Method for identifying germination-stage composite salt-alkali resistance of semi-wild cotton

Abstract

The invention relates to plant stress-resisting physiology, in particular to a method for identifying germination-stage composite salt-alkali resistance of semi-wild cotton. The method comprises the following steps: 1), subjecting semi-wild cotton seeds to concentrated sulfuric acid delinting and then sterilizing; 2) preparing a composite salt-alkali culture substrate; 3) sowing the seeds; 4) evaluating resistance level of the semi-wild cotton material through statistics and result analysis. The method enables semi-wild cotton materials with high salt-alkali resistance to be quickly and accurately screened, the resistance is stable, the materials can endure single salt stress and can better adapt to complicated and diverse practical stresses, and therefore the method has higher practical utilization value and significance.

Description

technical field [0001] The invention relates to plant stress resistance physiology, in particular to a compound saline-alkali resistance identification method in the germination stage of semi-wild cotton. Background technique [0002] Semi-wild cotton (semi-wild cotton) is a wild species of upland cotton that has not been domesticated. It is produced in the Mexican highlands and the Caribbean in Central America, and has a rich source of stress resistance genes. [0003] Nowadays, most of the research on cotton's salt resistance focuses on a single salt (NaCl). However, in the natural environment, a variety of salts often exist in combination, and salts and alkalis are mixed and associated, which results in the results of single salt research. The expected results cannot be received, and practical problems cannot be solved. There is a large area of ​​saline-alkali soil in Xinjiang, my country, and has a complex saline-alkali environment, which is characterized by many saline...

AI Technical Summary

Problems solved by technology

[0004] At present, the salt-alkaline tolerance screening method of cotton cultivars is mainly the identification method of emergence rate in the germination stage. This method is a field screening method and is mainly aimed at cultivated cotton species. Some materials in semi-wild cotton materials have hard seed shells and low natural germination rate. The phenomenon

At the same time, the field identification method has shortcomings such as uneven distribution of field salt, great influence by the environment, and long identification period (about 45 days), resulting in poor repeatability of experimental results.

Moreover, only the germination rate is used as the evaluation standard, which is too single, and the result is greatly affected by the subjectivity.

Images