BGA position back bore manufacture method

A microscopic imaging and Bayesian technology, applied in the field of biological ultra-high-resolution microscopic imaging, can solve the problems of difficult to obtain dynamic changes of living cells, low positioning accuracy, and long computing time, so as to improve the running speed and space-time. High resolution, high positioning accuracy, and improved image continuity

Active Publication Date: 2016-10-12
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES +1
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Problems solved by technology

As a new important tool for cell imaging, although it can achieve ultra-high resolution imaging of the dynamic structure of living cells with simple optical equipment, there are still three key problems to be solved: 1) In terms of accuracy, there is a serious lack of structure, The positioning accuracy is not high; 2) In terms of speed, this method is extremely time-consuming. In order to obtain a super-resolution structure of 1.5 μm × 1.5 μm, it takes about 6 hours, and with picture As the size of the image increases, the calculation time increases sharply; 3) In terms of analysis scale, due to the limitation of speed, it is difficult for this method to obtain the large-scale and long-term dynamic changes of the entire living cell

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Embodiment Construction

[0026] The following is attached picture To describe the present invention in detail. It should be understood, however, that the picture are provided only for better understanding of the present invention, and they should not be construed as limiting the present invention.

[0027] In order to speed up the speed of high-density data reconstruction and improve the reconstruction accuracy, the present invention proposes a fast Bayesian microscopic imaging method (quick-3B), which includes two aspects: 1) the limited forward algorithm is used to Quickly calculate the observation probability of fluorescent molecules to speed up the analysis; 2) The cluster analysis method is used to reduce the influence of irrelevant fluorescent molecules and improve the reconstruction accuracy. The principles of the above two aspects are described in detail below.

[0028] 1) Limited forward algorithm

[0029] When analyzing fluorescence data using the 3B method, the initial number of fluoresc...

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Abstract

The invention discloses a BGA position back bore manufacture method. According to the invention, a back bore process is optimized; the problem of debris adhesion in the bore wall due to the high temperature during back bore processing is effectively avoided; a back bore is prevented from bad contact, short circuit and the like; the accuracy of back bore processing is improved; and the quality of a manufactured PCB is effectively improved.

Description

technical field [0001] The invention relates to a Bayesian microscopic imaging method, which belongs to the technical field of biological ultra-high resolution microscopic imaging. Background technique [0002] Ultra-high resolution fluorescence microscopy has a wide range of applications in the field of biological research, enabling people to better understand and understand cell biology. Like electron microscopy, ultra-high resolution fluorescence microscopy allows people to observe the internal structure and dynamic changes of cells that could not be observed before. Moreover, ultra-high resolution fluorescence microscopy also has incomparable advantages even electron microscopy can image live cells and can label multiple molecules with high specificity. [0003] Conventional optical microscopy cannot identify structures below 200 nm due to the limitation of optical diffraction. Ultra-high resolution fluorescence microscopy broke this limitation and won the Nobel Prize ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T5/50G06T3/40G06K9/62G01N21/64
CPCG06T3/4053G06T5/50G01N21/6458G06T2207/20076G06T2207/10061G06F18/24155
Inventor 徐平勇张法徐帆张名姝贺文婷
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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