Method for extracting and separating asiaticoside by countercurrent extraction combined with membrane separation

Abstract

The invention discloses a method for extracting and separating asiaticoside by countercurrent extraction combined with membrane separation, characterized by comprising the following steps of: (1) adding asiatic centella hay to a countercurrent extractor, adding water, extracting, combining extracted solutions, and finely filtering to obtain the extracted solution of asiatic centella; (2) performing secondary membrane filtration on the extracted solution of asiatic centella, simultaneously removing moisture under the operating pressure of 0.05-0.20MPa to obtain an asiaticoside concentrated solution; and (3) performing vacuum microwave drying on the asiaticoside concentrated solution, and pulverizing to obtain asiaticoside powder. The invention has the advantages of having short extraction time, high separation rate, high degree of automation in operation, really safe products, low investment and simple filtering equipment, reducing the consumption of organic solvent, improving the purity, quality and yield of extract, and reducing the production cost.

Description

technical field [0001] The invention relates to the extraction of total asiaticosides, in particular to a method for countercurrent extraction combined with membrane separation, extraction and separation of total asiaticosides Background technique [0002] Centella asiatica (L.) Urban is the dry or rooted whole plant of the dicotyledonous plant Umbelliferae. Widely distributed in the south of the Yangtze River Basin, it is a commonly used Chinese herbal medicine in folk areas in Guangdong. In my country, Centella asiatica has been used externally and taken internally to treat diseases for more than 2,000 years. Centella asiatica is cold in nature, bitter in taste, and pungent, and has the effects of clearing heat and promoting dampness, promoting blood circulation to stop bleeding, detoxifying and reducing swelling. The whole plant mainly contains triterpenoids, polyacetylenes, volatile oils and other components. The main components of triterpenoids include Asiaticoside (M...

AI Technical Summary

Problems solved by technology

There are toxic substances such as toluene and xylene in the separation of macroporous resins. At the same time, the operation steps of using macroporous resins mainly include resin pretreatment, adsorption of liquid medicine, water washing, desorption, water washing, and resin regeneration. , the process is complicated and the operation is cumbersome; in addition, the pretreatment, desorption and regeneration of the macroporous adsorption resin require a large amount of ethanol and acid and alkali, which increases the cost of production and brings pressure on environmental protection

The 200910152717.7 patent application discloses the extraction method of total saponins of Centella asiatica and its application in the field of medicine. In the extraction and separation, organic solvents such as chloroform, ethyl acetate, and n-butanol are used for extraction. The solvent has certain toxicity and is volatile. The production process is difficult to control, the production cost is high, and the residue of organic solvent is the fatal flaw of this process

In addition, the 200810303151.9 patent application uses water or low-carbon alcohol thermal diafiltration, heat reflux and ultrasonic extraction; the 200910152717.7 patent application uses ethanol ultrasonic extraction; The extraction method requires a large amount of solvent and a long extraction time

Use ethanol as the extraction solvent to extract, and the ethanol needs to be recovered after extraction, which will cause the loss of alcohol, increase the energy consumption of production, and greatly increase the production cost

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