Method for increasing distortion rate of giant clam larvae
A technology of larvae and metamorphosis, applied in climate change adaptation, fish farming, application, etc., can solve the problems of inability to realize large-scale production of giant clam seedlings, low metamorphosis rate, etc., and achieve the goal of increasing larval metamorphosis rate and reliable technical guarantee Effect
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Embodiment 1
[0018] a. Controlling the density of larvae: On May 5, 2016, at the Hainan Tropical Marine Biology Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, the larvae of Tridacna pedescens were used as experimental materials, and they were placed in eight 10L containers filled with fresh seawater. Inside the plastic box, the bottom area of the plastic box is 25×20=500cm 2 , the density is adjusted to 5 / cm 2 (50,000 pieces / m 2 ), a total of 2,500 larvae were put in; during this period, the seawater used required double sand filtration, the temperature was controlled at 28-30°C, the salinity was controlled at 32-35ppt, and the pH was controlled at 8.1-8.3;
[0019] b. Add shell-shaped coralline algae: Shell-shaped coralline algae are collected from coastal coral reefs in Xiaodonghai, Sanya, Hainan, and then made into 1-2cm with hammers, pliers, etc. 2 After soaking in fresh sea water for 24 hours, add 5cm 2 Shell coralline algae, the...
Embodiment 2
[0024] a. Controlling the density of larvae: On May 10, 2016, at the Hainan Tropical Marine Biology Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, the larvae of Tridacna pedunculata were used as experimental materials and placed in six 10L containers filled with fresh seawater. Inside the plastic box, the bottom area of the plastic box is 25×20=500cm 2 , the density is adjusted to 10 / cm 2 (100,000 pieces / m 2 ), a total of 5,000 larvae were placed; during this period, the seawater used was filtered with a 2μm membrane, the temperature was controlled at 28-30°C, the salinity was controlled at 30-33ppt, and the pH was controlled at 8.1-8.3;
[0025] b. Add shell-shaped coralline algae: Shell-shaped coralline algae are collected from coastal coral reefs in Xiaodonghai, Sanya, Hainan, and then made into 1-2cm with hammers, pliers, etc. 2 After soaking in fresh sea water for 24 hours, add 4cm 2 Shell coral algae, scale 80cm 2 ...
Embodiment 3
[0030] a. Controlling the density of larvae: On May 9, 2016, at the Hainan Tropical Marine Biological Experimental Station of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, the larvae of the giant oyster's foot plate were used as experimental materials and placed in nine 10L plastic containers filled with fresh seawater. Inside the box, the bottom area of the plastic box is 25×20=500cm 2 , the density is adjusted to 5 / cm 2 (50,000 pieces / m 2 ), a total of 2,500 larvae were placed; during this period, the seawater used was filtered with a 2μm membrane, the temperature was controlled at 28-30°C, the salinity was controlled at 32-35ppt, and the pH was controlled at 8.0-8.3;
[0031] b. Add shell-shaped coralline algae: Shell-shaped coralline algae are collected from coastal coral reefs in Sanya, Hainan, and then made into 1-2cm pieces with hammers and pliers 2 After soaking in fresh sea water for 24 hours, add 5cm 2 Shell coralline algae, scale 100...
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