Extraction, separation and purification process of brasenia schreberi polysaccharide

Abstract

The invention discloses an extraction, separation and purification process of brasenia schreberi polysaccharide. According to the process, the supercritical CO2 extraction technology is utilized, the defect that the extraction rate is low through a hot water extraction method and a NaOH extraction method is overcome, and the safety problem appearing in the situation that strong base needs to be used in NaOH extraction does not exist. When a crude product of the brasenia schreberi polysaccharide is purified, alkaline protease is adopted for treating and removing protein; cetyl trimethyl ammonium bromide and the brasenia schreberi polysaccharide can form quaternary amine complexes, the complexes do not dissolve in an aqueous solution with a low ionic strength and can dissociate and dissolve when the ionic strength is high, and the separation and purification effect is better; by using 312 weak-acid anionic exchange resin chromatography, some positively-charged impurities can be removed, and the separation and purification effect is better.

Description

Technical field [0001] The invention belongs to the technical field of methods for extracting polysaccharides from plants, and in particular relates to a process for extraction, separation and purification of Brasenia polysaccharides. Background technique [0002] Brasenia schreber JFGme1 (Brasenia schreber JFGme1) is a perennial freshwater aquatic herb of the primitive perianth subclass Nymphaeaceae Ulva, also known as horseshoe grass, lake vegetables, water anemone, and dew sunflower. It is native to southeast China and grows in ponds. , Lakes and swamps. Brasenia has rich nutritional value and medicinal value. According to the records of "Compendium of Materia Medica", Brasenia candidum has the functions of diminishing thirst and heat, curing jaundice, thickening the stomach and intestines, relieving scorching, removing water, and detoxifying. Studies have shown that Brasenia polysaccharide has anti-tumor, anti-oxidation, lowering blood fat, lowering blood sugar, anti-inflamm...

AI Technical Summary

Problems solved by technology

However, due to the high viscosity of Brasenia polysaccharide, it is difficult to filter, concentrate and purify, so the extraction rate of hot water extraction method and NaOH extraction method is low, and there is a safety problem in the use of strong alkali in NaOH extraction.

And these methods extraction yield is low, and utilization rate is low, has greatly restricted the development of water shield industry