Method of removing phenylalanine in rice proteins

A technology of rice protein and phenylalanine, applied in the direction of food science, etc., can solve the problems of cumbersome steps, long time-consuming, low economic benefits, etc., and achieve the effect of simple operation and mild conditions

Active Publication Date: 2016-11-16
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the research on the removal of phenylalanine by combining enzymatic hydrolysis and adsorption is very important. The existing methods for removing phenylalanine are time-consuming, complicated steps and low economic benefits.

Method used

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  • Method of removing phenylalanine in rice proteins
  • Method of removing phenylalanine in rice proteins
  • Method of removing phenylalanine in rice proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The method for removing phenylalanine in rice protein comprises the following steps:

[0040] The first step: fully dissolve the rice protein pretreatment;

[0041]The second step: After the first step, the rice protein is fully dissolved, and chymotrypsin (addition amount 7200U / g protein) is added, and the enzymolysis is carried out in a constant temperature water bath shaker at 55°C for 6 hours;

[0042] Step 3: After the reaction in the second step, add pronase (98U / g protein) and mix evenly, and carry out enzymatic hydrolysis in a constant temperature water bath shaker at 45°C for 3 hours;

[0043] Step 4: Take it out after the reaction in the third step, put it in a constant temperature water bath at 90°C for 10 minutes to inactivate the protease, centrifuge at 4200r / min for 20 minutes, and take the supernatant to obtain the enzymatic hydrolyzate of rice protein;

[0044] Step 5: After the reaction in the fourth step, use D101 macroporous adsorption resin to adsor...

experiment example 1

[0054] Experimental example 1 Effect of different enzymatic hydrolysis times of chymotrypsin on the free effect of phenylalanine

[0055] The first step: fully dissolve the rice protein pretreatment;

[0056] The second step: After the first step, the rice protein is fully dissolved, and chymotrypsin is added (7200U / g protein), and the enzymolysis is carried out in a constant temperature water bath shaker at 55°C (4h / 6h / 8h / 10h / 12h);

[0057] Step 3: After the reaction in the second step, take it out, put it in a constant temperature water bath at 90°C for 10 minutes to inactivate the protease, centrifuge at 4200r / min for 20 minutes, and take the supernatant to obtain the enzymatic hydrolyzate of rice protein;

[0058] Step 4: After the reaction in the third step is completed, the solution is a rice protein solution in which phenylalanine is fully free, and the solution is preserved by freeze-drying.

[0059] The results of the effect of different enzymatic hydrolysis times of...

experiment example 2

[0060] Influence of experimental example 2 chymotrypsin adding amount on the free effect of phenylalanine

[0061] The first step: fully dissolve the rice protein pretreatment;

[0062] The second step: After the first step, the rice protein is fully dissolved, and chymotrypsin (addition amount 4800-14400U / g protein) is added, and the enzymolysis is carried out in a constant temperature water bath shaker at 55°C for 6 hours;

[0063] Step 3: After the reaction in the second step, take it out, put it in a constant temperature water bath at 90°C for 10 minutes to inactivate the protease, centrifuge at 4200r / min for 20 minutes, and take the supernatant to obtain the enzymatic hydrolyzate of rice protein;

[0064] Step 4: After the reaction in the third step is completed, the solution is a rice protein solution in which phenylalanine is fully free, and the solution is preserved by freeze-drying.

[0065] The results of the effect of chymotrypsin addition on the free effect of phe...

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Abstract

The present invention discloses a method of removing phenylalanine in rice proteins. The method comprises the following steps: the rice proteins are pre-treated and the pre-treated rice proteins are fully dissolved; and chymotrypsin and pronase are used to conduct a step-by-step enzymolysis of the rice proteins, and D101 macroporous adsorption resin adsorbs free phenylalanine in enzymolysis liquid to remove the phenylalanine in the rice proteins. A large amount of objective experiments are carried out to conduct multi-factor optimizations of conditions of enzymolysis and adsorption, and finally the method of removing the phenylalanine in the rice proteins is determined. The method is rapid and high-efficient, simple in operations and mild in conditions. By applying the method to conduct treatment of the rice proteins, the phenylalanine in the rice proteins decreases from 4.72% to 0.29%.

Description

technical field [0001] The invention relates to a method for removing phenylalanine in rice protein. Background technique [0002] Phenylketonuria (PKU) or phenylalanine hydroxylase deficiency (phenylalaninehydroxylase deficiency) is an autosomal recessive genetic disease. It is permanent damage to the central nervous system due to the inability of the body to properly metabolize phenylalanine. As long as the diagnosis is made in the neonatal period and a timely low-phenylalanine diet is given, the intellectual impairment of the children can be avoided. Diet therapy is a well-recognized method for treating phenylketonuria with significant long-term effects, that is, a method to control the intake of phenylalanine in children's food to achieve the metabolic balance of the body. [0003] At present, low-phenylalanine products mainly include enzymatic desorption and adsorption methods, compounding methods, and genetic methods. But the root is to efficiently remove phenylalan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
Inventor 刘红芝王强盛晓静石爱民刘丽胡晖杨颖
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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