Juncao-based agrocybe aegirit culture medium and preparation method of agrocybe aegirit liquid strain

A technology of liquid strains and tea tree mushrooms, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of prolonging the movement route of bubbles, less mycelium, and large damage to strains, and achieve good results. Nutrients and living environment, improved stability, and easy operation

Inactive Publication Date: 2016-10-26
HEFEI FUQUAN MODERN AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrating power is not strong. If the compost is too thick, mycelium is not easy to penetrate the compost, and the mycelia will follow the compost. The surface grows rapidly, and the amount of mycelium inside the compost is very small, which seriously affects the yield and quality of the strain; 2. Although the conventional liquid strain solves some problems existing in the solid strain, it can significantly shorten the cultivation cycle and enhance the growth rate of the strain. Species vigor and stress resistance, reducing the contamination rate of strains, and improving the uniformity of strain quality, but there are still some deficiencies such as the density, size, and uniformity of the bacterial balls in the liquid strains obtained by fermentation are not very ideal. If the bacteria balls are crushed by stirring and shearing, the uniformity of the obtained strains will be more ideal. However, the shear force of high-speed stirring, crushing and beating will greatly damage the strains and have a se...

Method used

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Embodiment Construction

[0014] A tea tree mushroom culture medium based on Juncao, comprising the following components in parts by weight: Juncao 120, carrot powder 70, yeast autodissolving powder 90, royal jelly 100, potassium dihydrogen phosphate 0.4, magnesium sulfate 0.3, gypsum Powder 0.3, Sophorolipid 1, Calcium Propionate 1.2, Plant Hydrogenated Oil 0.2, Sodium Hydroxymethyl Cellulose 0.2, Cellulase 2, Light Calcium Carbonate 8, Nano Tourmaline Powder 5.6, Bacterial Nanocellulose 5, Appropriate amount of water.

[0015] A kind of method adopting above-mentioned culture medium to prepare tea tree mushroom liquid strain, comprises the following steps:

[0016] (1) Mix half of nano tourmaline powder and water at 0.1g / L, electrify to prepare sterile water for later use; mix the total amount of plant hydrogenated oil, sodium hydroxymethyl cellulose and sterile water at 1g / L Stir and dissolve at 30°C to obtain solution 1; mix and dissolve the total amount of potassium dihydrogen phosphate, magnesiu...

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Abstract

The invention discloses a juncao-based agrocybe aegirit culture medium which comprises the following components in parts by weight: 120-122 parts of juncao, 70-80 parts of carrot powder, 90-92 parts of yeast autolysis powder, 100-102 parts of royal jelly, 1-2 parts of sophorolipid, 1.2-1.4 parts of calcium propionate, 0.2-0.3 part of hydrogenated plant oil, 5.6-6 parts of nano-level tourmaline powder, 5-6 parts of bacterial nano-cellulose, and the like. According to the invention, the juncao subjected to enzymolysis, carrot powder, royal jelly and yeast autolysis powder are used as main materials to prepare an agrocybe aegirit liquid culture medium, thereby providing rich nutrients for agrocybe aegirit stock culture and expanding culture; in the preparation process of the liquid culture medium, by virtue of a sterilization effect of electrization of the nano-level tourmaline powder, auxiliary sterilization of the liquid culture medium is realized; compared with traditional sterilization, the operation is simple and the energy consumption is little; and meanwhile, by adding nano-tourmaline powder, nano-cellulose nutrients and carrier substances, the stability of mycelium pellet distribution can be increased, and a high-quality agrocybe aegirit liquid strain can be obtained.

Description

technical field [0001] The invention relates to the technical field of liquid strains, in particular to a Juncao-based tea tree mushroom culture medium and a method for preparing tea tree mushroom liquid spawns. Background technique [0002] In the production process of tea tree mushroom, liquid strains have incomparable advantages over solid strains. Liquid strains have short production cycle, short and consistent bacterial age, high purity, strong vitality, simple and fast inoculation, and easy implementation of industrialization and scale. , standardized production and other advantages. Now, more and more people are researching and using liquid strains, and the promotion and use of liquid strains is also an inevitable trend to realize industrialization, scale and standardization of edible fungi. However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrating power is not strong. I...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 柯家厚
Owner HEFEI FUQUAN MODERN AGRI SCI & TECH
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