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Compound Cynanchum chinense extract and preparation method thereof

A technology of goose down vine and extract, applied in the field of biomedicine, can solve problems such as rare chemical composition research

Active Publication Date: 2016-11-09
苏州微敏检测技术服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

White cauliflower seed is the seed of white cauliflower (Cleome gynandra L.), which has the functions of expelling wind and cold, promoting blood circulation and relieving pain [Lu Bangyu et al., Research Progress of White Cauliflower, Modern Agricultural Science and Technology, 2014, (3): 74 ], has a synergistic effect with velvet rattan, but the research on its chemical constituents is relatively rare

Method used

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  • Compound Cynanchum chinense extract and preparation method thereof
  • Compound Cynanchum chinense extract and preparation method thereof
  • Compound Cynanchum chinense extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Extraction and separation of chemical components of white cauliflower seeds

[0013] The cauliflower seeds mainly contain flavonoids, alkaloids and other components, which have anti-inflammatory, analgesic and antibacterial effects, but there are no reports on the determination of the main chemical components and their content. In the invention, firstly, the main components in the white cauliflower seeds are subjected to liquid phase analysis, separation and purification.

[0014] White cauliflower seeds (batch number BZ140301, purchased from Wuhan City, Hubei Province) 800g medicinal material, reflux extraction 2 times with 5 times the amount of 70% ethanol solution, 2 hours each time, combine the two extracts, filter, and concentrate the filtrate to about 2L under reduced pressure. Extract twice with 2L of petroleum ether, remove the aqueous solution of the lower layer, put it on the pretreated AB-8 macroporous resin column, wash 4L with water first, and...

Embodiment 2

[0015] Embodiment 2: the preparation of compound goose down vine extract

[0016] The preparation of the compound velvet vine extract: Weigh 5 kg of velvet vine stem leaves (batch number ER130901, collected from the suburbs of Shihezi, Xinjiang Uygur Autonomous Region) and 10 kg of white cauliflower medicinal material respectively, mix them, heat and reflux extract twice with 75% ethanol 100L, and extract Combined, concentrated under reduced pressure to about 50L, left to stand overnight, filtered, and the filtrate was loaded on an AB-8 macroporous resin column, eluted with 100L of water and 50L of 70% ethanol in turn, collected the 70% ethanol eluate, concentrated under reduced pressure, Vacuum-dried at 60 degrees to obtain the compound velvet vine extract.

Embodiment 3

[0017] Embodiment 3: the content determination of compound goose down vine extract

[0018] Medicinal material test solution: Prepared according to the corresponding process of the compound goose down vine extract. Accurately weigh 9.94g of the velvet rattan medicinal material and 1148g of the white cauliflower medicinal material respectively, extract twice with 100ml of 75% ethanol under reflux, combine the extracts, concentrate under reduced pressure to about 40ml, and load the sample on the AB-8 macroporous resin column. Sequentially elute with 200ml of water and 100ml of 70% ethanol, collect the 70% ethanol eluate, concentrate it under reduced pressure to about 25ml, transfer it to a 50ml volumetric flask, and dilute with methanol to obtain a stock solution; accurately measure 2ml of the stock solution To a 25ml volumetric flask, dilute to volume with methanol to obtain the corresponding test solution of velvet rattan and white cauliflower seeds.

[0019] Reference substa...

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Abstract

The invention discloses a compound Cynanchum chinense extract and a preparation method thereof. The preparation method comprises the steps of: (1) according to a compound Cynanchum chinense formula, selecting corresponding mass of Cynanchum chinense and Cleome gynandra seeds (in a mass ratio of 10:3-10:30), using water or an ethanol-water mixed solvent to perform extraction and concentration; using macroporous resin to adsorb the obtained compound Cynanchum chinense extracted solution, then conducting washing, and performing elution with a 40-95% ethanol-water mixed solvent, concentrating and drying the eluent, thus obtaining the extract. The compound Cynanchum chinense extract contains not less than 20mg / g of tricin-7-O-glucuronide, also contains not less than 2.0mg / g of apigenin. The preparation process route of the compound Cynanchum chinense extract is economical and reasonable, high in efficiency, and is easy to realize industrialized production.

Description

technical field [0001] The invention relates to a compound velvet rattan extract, and also relates to a preparation method of the extract, which belongs to the field of biomedicine. technical background [0002] Compound velvet vine is a folk prescription developed by the inventor, which is composed of velvet vine, cauliflower seeds and other medicinal materials, and is used to treat various peripheral arthritic pains such as frozen shoulder and lumbar muscle strain. In this prescription, Cynanchum chinense R.Br. is the aboveground stem and leaf of Cynanchum chinense R.Br., and the inventor found that the extract of Cynanchum chinense R.Br. has certain anti-inflammatory activity in the previous research, and the main active ingredient is wheat Flavonoid components such as flavin-7-O-glucuronide, kaempferol-3-O-glucopyranoside, wheat flavin, and established its preparation method (Liu Jiangyun et al., a kind of velvet vine extract and its Preparation method, patent number ZL...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/27A61P19/02A61P29/00A61P21/00
CPCA61K36/185A61K36/27A61K2236/331A61K2236/333A61K2236/55A61K2300/00
Inventor 刘江云王增尚王川陈虹
Owner 苏州微敏检测技术服务有限公司
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