A strain of pyridine-degrading dye-phagocytic bacteria and its application in the treatment of pyridine-containing wastewater
A wastewater treatment and pyridine technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria and other directions, can solve the problems of limited number of pyridine degrading strains, low pyridine tolerance concentration, single strain species, etc. The effect of high efficiency pyridine degradation ability, good application prospect and high mineralization ability
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Embodiment 1
[0015] Screening, isolation and identification of Pigmentiphaga sp.NJUST35.
[0016] (1) Screening and isolation of strains
[0017] The mature aerobic granular sludge was taken out from the existing SBR reactor used to treat pyridine wastewater, thoroughly ground with a mortar, and then diluted to 10 with sterile water using a gradient dilution method. 4 -10 10 times. Prepare LB solid medium, pour it into the same sterilized 90×90cm petri dish while it is hot after sterilization, apply 20 μL of the previously diluted sample after cooling and solidification, and place it in a biochemical incubator at 30°C Culture 3d. The strains with obvious differences in colony characteristics on the petri dish were selected and purified by the method of plate streak separation. After three consecutive purifications, a single strain was obtained and stored on a slant. Pick the isolated single colonies, insert them into the inorganic salt medium MSM containing pyridine as the only carbon ...
Embodiment 2
[0026] Study on the degradation performance of Pigmentiphaga sp.NJUST35 strain on pyridine.
[0027] Inoculate the pyridine-degrading strain NJUST35 into the LB medium supplemented with 1000 mg / L pyridine, and culture it on a shaker at a speed of 180 r / min at 30°C to carry out the enrichment culture of NJUST35 until the bacteria enter the late logarithmic phase (about 3 days ), the obtained thalline was centrifuged at a speed of 8000rpm for 15 minutes, the supernatant was skimmed off, and the thalline was resuspended in sterile liquid MSM by vortex shaking, and centrifuged. After repeating the washing process three times, the bacteria were resuspended in sterile liquid MSM (adjust the amount of MSM added to control the OD of the bacteria suspension) 600 about 1.0) to obtain the seed solution. The LB medium used in the present invention is a conventionally used medium in the technical field.
[0028] Prepare liquid MSM containing 1000mg / L pyridine as simulated wastewater, add...
Embodiment 3
[0031] Treatment of pyridine-containing industrial wastewater by Pigmentiphaga sp.NJUST35.
[0032] Put the Pigmentiphaga sp.NJUST35 seed solution into the actual industrial wastewater containing pyridine (containing 989mg / L of pyridine, COD1328mg / L, 55mg / L of total phosphorus) with an inoculum amount of 5%, at a speed of 180r / min at 30°C Shaker culture. Monitor the COD value, pyridine and ammonia nitrogen concentration before and after wastewater treatment.
[0033] It can be seen from Table 1 that after the pyridine-containing industrial wastewater was treated by the strain Pigmentiphaga sp.NJUST35 for 108 hours, the pyridine was completely degraded, the COD value was reduced to 314.67mg / L, and the ammonia nitrogen release was 63.11mg / L. The pyridine degradation rate, COD removal rate and ammonia nitrogen conversion rate were 100%, 76.3% and 35.61%, respectively.
[0034] This example shows that the isolated Pigmentiphaga sp.NJUST35 can be successfully applied to the bioch...
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