Ultralow-temperature preservation method for grapefruit germplasm pollen
An ultra-low temperature preservation and pollen technology, which is applied in the fields of plant preservation, botanical equipment and methods, and application, can solve the problems of poor sealing and packaging of pollen, insufficient rewetting of recovered pollen, and increased damage to pollen cells by ice crystals. The effect of germination rate
Active Publication Date: 2016-12-07
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Abstract
The invention relates to a plant ultralow-temperature preservation method, and in particular to an ultralow-temperature preservation method for a grapefruit germplasm pollen. The ultralow-temperature preservation method comprises the following steps: (1) drying a pollen, namely, drying the pollen till the water content is 10-20%; (2) performing pollen cryopreservation, namely, sealing and packaging the dried pollen, and putting the pollen in liquid nitrogen for preservation; (3) performing pollen anabiosis, namely, taking out the pollen from the liquid nitrogen, and thawing the pollen for 5-15 minutes at 25-37 DEG C; (4) performing pollen rewetting for 0-3 hours; (5) performing in-vitro germination. By adopting the method, key factors of ultralow-temperature preservation applicable to the grapefruit germplasm pollen, such as the water content range, the thawing condition, the rewetting condition and the in-vitro germination condition, are specified. Due to optimization and restriction on the conditions, the germination rate of the grapefruit germplasm pollen after ultralow-temperature preservation is remarkably increased.
Application Domain
Dead plant preservation
Technology Topic
Water contentGermination +8
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