Sample culture carrier

A technology for culturing carriers and samples, which can be used in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve problems such as deficiencies, and achieve the effects of high accuracy, reasonable space layout, and convenient operation process.

Active Publication Date: 2016-12-14
南京乐诊生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, when conducting cell culture and comparison tests in the process, although ordinary culture devices can already provide the carrying space for th

Method used

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Examples

Experimental program
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Effect test

Example

[0043] Example 1: In the circumferential direction of the rotating tube, the drain groove, the equalizing hole, the tube feeding hole, and the cylinder feeding hole are arranged in a clockwise direction. During the operation of equalizing liquid control volume (that is, the aforementioned process of discharging liquid through the equalizing hole to make the liquid volume in each dish consistent), the liquid draining tank rotates clockwise with the rotating tube to align with the equalizing hole (the pipe feeding liquid during this process) The hole is not connected with the corresponding cylinder liquid supply hole), after the equalizing liquid control volume is completed, the drain tank continues to rotate clockwise and staggered with the equalizing hole. At this time, the pipe liquid supply hole and the corresponding cylinder liquid supply hole still have not reached the connected state. Then if you continue to rotate the tube clockwise, the tube feeding hole can be connected ...

Example

[0044] Example 2: In the circumferential direction of the rotating tube, the equalizing hole, draining groove, tube feeding hole, and cylinder feeding hole are arranged in a clockwise direction, and the amount of liquid is controlled (that is, the liquid is discharged through the equalizing hole to The process of making the amount of fluid in each dish consistent) During operation, the drain tank rotates counterclockwise with the rotating tube to align with the equalizing hole (the pipe feeding hole is not connected to the corresponding cylinder feeding hole in this process), and the equalizing liquid is controlled. After completion, the drain tank rotates clockwise and is staggered with the equalizing hole. At this time, the tube feeding hole and the corresponding cylinder feeding hole still have not reached the connected state, and then if you continue to rotate the tube clockwise, you can let the tube feed hole Connect with the corresponding cylinder to the liquid hole.

[004...

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PUM

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Abstract

The invention discloses a sample culture carrier. The sample culture carrier comprises a base and multiple culture vessels, all the culture vessels are stacked, each culture vessel comprises a vessel body, a vertical cylinder, a bump and an injection tube, the end face of the top of each vessel body is provided with a vessel top groove, the top of the base is provided with a base top groove, and the bump of the lowest culture vessel is located in the base top groove; a rotating tube penetrates through all the vertical cylinders, the rotating tube and the vertical cylinders can rotate relatively, the outer side wall of the rotating tube makes close contact with the inner side walls of the vertical cylinders, and a drain groove is formed in the outer side wall of the rotating tube; each vertical cylinder is provided with a liquid equalizing hole communicated with the interior of the corresponding vessel body, each liquid equalizing hole is provided with a cell filtering net and a liquid equalizing one-way valve, and the lower end of each injection tube is communicated with the interior of the corresponding vessel body; the bump of the upper culture vessel is located in the vessel top groove of the corresponding lower culture vessel. The sample culture carrier has the advantages of being suitable for cell culture, determination and comparison, reasonable in spatial arrangement, stable in structure, convenient and rapid to operate, good in consistency of adjustment in the experimental process and high in accuracy.

Description

technical field [0001] The invention belongs to the technical field of cell culture, in particular to a sample culture carrier. Background technique [0002] Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. The growth of cells requires a nutrient environment, and the nutrient matrix used to maintain cell growth is called a medium. The medium can be divided into liquid medium and solid medium according to its physical state. In addition to being used in production, cell culture technology is currently used more in experiments (such as determining the parameters of group samples, and conducting comparisons and research), and liquid culture media is commonly used in experiments. In addition, cell culture requires the use of cell culture devices, such as petri dishes, incubators, etc. Cell culture in general environments can directly use petri dishes, and if some special environments (such as power on, heating, etc...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/22
CPCC12M23/10C12M23/42C12M23/48C12M29/00
Inventor 王铁苗
Owner 南京乐诊生物技术有限公司
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