Preparation method of novel conventional cultivar for crops
A technology of crops and conventional seeds, applied in the field of preparation of conventional crops of crops, can solve the problems of increasing the difficulty of intellectual property protection of new varieties, and the risk of seed production is small, so as to strengthen the protection of intellectual property rights, reduce infringements, and avoid illegal reproduction. The effect of stealing
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Embodiment 1
[0029] Using Huang Huazhan as a material for intellectual property protection of a new species, the genome editing technology mediated by CRISPR / Cas9 engineering nuclease was used to edit the temperature-sensitive gene TMS5 in the genome of Huang Huazhan (HHZ), and create a thermosensitive dual-purpose nuclear sterile Huang Huazhan S with the loss of TMS5 function. (HHZ-tms5). Utilizing the "dual pressure selection method of natural low temperature environment and artificial low temperature environment" (Yang Yuanzhu, et al. Breeding strategy and progress of light-temperature-sensitive dual-purpose nuclear sterile line of Yangtze River early indica rice. China Agricultural Science and Technology Herald, 2004, 6 (6) : 36-40) stress selection, and bred Huanghuazhan male sterile line (HHZ-tms5) with low starting point temperature for fertility conversion, stable fertility, good fertility and good outcrossing. Select a base with ecological advantages for breeding, and carry out la...
Embodiment 2
[0031] Using Huanghuazhan as the intellectual property protection material of the new variety, the two-line GMS strain 1S was used as the female parent to cross with Huanghuazhan (HHZ), and Huanghuazhan was used as the recurrent parent for continuous backcrossing for 5-8 generations and then self-crossed. In the process of backcrossing, combined with TMS5 gene-specific marker detection and genomic background analysis of genome-wide markers (such as SSR, SNP, etc.), select the single plant that contains the tms5 allele and has the closest genome background to Huanghuazhan and backcrosses with Huanghuazhan until 99% of the background is the same as that of Huang Huazhan, self-breeding and breeding, further using the "double pressure selection method of natural low temperature environment and artificial low temperature environment" (Yang Yuanzhu, et al. Breeding strategy for early indica rice photothermosensitive dual-purpose nuclear male sterile line and progress. China Agricultu...
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