Preparation method and use of molybdenum oxide nanoparticles with targeted photothermal and photodynamic therapy functions

A technology of photodynamic therapy and nanoparticles, which is applied in the field of photodynamic therapy materials and photothermal, can solve the problem of molybdenum oxide nanoparticles not being targetedly modified, molybdenum oxide nanoparticles failing to reach cancer cells accurately, photothermal therapy Blindness and other problems, to achieve good photodynamic effect, good photothermal conversion efficiency and photothermal stability, good near-infrared absorption effect

Active Publication Date: 2019-12-27
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the synthesis of multifunctional molybdenum oxide nanoparticles has made great progress, but most of the synthesized molybdenum oxide nanoparticles have not been targetedly modified, which makes it impossible for the molybdenum oxide nanoparticles to reach accurately during the application process. The location of cancer cells, the process of photothermal therapy is blind
At the same time, the photodynamics of molybdenum oxide nanoparticles have not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Weigh 0.4g of hyaluronic acid, 0.48g of ammonium heptamolybdate, and 0.9g of glucose, add 15mL of water, and stir to dissolve completely into a transparent liquid;

[0028] 2) Add 4 drops of concentrated hydrochloric acid with a 3mL disposable dropper, heat the oil bath to 90°C, and react for 6 hours to obtain a dark blue solution;

[0029] 3) Add dehydrated ethanol, then centrifuge with a centrifuge at a centrifugal speed of 10000r / min, and centrifuge for 10min; the amount of dehydrated ethanol added is: the volume ratio of dehydrated ethanol to the blue solution is 2:1;

[0030] 4) Wash the solid obtained in step 3) several times with water, add absolute ethanol, and centrifuge to obtain a dark blue solid;

[0031] 5) Vacuum drying is used to obtain the hyaluronic acid-modified molybdenum oxide nanoparticles of the present invention.

Embodiment 2

[0033] 1) Weigh 0.4g of hyaluronic acid, 0.48g of ammonium heptamolybdate, and 0.9g of glucose, add 10mL of water, and stir to dissolve completely into a transparent liquid;

[0034] 2) Add 3 drops of concentrated hydrochloric acid with a 3 mL disposable dropper, heat the oil bath to 90°C, and react for 6 hours to obtain a dark blue solution;

[0035] 3) Add dehydrated ethanol, then centrifuge with a centrifuge at a centrifugal speed of 10000r / min, and centrifuge for 10min; the amount of dehydrated ethanol added is: the volume ratio of dehydrated ethanol to the blue solution is 2:1;

[0036] 4) Wash the solid obtained in 3) several times with water, add absolute ethanol, and centrifuge to obtain a dark blue solid;

[0037] 5) Vacuum drying is used to obtain the hyaluronic acid-modified molybdenum oxide nanoparticles of the present invention.

Embodiment 3

[0039] 1) Weigh 0.8g of hyaluronic acid, 0.96g of ammonium heptamolybdate, and 1.8g of glucose, add 15mL of water, and stir to dissolve completely into a transparent liquid;

[0040] 2) Add 4 drops of concentrated hydrochloric acid with a 3mL disposable dropper, heat the oil bath to 90°C, and react for 6 hours to obtain a dark blue solution;

[0041] 3) Add dehydrated ethanol, then centrifuge with a centrifuge at a centrifugal speed of 10000r / min, and centrifuge for 10min; the amount of dehydrated ethanol added is: the volume ratio of dehydrated ethanol to the blue solution is 2:1;

[0042] 4) Wash the solid obtained in 3) several times with water, add absolute ethanol, and centrifuge to obtain a dark blue solid;

[0043] 5) Vacuum drying is used to obtain the hyaluronic acid-modified molybdenum oxide nanoparticles of the present invention;

[0044] The products prepared in Examples 1, 2, and 3 can all be well dispersed in aqueous solution and can exist stably.

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PUM

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Abstract

The invention discloses a preparation method and application of molybdenum oxide nanoparticles used for targeted photo-thermal and photodynamic therapy. The nanoparticles are synthesized from hyaluronic acid, ammonium heptamolybdate and glucose through the one-step method. The method is easy and convenient to operate, and raw materials are low in price and easy to obtain. The prepared nanoparticles modified by hyaluronic acid have the advantages of being low in cell toxicity, good in biocompatibility and the like. Besides, the molybdenum oxide nanoparticles have good absorption in infrared and near-infrared areas, water solubility of the nanoparticles modified by hyaluronic acid is better, and photothermal conversion efficiency is good. The nanoparticles have photosensitization and can generate active oxygen under irradiation of infrared or near-infrared light. The prepared molybdenum oxide nanoparticles can be used for photo-thermal and photodynamic cancer treatment in a targeted mode, and have wide application prospects in the field of cancer treatment.

Description

technical field [0001] The invention relates to photothermal and photodynamic therapy materials, in particular to a preparation method and application of molybdenum oxide nanoparticles with targeted photothermal and photodynamic therapy functions. Background technique [0002] Photothermal therapy for tumors (Photothermal therapy, PTT) is a therapy that converts light energy into heat energy through photothermal conversion agents to bring tumor tissue to a certain temperature and kill cancer cells. Photodynamic therapy (Photodynamic therapy, PDT) is a new cancer treatment method in which photodynamic reagents generate reactive oxygen species under laser irradiation, thereby increasing the level of reactive oxygen species in tumor tissue, and then killing cancer cells. Compared with traditional surgical treatment, chemotherapy and radiotherapy, PTT and PDT have the advantages of minimal trauma, low toxicity, and repeatable treatment. Therefore, these two therapies are promis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61K41/00A61K9/51A61K47/61A61P35/00
CPCA61K9/5161A61K41/0052A61K41/0057
Inventor 蒋邦平王源源沈星灿周波韦方棉
Owner GUANGXI NORMAL UNIV
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