Juncao-based agrocybe aegirit culture medium and preparation method of agrocybe aegirit liquid strain

Abstract

The invention discloses a juncao-based agrocybe aegirit culture medium which comprises the following components in parts by weight: 120-122 parts of juncao, 70-80 parts of carrot powder, 90-92 parts of yeast autolysis powder, 100-102 parts of royal jelly, 1-2 parts of sophorolipid, 1.2-1.4 parts of calcium propionate, 0.2-0.3 part of hydrogenated plant oil, 5.6-6 parts of nano-level tourmaline powder, 5-6 parts of bacterial nano-cellulose, and the like. According to the invention, the juncao subjected to enzymolysis, carrot powder, royal jelly and yeast autolysis powder are used as main materials to prepare an agrocybe aegirit liquid culture medium, thereby providing rich nutrients for agrocybe aegirit stock culture and expanding culture; in the preparation process of the liquid culture medium, by virtue of a sterilization effect of electrization of the nano-level tourmaline powder, auxiliary sterilization of the liquid culture medium is realized; compared with traditional sterilization, the operation is simple and the energy consumption is little; and meanwhile, by adding nano-tourmaline powder, nano-cellulose nutrients and carrier substances, the stability of mycelium pellet distribution can be increased, and a high-quality agrocybe aegirit liquid strain can be obtained.

Description

technical field [0001] The invention relates to the technical field of liquid strains, in particular to a Juncao-based tea tree mushroom culture medium and a method for preparing tea tree mushroom liquid spawns. Background technique [0002] In the production process of tea tree mushroom, liquid strains have incomparable advantages over solid strains. Liquid strains have short production cycle, short and consistent bacterial age, high purity, strong vitality, simple and fast inoculation, and easy implementation of industrialization and scale. , standardized production and other advantages. Now, more and more people are researching and using liquid strains, and the promotion and use of liquid strains is also an inevitable trend to realize industrialization, scale and standardization of edible fungi. However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrating power is not strong. I...

AI Technical Summary

Problems solved by technology

However, liquid strains also have their own defects and deficiencies: 1. The germination point of liquid strains usually grows quickly, but the penetrating power is not strong. If the compost is too thick, mycelium is not easy to penetrate the compost, and the mycelia will follow the compost. The surface grows rapidly, and the amount of mycelium inside the compost is very small, which seriously affects the yield and quality of the strain; 2. Although the conventional liquid strain solves some problems existing in the solid strain, it can significantly shorten the cultivation cycle and enhance the growth rate of the strain. Species vigor and stress resistance, reducing the contamination rate of strains, and improving the uniformity of strain quality, but there are still some deficiencies such as the density, size, and uniformity of the bacterial balls in the liquid strains obtained by fermentation are not very ideal. If the bacteria balls are crushed by stirring and shearing, the uniformity of the obtained strains will be more ideal. However, the shear force of high-speed stirring, crushing and beating will greatly damage the strains and have a serious impact on the overall quality of the strains.

Publication No. CN105309194A "New Technology for the Production of Edible Mushroom Microparticle Liquid Strain" provides a kind of edible fungus microparticle liquid strain with a mycelium ball particle diameter of 0.5mm, which increases the ventilation rate of the edible fungus liquid strain culture solution, so that the ventilation rate It is suitable at 1:0.6—0.9V / V·min. At the same time, a stirrer is installed in the aerated fermenter to make the eddy current of the culture liquid move and prolong the technical operation method of the air bubble. The method for producing the microparticle liquid strain is from the production process There is no improvement in the aspect of liquid culture medium, and the implementation is technically difficult and demanding; Publication No. CN105130516A "Method for Making Molecular-level Biological Medium" mentions that the nutrients of molecular-level biological medium can be absorbed quickly and comprehensively. Promotes the growth of liquid strains, but does not mention the effect of molecular-grade biological media on the diameter of liquid strain mycelium balls