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Pancreas cancer genetic susceptibility gene detection kit

A technology for detecting kits and susceptibility genes, applied in the fields of molecular biology and medicine, can solve problems such as increased risk of pancreatic cancer, and achieve the effect of risk assessment

Inactive Publication Date: 2016-10-26
SICHUAN KINGMED DIAGNOSTICS CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that those who carry the 3Rc / 3Rc genotype have a significantly increased risk of pancreatic cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The method for using the pancreatic cancer genetic susceptibility gene detection kit comprises the following steps:

[0018] (1) Extraction of DNA template

[0019] Genomic DNA of oral epithelial cells was extracted using the silica gel adsorption method.

[0020] (2): Electrophoresis detection typing

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PUM

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Abstract

The invention discloses a pancreas cancer genetic susceptibility gene detection kit. The kit comprises a primer for detecting the Arg194Trp SNP site of X-ray repair cross-complementing gene 1XRCC1 and the SNP site of thymidine thymidine synthetase gene TS, and a specific fluorescence probe pair. The kit realizes assessment detection of the pancreas cancer genetic susceptibility risk through detecting the polymorphisms of the Arg194Trp SNP site of the X-ray repair cross-complementing gene 1XRCC1 and the SNP site of the thymidine thymidine synthetase gene TS, and has high detection sensitivity and accuracy.

Description

technical field [0001] The invention relates to the fields of molecular biology and medicine, in particular to a detection kit for pancreatic cancer genetic susceptibility genes. Background technique [0002] Pancreatic cancer has a high degree of malignancy, is difficult to detect early, metastasizes early and quickly, and has a poor prognosis. According to statistics, only 12% to 15% of cases can undergo radical surgery after diagnosis, and the 5-year survival rate after operation is only 0-2%, while more than 90% of patients die within 1 year after diagnosis, and the average survival period is less than 6 years. months. In the past, it was often said that liver cancer was the "king of cancers", while pancreatic cancer was the "king of kings". There are no obvious symptoms in the early stage of pancreatic cancer, or only upper abdominal discomfort and loss of appetite. Symptoms begin to appear after the cancer develops to a certain extent, including epigastric pain, jau...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/118C12Q2600/156
Inventor 周炜欧阳小峰毛家丽周宇
Owner SICHUAN KINGMED DIAGNOSTICS CENT