A plasma miRNA marker related to auxiliary diagnosis of Hashimoto's thyroiditis and its application

A technology for Hashimoto's thyroiditis and auxiliary diagnosis, applied in the field of genetic engineering, can solve the problems of rarely reported diagnostic effects, and achieve the effects of high sensitivity and specificity, easy access, and minimally invasive access.

A technology for Hashimoto's thyroiditis and auxiliary diagnosis, applied in the field of genetic engineering, can solve the problems of rarely reported diagnostic effects, and achieve the effects of high sensitivity and specificity, easy access, and minimally invasive access.

CN106119396BActive Publication Date: 2020-02-21朱伟
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  • A plasma miRNA marker related to auxiliary diagnosis of Hashimoto's thyroiditis and its application
  • A plasma miRNA marker related to auxiliary diagnosis of Hashimoto's thyroiditis and its application
  • A plasma miRNA marker related to auxiliary diagnosis of Hashimoto's thyroiditis and its application

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Experimental program
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Embodiment Construction

[0033] From 2013 to 2014, the inventor collected a large number of venous plasma samples from patients with Hashimoto's thyroiditis and normal physical examination people from the First Affiliated Hospital of Nanjing Medical University, and selected 100 cases of Hashimoto's thyroiditis by sorting out the sample data And 100 normal control samples were used as the experimental samples for the initial screening of Exiqon miRNA qPCR panel chip and subsequent series of qRT-PCR verification. The selected patients' plasma samples were all from newly treated patients who were confirmed as Hashimoto's thyroiditis by endocrinologists based on the positive test results of peroxidase antibody (TPOAb) and antithyroglobulin antibody (TGAb). The demographic data and clinical data of these samples were collected systematically.

[0034] Refer to the flowchart ( figure 1 ), randomly selected 20 Hashimoto’s thyroiditis samples and 10 normal controls from the Hashimoto’s thyroiditis and normal...

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Abstract

The invention discloses a plasma miRNA marker related to hashimoto thyroiditis assisted diagnosis and application of the plasma miRNA marker. The marker is one or more of miR-205, miR-20a-3p, miR-375, miR-296, miR-451 and miR-500a. The plasma miRNA marker serving as a novel biological marker is good in stability, minimally invasive, easy to obtain and high in sensitivity and specificity. The development and utilization of the molecular marker can provide a new direction for the diagnosis and further treatment of various diseases including the hashimoto thyroiditis. The hashimoto thyroiditis plasma miRNA marker with clinical diagnosis potential can be obtained in a targeted manner by researches. The reliability and reproducibility of the group of miRNA serving as the non-invasive marker of the hashimoto thyroiditis are proved by researches.

Description

technical field [0001] The invention belongs to the field of genetic engineering and relates to a plasma miRNA marker related to auxiliary diagnosis of Hashimoto's thyroiditis and its application. Background technique [0002] Hashimoto's thyroiditis (HT), also known as chronic lymphocytic thyroiditis, is an organ-specific autoimmune disease that may cause glandular enlargement and even clinical or subclinical hypothyroidism. Recent evidence suggests that genetic susceptibility and environmental factors are major contributors to Hashimoto's thyroiditis, but the exact pathogenesis remains unclear. Early diagnosis and intervention may help prevent the development of HT and thyroid dysfunction. Currently, the diagnosis of HT is mainly based on invasive fine-needle aspiration biopsy. Autoantibodies in serum (ATAS) and ultrasonography are now available for the diagnosis of HT, but lack specificity. Most HT patients have no obvious symptoms in the early stage, so it is easy to ...

Claims

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Application Information

Patent Timeline
21 Feb 2020
Publication
CN106119396B
IPC
C12Q1/6883; C12N15/113
CPC
C12Q1/6883; C12Q2600/158; C12Q2600/178
Inventors
朱伟; 周鑫