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High speed micro scanning dynamic focusing device and focusing tracking method

A focusing device, high-speed technology, applied in microscopes, instruments, optics, etc., can solve the problems of time-consuming, unsuitable for high-speed microscopic scanning dynamic focusing, complex search process, etc., to achieve high reliability, improve focusing response speed and focusing The effect of tracking accuracy

Active Publication Date: 2016-10-12
上海谱华森生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0004] However, the above method needs to change the relative position between the sample and the objective lens many times during automatic focusing, the search process is complex and time-consuming, and it is not suitable for dynamic focusing of high-speed microscanning

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  • High speed micro scanning dynamic focusing device and focusing tracking method
  • High speed micro scanning dynamic focusing device and focusing tracking method

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Embodiment Construction

[0051] The present invention will be described in detail below in conjunction with specific embodiments. The following examples will help those skilled in the art to further understand the present invention, but do not limit the present invention in any form. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0052] This embodiment provides a dynamic focus device and a focus tracking method for high-speed micro-scanning;

[0053] The embedded high-speed focus tracking device is composed of an objective lens 102, a liquid lens focus detection module, an intelligent focus camera 109, a focus tracking execution module, and a focus control module 110;

[0054] The objective lens 102 is an infinity plan apochromatic objective lens of 20×, NA0.65, the field of view of the object side is about 1 mm×1 mm, and the de...

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Abstract

The invention provides a high speed micro scanning dynamic focusing device and a focusing tracking method. The device comprises an objective lens, a liquid lens defocus detection module, an intelligent focusing camera, a focusing tracking execution module and a focusing control module. The liquid lens defocus detection module is used for imaging an object space of different object space faces of a continuous scanning process on the target surface of the intelligent focusing camera. The intelligent focusing camera continuously acquires and processes a defocus detection image for defocus offset coding. The focusing tracking execution module drives the objective lens and a sample to carry out XY plane scanning motion and Z-axis focusing motion at the same time. The focusing control module controls the liquid lens defocus detection module to carry out continuous defocus state detection, and controls the focusing tracking execution module to carry out scanning motion and focusing motion coordination according to defocus offset coding of the intelligent focusing camera. The method provided by the invention comprises an initial focusing phase and a focusing tracking phase, which can greatly improve focusing response speed and focusing tracking precision.

Description

technical field [0001] The invention relates to the technical field of image acquisition, in particular to a dynamic focus device and a focus tracking method for high-speed micro-scanning. Background technique [0002] At present, the microscope focusing device mainly uses a high-speed focusing camera combined with a host computer for image acquisition and defocus information processing. The disadvantage is that there will be a large amount of time delay in image data transmission and host computer image processing; Non-real-time operating systems, and often need to take into account microscopic image acquisition and other communication and control functions, it is difficult to ensure the real-time performance of the entire focusing system, and cannot meet the focus tracking requirements in the high-speed microscopic scanning process. [0003] After searching: the patent number is: CN102062929B, and the name is: "An automatic focusing method and device for a microscope syste...

Claims

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Application Information

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IPC IPC(8): G02B21/24G02B21/36
CPCG02B21/241G02B21/361
Inventor 谷朝臣吴开杰关新平
Owner 上海谱华森生物科技有限公司
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