Ultralow-temperature preservation method for grapefruit germplasm pollen
An ultra-low temperature preservation and pollen technology, which is applied in the fields of plant preservation, botanical equipment and methods, and application, can solve the problems of poor sealing and packaging of pollen, insufficient rewetting of recovered pollen, and increased damage to pollen cells by ice crystals. The effect of germination rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0049] Example 1 Effects of thawing methods and germination medium on pollen germination rate after cryopreservation
[0050] 1. Experimental materials
[0051] The materials used in the experiment were three pomelo germplasm pollen of "Zuoshi pomelo", "Kui pomelo" and "Beizhuan pomelo" provided by the National Fruit Tree Germplasm Citrus Garden (Chongqing).
[0052] 2. Experimental method and specific steps
[0053] - Pollen drying treatment: the collected anthers are dried under an incandescent lamp (at a temperature of about 30°C) until the water content is 10-20%.
[0054] —Preservation in sealed packaging with liquid nitrogen: After the pollen is properly dried, put it into a 1.8ml cryopreservation tube (Nunc, USA), and put it into liquid nitrogen (CBS S3000-AB SERIES) for storage.
[0055] — Thawing: Take the frozen storage tubes containing pollen out of the liquid nitrogen tank and thaw them in two ways: (1) rinse with tap water at 25°C for about 15 minutes; (2) quick...
Embodiment 2
[0061] Example 2 The effect of rehumidification on the germination rate of grapefruit pollen
[0062] 1. Experimental materials
[0063] The varieties used in the experiment were two pomelo germplasm pollen of "Zuoshiyou" and "Kuiyou" provided by the National Fruit Tree Germplasm Citrus Garden (Chongqing).
[0064] 2. Experimental method and specific steps
[0065] - Pollen drying treatment: the collected anthers are dried under an incandescent lamp (at a temperature of about 30°C) until the water content is 10-20%. Three samples of "left pomelo - water content 10%", "left pomelo - water content 20%" and "kui pomelo - water content 10%" were obtained.
[0066] —Preservation in sealed packaging with liquid nitrogen: After the pollen is properly dried, put it into a 1.8ml cryopreservation tube (Nunc, USA), and put it into liquid nitrogen (CBS S3000-AB SERIES) for storage.
[0067] — Thawing: Take the frozen storage tubes containing pollen out of the liquid nitrogen tank and t...
PUM

Abstract
Description
Claims
Application Information

- Generate Ideas
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com