution;

[0028] The preparation method of the DNS developer is as follows: liquid A: dissolve 6.90 g of crystalline phenol in 15.2 mL of 10% NaOH solution, dilute to 69 mL with distilled water, and add 6.90 g of sodium bisulfite to the solution. Solution B: Dissolve 255 g of potassium sodium tartrate in 300 mL of 10% NaOH solution, and then add 880 mL of 1% 3,5-dinitrosalicylic acid solution. Mix the two solutions of A and B to obtain the yellow reagent, which is stored in a brown bottle for 7-10 days ...

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Abstract

The invention discloses test paper for detecting activity of alkaline xylanase and a preparation method of the test paper. The preparation method comprises preparation steps as follows: a color developing agent and a buffer solution are prepared, and a standard xylanase solution and alkaline xylanase solutions with different gradients are prepared on the basis; the standard xylanase solution is applied to intermediate-speed qualitative filter paper, and test paper I is prepared; another base paper is immersed in the prepared color developing agent, soaked uniformly and aired, the surface layer of the base paper is coated with calcium oxide, and test paper II is prepared; the prepared test paper reacts and performs color developing in standard xylanase solutions with different gradients, a colorimetric card is prepared after photographing, and the activity of the detected xylanase sample can be obtained by comparing the color of the test paper with the colorimetric card. The preparation method adopts a simple preparation process and is easy to operate and suitable for industrial production; the test paper product can be effectively used for detecting the xylanase sample and has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of functional materials, and in particular relates to a test paper for detecting alkaline xylanase activity and a preparation method thereof. Background technique [0002] Test paper refers to a type of paper that has been impregnated with chemicals and can be used to test the presence of certain substances in liquids or gases through its color change. Various test strips have penetrated into various fields, and are widely recognized and accepted because of their convenience and low cost. [0003] Xylanases are hydrolases that degrade xylan into monosaccharides and oligosaccharides. For a long time, it has a relatively significant application value in papermaking, food, feed, energy and other industries, so it has attracted extensive attention of scientific researchers and has carried out related research on it. Enzyme activity is an important indicator to measure the biological activity of enzymes. For t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/34G01N21/78
CPCG01N21/78G01N2021/7759
Inventor 杨仁党马千里
Owner SOUTH CHINA UNIV OF TECH