Mouse model for mTORC1 (mechanistic target of rapamycin 1) pathways and method for building mouse model

A mouse model and pathway technology, applied in animal husbandry and other fields, can solve problems such as lack, and achieve the effect of simple method and good application prospects

Inactive Publication Date: 2016-12-07
SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES +1
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

β-cell-specific mTORC1 knockout mice are currently lacking

Method used

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  • Mouse model for mTORC1 (mechanistic target of rapamycin 1) pathways and method for building mouse model
  • Mouse model for mTORC1 (mechanistic target of rapamycin 1) pathways and method for building mouse model
  • Mouse model for mTORC1 (mechanistic target of rapamycin 1) pathways and method for building mouse model

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Experimental program
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Embodiment 1

[0022] 1. Mice Breeding and Mating Program

[0023] 8-week-old sexually mature male mice and female mice were caged together, and the mice were numbered and separated into cages when they were 3 weeks old. At the same time, a small amount of mouse tail tissue was cut for subsequent genotyping. On the C57 strain of Raptor lox / lox mice, cross with RIP-Cre mice to obtain Raptor heterozygous mice, namely RIP-Cre Raptor lox / w mice, and through self-mating of Raptor heterozygous mice , to obtain Raptor lox / lox mice with or without RIP-Cre. The former is to specifically knock out Raptor mice in β cells (βRapKO mice), and mice without RIP-Cre are used as control mice.

[0024] 2. Genotyping

[0025] 1) DNA extraction

[0026] a) Mince the tissue with clean and sterile scissors and add 100ul of AD1 buffer and 25ul of AD2 buffer;

[0027] b) After incubating at room temperature for 10 minutes, place in a metal bath at 95°C for 3 minutes;

[0028] c) Add 100ul of AD3 buffer, mix wel...

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Abstract

The invention relates to a mouse model for mTORC1 (mechanistic target of rapamycin 1) pathways and a method for building the mouse model. The mouse model is a mouse. Raptor is specifically knocked out of beta cells in the mouse. The method includes allowing Raptor lox / lox mice of C57 strains and RIP-Cre mice to carry out mating to obtain Raptor heterogeneous mice, namely RIP-Cre Raptor lox / w mice; allowing the Raptor heterogeneous mice to carry out mating to obtain Raptor lox / lox mice with RIP-Cre so as to obtain the mouse model. The mouse model and the method have the advantages that the method is simple; as discovered by means of comparison, the TORC1 signaling pathways in the mouse can be specifically inhibited after the Raptor is specifically knocked out by the beta cells in the mouse, accordingly, foundations can be laid for follow-up research, and the mouse model and the method have excellent application prospects.

Description

technical field [0001] The invention belongs to the field of type 2 diabetes, and particularly relates to a mouse model for mTORC1 pathway and a method for establishing the same. Background technique [0002] With economic development and changes in lifestyle, the incidence of type 2 diabetes is on the rise globally. It is estimated that in 2010, more than 13 million deaths were directly caused by diabetes or caused by macrovascular complications. In the same period, the prevalence of diabetes in China was as high as 11.6%, and only 10% of them got good blood sugar control through treatment. It can be seen that it is very necessary and urgent to strengthen the research on type 2 diabetes. It is generally believed that peripheral tissue insulin resistance and pancreatic β-cell dysfunction are the two central links in the pathogenesis of type 2 diabetes, and the latter is particularly critical for the maintenance of systemic glucose metabolism homeostasis. The mechanism resea...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 宁光汪启迪顾燕云倪启程张宏利聂爱芳
Owner SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES
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