Activated sludge floc specific gravity measuring method based on spectrophotometry and cell separation

A technology of activated sludge and spectrophotometry, applied in the direction of color/spectral characteristic measurement, test sample preparation, etc., to achieve the effect of accurate measurement

Inactive Publication Date: 2016-12-07
QINGDAO TECHNOLOGICAL UNIVERSITY
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Problems solved by technology

[0004] However, the test and analysis of Percoll cell separation liquid for the specific gravity of activated sludge flocs have not been seen in the literature.

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  • Activated sludge floc specific gravity measuring method based on spectrophotometry and cell separation
  • Activated sludge floc specific gravity measuring method based on spectrophotometry and cell separation
  • Activated sludge floc specific gravity measuring method based on spectrophotometry and cell separation

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Embodiment Construction

[0030] The method for measuring the specific gravity of activated sludge flocs based on spectrophotometry and cell separation of the present invention includes a testing stage and a data processing stage.

[0031] 1. Testing phase

[0032] (1) Preparation of different specific gravity Percoll mixed solutions:

[0033] In order to achieve physiological osmotic pressure, Percoll reagent is mixed with 8.5% NaCl (NaCl solution of mass concentration 8.5%) with 9:1 volume ratio to make Percoll solution, then Percoll solution and physiological solution (0.85% NaCl or 0.15mPBS) were prepared according to the proportions in the table below.

[0034] Table 1 The preparation table of Percoll mixed solution with different specific gravity gradients

[0035]

[0036]

[0037]

[0038] (2) Preparation of different specific gravity gradient layers:

[0039] In order to accurately measure the specific gravity of activated sludge flocs, select adjacent 4-8 (this embodiment chooses ...

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Abstract

An activated sludge floc specific gravity measuring method based on spectrophotometry and cell separation includes a testing stage and a data processing stage. The testing stage includes the steps that firstly, Percoll mixed solutions with different specific gravities are prepared; secondly, different specific gravity gradient layers are prepared; thirdly, a sample is injected; fourthly, centrifugation is conducted; fifthly, sampling is conducted; sixthly, detection is conducted. Data processing includes the steps that the differences between the absorbancy values of Percoll and sludge floc mixed solutions and the absorbancy value of a blank sample are calculated, the sum of the differences of the absorbancy values is calculated, accordingly the proportion of the absorbancy differences, accounting for the sum, of the Percoll mixed solutions with different specific gravities is calculated, and finally, according to the specific gravities of the Percoll solutions with different gradients and the proportion accounted by the solutions, the final specific gravity of sludge floc is calculated by means of weighted average. The method is combined with the spectrophotometry measurement method, accurate measurement of the specific gravity of the activated sludge floc is achieved, and influences of the specific gravity of the activated sludge floc on activated sludge settleability are determined.

Description

technical field [0001] The invention relates to a method for measuring the specific gravity of activated sludge flocs, belonging to the technical field of sewage treatment. Background technique [0002] In the field of sewage biological treatment, although the activated sludge method has been widely used worldwide as a cost-effective sewage biological treatment technology, the parameter indicators reflecting the settlement performance of activated sludge are mainly qualitative and macro indicators, which objectively reflect Due to the lack of objective and accurate measurement methods for the index of activated sludge specific gravity, it is impossible to analyze the change of activated sludge specific gravity and determine its influence on the settlement performance of activated sludge in the actual operation and management of sewage treatment. [0003] In the field of pathological and biological research, Percoll density gradient centrifugation has been successfully applie...

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Application Information

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IPC IPC(8): G01N21/33G01N1/38G01N1/34
CPCG01N21/33G01N1/34G01N1/38
Inventor 毕学军刘振超程丽华刘长青李倩
Owner QINGDAO TECHNOLOGICAL UNIVERSITY
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