Industrialized production method for nano-gold antibacterial agent

Abstract

The invention provides an industrialized production method of nano-gold antibacterial agent. The method comprises the following steps: (1) mixing a chloroauric acid solution with a solution containing aminopyrimidines and amino small molecules or a potassium chloroplatinate solution , obtain a mixed solution; (2) quickly add a solution of reducing agent to the mixed solution under stirring, and obtain the nano-gold antibacterial agent after fully reacting; wherein, the volume of the nano-gold antibacterial agent is more than 5L, It is preferably 5 to 100 L, and the addition time of the reducing agent is not more than 5 minutes, preferably not more than 1 minute, and more preferably 10 to 60 seconds. Also provided are the nano-gold antibacterial agent produced by the method, and the application of the method or the nano-gold antibacterial agent.

Description

technical field [0001] The invention relates to a preparation method of a nano-gold antibacterial agent, in particular to an industrial production method of a nano-gold antibacterial agent and the nano-gold antibacterial agent prepared by the method. Background technique [0002] Silver nanoparticles (AgNPs) are widely used due to their excellent antibacterial properties, but their use in medical applications is limited due to their instability and high toxicity to human cells. In recent years, gold nanoparticles with better stability and less toxicity to human cells have been gradually reported as antibacterial agents. Nanogold (AuNPs) is a new type of antibacterial agent. Most of the research on the preparation of nanogold antibacterial agents is in the small-scale stage of the laboratory. The preparation process is more complicated and difficult to industrialize. not too good. The mercaptopyrimidine-modified nano-gold antibacterial agent reported in patent CN102188383A ...

AI Technical Summary

Problems solved by technology

Nanogold (AuNPs) is a new type of antibacterial agent. Most of the research on the preparation of nanogold antibacterial agents is in the small-scale stage of the laboratory. The preparation process is more complicated and difficult to industrialize. not too good

The mercaptopyrimidine-modified nano-gold antibacterial agent reported in patent CN102188383A has poor antibacterial effect on Gram-positive bacteria such as Staphylococcus aureus

Hong-Zheng Lai (Lai H Z, Chen W Y, Wu C Y, et al. Potent antibacterial nanoparticles for pathogenic bacteria [J]. ACS applied materials & interfaces, 2015, 7(3): 2046-2054.) reported vancomycin-modified gold nanoparticles Compared with vancomycin alone, antibacterial agents have greatly improved their antibacterial properties, but the antibiotic vancomycin present on gold nanoparticles still poses hidden dangers to humans and nature due to the problem of bacterial resistance.

Zhao yuyun et al. (Zhao Y, Jiang X. Multiple strategies to activate gold nanoparticles as antibiotics [J]. Nanoscale, 2013, 5(18): 8340-8350. and Zhao Y, Ye C, Liu W, et al. Tuning the composition of AuPt bimetallic nanoparticles forantibacterial application[J].Angewandte Chemie International Edition,2014,53(31):8127-8131.) reported aminopyrimidine / amino-containing small molecule two-component drug-modified nano-gold antibacterial agent and nano-gold Platinum bimetallic antibacterial agents have strong broad-spectrum antibacterial properties, and their toxicity to human cells is far lower than that of nanosilver, and they will not produce bacterial resistance. They are a class of very promising antibacterial agents, but their preparation Process reducing agent NaBH 4 It is added to the reaction solution in the form of dropwise addition, which will be very time-consuming and difficult to achieve in large-scale production. Secondly, after the reaction of nano-gold, it needs to be dialyzed with a dialysis bag, which is still difficult to achieve in industrial production.

Images