Extraction, separation and purification process of brasenia schreberi polysaccharide

A technology for separation and purification of seaweed, applied in the field of extraction and separation and purification of seaweed polysaccharide, can solve the problems of high viscosity of seaweed polysaccharide, restricting the development of seaweed industry, low extraction yield, etc., and achieves good separation and purification effect and better separation and purification effect Effect

Active Publication Date: 2016-11-23
CHONGQING THREE GORGES MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high viscosity of Brasenia polysaccharide, it is difficult to filter, concentrate and purify, so the extraction rate of hot water extraction method and NaOH extraction method is low, and

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Extraction of crude Brasenia polysaccharides:

[0027] (1.1) Crush Brasenia serrata to 100-200 mesh, and put the smashed Brasenia serrata to supercritical CO 2 In the extraction kettle, seal the kettle, remove the air, and introduce the carrier agent to extract the extract. The extraction temperature is 45.2℃, the extraction pressure is 21.3MPa, the extraction carbon dioxide flow rate is 25L / h, the carrier agent is distilled water, and the dosage is 420ml / kg , Extract 4h;

[0028] (1.2) Add a mixture of chloroform and n-butanol to the extract and shake it well, centrifuge at 3000 r / min for 10 min, and take the supernatant I. The volume ratio of chloroform and n-butanol in the mixture is 4:1 , The volume ratio of the extraction liquid to the mixed liquid is 4:1;

[0029] (1.3) Adjust the supernatant liquid to pH 8.0 with NaOH, then add activated carbon to the supernatant liquid for decolorization and suction filtration to obtain the filtrate; further use distilled water to...

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PUM

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Abstract

The invention discloses an extraction, separation and purification process of brasenia schreberi polysaccharide. According to the process, the supercritical CO2 extraction technology is utilized, the defect that the extraction rate is low through a hot water extraction method and a NaOH extraction method is overcome, and the safety problem appearing in the situation that strong base needs to be used in NaOH extraction does not exist. When a crude product of the brasenia schreberi polysaccharide is purified, alkaline protease is adopted for treating and removing protein; cetyl trimethyl ammonium bromide and the brasenia schreberi polysaccharide can form quaternary amine complexes, the complexes do not dissolve in an aqueous solution with a low ionic strength and can dissociate and dissolve when the ionic strength is high, and the separation and purification effect is better; by using 312 weak-acid anionic exchange resin chromatography, some positively-charged impurities can be removed, and the separation and purification effect is better.

Description

Technical field [0001] The invention belongs to the technical field of methods for extracting polysaccharides from plants, and in particular relates to a process for extraction, separation and purification of Brasenia polysaccharides. Background technique [0002] Brasenia schreber JFGme1 (Brasenia schreber JFGme1) is a perennial freshwater aquatic herb of the primitive perianth subclass Nymphaeaceae Ulva, also known as horseshoe grass, lake vegetables, water anemone, and dew sunflower. It is native to southeast China and grows in ponds. , Lakes and swamps. Brasenia has rich nutritional value and medicinal value. According to the records of "Compendium of Materia Medica", Brasenia candidum has the functions of diminishing thirst and heat, curing jaundice, thickening the stomach and intestines, relieving scorching, removing water, and detoxifying. Studies have shown that Brasenia polysaccharide has anti-tumor, anti-oxidation, lowering blood fat, lowering blood sugar, anti-inflamm...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003Y02P20/54
Inventor 熊书孙厚良马强李国利李春雷饶清风胡艳玲李小山
Owner CHONGQING THREE GORGES MEDICAL COLLEGE
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