Method for identifying germination-stage composite salt-alkali resistance of semi-wild cotton

A technology for identification of semi-wild cotton and resistance, which is applied in the field of compound salt-alkali resistance identification of semi-wild cotton in the germination period, can solve the problems of uneven distribution of salt in the field, poor repeatability of experimental results, and low natural germination rate, and is easy to popularize. And the effect of utilization, short identification cycle, and avoidance of bacterial interference

Active Publication Date: 2016-11-09
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0004] At present, the salt-alkaline tolerance screening method of cotton cultivars is mainly the identification method of emergence rate in the germination stage. This method is a field screening method and is mainly aimed at cultivated cotton species. Some materials in semi-wild cotton materials have hard seed shells and low natural germination rate. The phenomenon
At the same ti

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  • Method for identifying germination-stage composite salt-alkali resistance of semi-wild cotton
  • Method for identifying germination-stage composite salt-alkali resistance of semi-wild cotton
  • Method for identifying germination-stage composite salt-alkali resistance of semi-wild cotton

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Embodiment 1

[0025] Embodiment 1 adopts the semi-wild cotton germination stage compound saline-alkali resistance identification method to semi-wild cotton (broad-leaved cotton 40, broad-leaved cotton 27, pointed spot cotton 25, broad-leaved cotton 32, broad-leaved cotton 120, Mary Galante Cotton 85) and cultivated cotton (Zhong 12, Zhong 16, E Jing No. 1, 7124) were identified and evaluated for their salt-alkali tolerance.

[0026] 1. Cotton material:

[0027] Semi-wild cotton (40 broad-leaved cotton, 27 broad-leaved cotton, 25 sharp-spotted cotton, 32 broad-leaved cotton, 120 broad-leaved cotton, 85 Mary Galante cotton), upland cotton (medium 12, medium 16, Ejing No. 1 ), sea-island cotton (7124) was the reference material for cultivated cotton.

[0028] 2. Pretreatment of seeds:

[0029] Sulfuric acid delints all the seeds with short fluff, selects the same size, black plump, complete mature seeds without pests, and then soaks them in 5% sodium hypochlorite solution for 10 minutes, was...

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Abstract

The invention relates to plant stress-resisting physiology, in particular to a method for identifying germination-stage composite salt-alkali resistance of semi-wild cotton. The method comprises the following steps: 1), subjecting semi-wild cotton seeds to concentrated sulfuric acid delinting and then sterilizing; 2) preparing a composite salt-alkali culture substrate; 3) sowing the seeds; 4) evaluating resistance level of the semi-wild cotton material through statistics and result analysis. The method enables semi-wild cotton materials with high salt-alkali resistance to be quickly and accurately screened, the resistance is stable, the materials can endure single salt stress and can better adapt to complicated and diverse practical stresses, and therefore the method has higher practical utilization value and significance.

Description

technical field [0001] The invention relates to plant stress resistance physiology, in particular to a compound saline-alkali resistance identification method in the germination stage of semi-wild cotton. Background technique [0002] Semi-wild cotton (semi-wild cotton) is a wild species of upland cotton that has not been domesticated. It is produced in the Mexican highlands and the Caribbean in Central America, and has a rich source of stress resistance genes. [0003] Nowadays, most of the research on cotton's salt resistance focuses on a single salt (NaCl). However, in the natural environment, a variety of salts often exist in combination, and salts and alkalis are mixed and associated, which results in the results of single salt research. The expected results cannot be received, and practical problems cannot be solved. There is a large area of ​​saline-alkali soil in Xinjiang, my country, and has a complex saline-alkali environment, which is characterized by many saline...

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Application Information

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IPC IPC(8): A01G7/00A01C1/02
CPCA01C1/02A01G7/00
Inventor 韦洋洋李振庆许艳超周忠丽刘方蔡小彦王星星王玉红王春英张振梅王坤波
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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