Method for distinguishing Chinese honeybee honey from Apis mellifera honey based on major royal jelly protein ingredients

A technology for the main protein of royal jelly and honey, which is applied in the direction of analyzing materials, conducting material analysis by electromagnetic means, measuring devices, etc., can solve the problems of consumer trust crisis, loss of bee product enterprises, and unfavorable development of the bee product market.

Inactive Publication Date: 2016-11-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the price of Chinese bee honey in the domestic market is 3-5 times that of Italian bee honey. Driven by profit, some unscrupulous traders use Italian bee honey to pretend to be or mix it with Chinese bee honey to earn h

Method used

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  • Method for distinguishing Chinese honeybee honey from Apis mellifera honey based on major royal jelly protein ingredients
  • Method for distinguishing Chinese honeybee honey from Apis mellifera honey based on major royal jelly protein ingredients
  • Method for distinguishing Chinese honeybee honey from Apis mellifera honey based on major royal jelly protein ingredients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for discriminating Chinese bee honey and Italian bee honey is realized through the following steps:

[0021] (1) Extraction of total protein in honey: Weigh 10g of Zhongfeng honey and Italian bee honey respectively, put each in a 50ml centrifuge tube, add 10ml of sterile water, shake and mix well, and centrifuge at 8000g / min at 4°C Centrifuge for 10 minutes to remove impurities such as beeswax and pollen. Carefully draw the supernatant into a new 50ml centrifuge tube with a pipette gun. Pre-cool the supernatant in a 4°C refrigerator for 10 minutes, then add it to a -20°C freezer for pre-cooling 20ml of absolute ethanol, stirred gently in an ice-water bath for 15 minutes, then centrifuged at a speed of 15000g / min for 10 minutes at 4°C, discarded the supernatant, added 1.5ml of sterile water to the precipitate, stirred with a glass rod, and then Shake and mix well, then divide into 1.5ml centrifuge tubes and store in -80°C refrigerator for later use. Honey from d...

Embodiment 2

[0030] The information of the honey samples in this example is shown in Table 1, and the samples were stored in a 4°C refrigerator for later use. The experiment was carried out according to the method steps of Example 1, wherein 1-4 in the sample number corresponded to channels 1-4 in the gel map, sample numbers 1-4 corresponded to channels 5-8 in the gel map, and the band results are shown in figure 1 , the results of mass spectrometry are shown in Table 2.

[0031] Table 1 Honey sample information table

[0032]

[0033]

[0034] The mass spectrometry results of the differential protein bands are as follows:

[0035] Table 2 Identification results of differential protein bands by mass spectrometry

[0036] stripe number

[0037] It can be seen from the gel map and mass spectrometry results that there are obvious differences in the protein composition of the two honeys, and the two can be identified according to the difference in the main protein of royal je...

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Abstract

The invention provides a method for distinguishing Chinese honeybee honey from apis mellifera honey based on major royal jelly protein ingredients. By means of a biological method and the difference between Chinese honeybee honey and apis mellifera honey in protein, different protein bands appear by conducting protein electrophoresis on a honey sample after the honey sample processed, protein categories are determined by conducting mass spectrometry on the different protein bands, and then the two kinds of honey from different bee species are identified. The problems that Chinese honeybee honey and apis mellifera honey available in market are hard to distinguish, and apis mellifera honey pretends to be Chinese honeybee honey or is mixed in Chinese honeybee honey are solved, and the situation that low-price apis mellifera honey pretends to be high-price Chinese honeybee honey can be inhibited. The method is used for distinguishing Chinese honeybee honey from apis mellifera honey.

Description

technical field [0001] The invention belongs to honey quality control methods, and relates to a method for separating and identifying the main protein components of royal jelly in honey, in particular to a method for identifying Chinese bee honey and Italian bee honey based on the main protein components of royal jelly, and applying it to Chinese bee honey In the identification of honey and Italian bee honey. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, mix with their own secretions, and fully brew. The chemical composition of honey is quite complex, and more than 180 substances have been identified in honey. The main components in honey are glucose and fructose, accounting for about 65%-80% of the total amount of honey, followed by water, accounting for 16%-25% %. In addition, honey also contains a variety of vitamins, proteins, and various minerals and trace elements necessary for hum...

Claims

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Application Information

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IPC IPC(8): G01N27/447G01N27/62
CPCG01N27/447G01N27/62
Inventor 胡福良张言政郑火青张翠平
Owner ZHEJIANG UNIV
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