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Evaluation method of Gossypium hirsutum-Gossypium barbadense introgression lines

An introgression system, land-sea technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as difficult to fine-tune positioning and large workload.

Active Publication Date: 2016-10-26
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

This method has a large workload and is limited by the size of the mapping population and the number of molecular markers, making it difficult to fine-map the genetic position of the disease resistance gene / QTL
In the existing research, there are few reports on the evaluation of Verticillium wilt resistance of a single land-sea introgressive material

Method used

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  • Evaluation method of Gossypium hirsutum-Gossypium barbadense introgression lines

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Embodiment Construction

[0020] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0021] (1) Origin of land-ocean introgression system

[0022] The upland cotton variety Zhongmiansuo 36 was crossed with Haidao Mianhai 1 to obtain F1. The BC5F1 generation was obtained by backcrossing 5 generations with the recurrent parent Zhongmian 36. Then, BC5F3 land-sea introgression line population was obtained by selfing for three generations. A stable land-ocean introgression line 2047 was selected from this population for this study.

[0023] (2) Cotton verticillium wilt resistance identification

[0024] In the greenhouse, the identification of the resistance of single strains was carried out by quantitatively dipping the vermiculite sand paper pot into the bacterial solution. The seeds of Luhai introgression line, Zhongmian Institute 36, Jimian 11 (susceptible c...

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Abstract

The invention relates to an evaluation method of Gossypium hirsutum-Gossypium barbadense introgression lines. The invention discloses a comprehensive evaluation method of Gossypium hirsutum-Gossypium barbadense introgression lines. The method comprises the following steps: (1) identifying verticillium wilt resistance; (2) carrying out molecular marker analysis on Gossypium hirsutum-Gossypium barbadense introgression lines; (3) determining the physical location of the molecular marker in the cotton genome; and (4) according to the physical location of the molecular marker, carrying out physical location on the introduced disease-resistant chromosome segment, and drawing a physical map of the Gossypium hirsutum-Gossypium barbadense introgression lines. By combining the verticillium wilt resistance identification and molecular marker analysis, the molecular marker sequence is compared with the tetraploid Gossypium hirsutum genome, and the disease-resistant gene carried in the Gossypium hirsutum-Gossypium barbadense introgression lines is located, thereby laying the foundation for disease-resistant gene cloning and disease-resistant variety improvement.

Description

technical field [0001] The invention belongs to the field of crop breeding, in particular to molecular assisted breeding. In particular, it relates to a molecular marker analysis and Verticillium wilt resistance identification results to locate the sea-island cotton chromosome segment that is introduced into the upland cotton and carries the disease resistance gene. Background technique [0002] Cotton verticillium wilt (Verticillium wilt) is a soil-borne disease that infects vascular tissue caused by Verticillium dahliae Klebahn. Cancer” poses a serious threat to the cotton planting industry in major cotton-producing countries and regions in the world. In recent years, with the impact of global climate change and changes in farming systems, the disease has gradually spread in cotton-producing areas around the world, especially in high-temperature and rainy areas and years, which has attracted the attention of researchers all over the world. [0003] Upland cotton is the m...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2531/113
Inventor 陈婷婷袁有禄李鹏涛石玉真王艳玲张雷刘爱英龚举武商海红李俊文巩万奎葛群
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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