An animal cell culture medium, a preparing method thereof and applications of the culture medium

A technology of animal cells and culture medium, applied in the field of cell culture medium, can solve the problems of high cost, unfavorable large-scale production and use, and inconvenient preparation, and achieve the effect of simple composition, easy control, and simple operation

Inactive Publication Date: 2016-10-19
中生天信和(无锡)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This medium is highly targeted and is only suitable for the cultivation of CHO series cells; in addition, it needs to synthesize specific

Method used

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  • An animal cell culture medium, a preparing method thereof and applications of the culture medium
  • An animal cell culture medium, a preparing method thereof and applications of the culture medium
  • An animal cell culture medium, a preparing method thereof and applications of the culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The animal cell culture medium of this embodiment is composed of the first component, the second component, the third component and the fourth component; wherein:

[0068] The first component consists of the following components by weight:

[0069]

[0070]

[0071] The second component consists of the following components by weight:

[0072]

[0073] The third component is made up of the composition of following parts by weight:

[0074]

[0075] The fourth component consists of the following components by weight:

[0076]

[0077] The animal cell culture medium can be prepared by the following method:

[0078] 1. Prepare the first component

[0079] Weigh each component in the first component according to the above weight ratio, mix the components evenly, and then place them in an electric blast drying oven for drying. The drying temperature is controlled at 80° C., and the drying time is 2.5 hours. Get the first component.

[0080] 2. Prepare the ...

Embodiment 2

[0091] The animal cell culture medium of this embodiment is composed of the first component, the second component, the third component and the fourth component; wherein:

[0092] The first component consists of the following components by weight:

[0093]

[0094]

[0095] The second component consists of the following components by weight:

[0096]

[0097] The third component is made up of the composition of following parts by weight:

[0098]

[0099]

[0100] The fourth component consists of the following components by weight:

[0101]

[0102] In the preparation method of the animal cell culture medium, the drying temperature is controlled to be 90°C and the drying time is 2 hours in the step of preparing the first component; the drying temperature is controlled to be 105°C and the drying time is 5 hours in the step of preparing the second component; In the step of preparing the third component, the total mass content of each component in the first mix...

Embodiment 3

[0104] The animal cell culture medium of this embodiment is composed of the first component, the second component, the third component and the fourth component; wherein:

[0105] The first component consists of the following components by weight:

[0106]

[0107] The second component consists of the following components by weight:

[0108]

[0109]

[0110] The third component is made up of the composition of following parts by weight:

[0111]

[0112] The fourth component consists of the following components by weight:

[0113]

[0114]

[0115] In the preparation method of the animal cell culture medium, the drying temperature is controlled to be 70°C and the drying time is 3 hours in the step of preparing the first component; the drying temperature is controlled to be 120°C and the drying time is within 3 hours in the step of preparing the second component. Except that all the other are the same as in Example 1, the average particle size of the prepared ...

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Abstract

An animal cell culture medium, a preparing method thereof and applications of the culture medium are provided. The culture medium comprises 150-230 parts by weight of amino acids or salts thereof, 180-280 parts by weight of carbohydrates, 160-260 parts by weight of inorganic salts, 1-2 parts by weight of vitamins and 0.002-0.003 part by weight of trace elements. The trace elements comprise manganese chloride tetrahydrate, sodium metavanadate, selenious acid, germanium dioxide, potassium bromide, chromium chloride hexahydrate, ammonium metavanadate, rubidium chloride, cadmium chloride, cobalt chloride hexahydrate, barium acetate, zirconyl chloride octahydrate, sodium fluoride, ammonium molybdate tetrahydrate, copper chloride dihydrate, lithium chloride and aluminium chloride hexahydrate. The culture medium is simple and definite in components, convenient to prepare and use, stable in quality, and low in batch differences, and can be particularly used for culturing a plurality of animal cells. Cell growth states are good and stable.

Description

technical field [0001] The invention relates to a cell culture medium, in particular to an animal cell culture medium and its preparation method and application. Background technique [0002] Cell culture medium is not only the basic material for supplying cell nutrition and promoting cell reproduction and proliferation in cultured cells, but also the living environment for the growth and reproduction of cultured cells. The composition of cell culture medium usually includes amino acids, vitamins, carbohydrates, inorganic ions, etc. Among them, amino acids are the basic units of protein, vitamins are biologically active substances that maintain cell growth, and play a role in regulating and controlling cell metabolism. It is the main energy source for cell growth. Inorganic ions are essential elements for cell composition and participate in cell metabolism. [0003] Cell culture media can be divided into four types: serum-containing media, serum-free media, protein-free med...

Claims

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Application Information

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IPC IPC(8): C12N5/07C12N5/071
Inventor 张宇王建超赵洪磊徐舸辰
Owner 中生天信和(无锡)生物科技有限公司
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