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Laying duck feather picking character important gene HTR2C molecular marker and application

A technology of molecular markers and laying ducks, which is applied in the direction of recombinant DNA technology, DNA/RNA fragments, microbial determination/inspection, etc., and can solve the problems of molecular marker mining and application reports, etc.

Inactive Publication Date: 2016-12-14
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been a large number of research reports on molecular markers and their application in breeding, but there are few reports on the mining and application of molecular markers related to behavioral traits, especially the research and application of molecular markers on feather pecking behavior of laying ducks is still a new field. field

Method used

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  • Laying duck feather picking character important gene HTR2C molecular marker and application
  • Laying duck feather picking character important gene HTR2C molecular marker and application

Examples

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Embodiment 1

[0022] The HTR2C molecular marker of an important gene for feather pecking traits of laying ducks of the present invention comprises the following steps:

[0023] 1. DNA extraction:

[0024] Use the magnetic bead method to extract DNA from the egg duck breast muscle sample to be tested, and its animal genome DNA extraction kit (provided by Bioengineering Company can be used). After the DNA was extracted, it was detected by 2% agarose gel electrophoresis, and its OD value and sample concentration were measured by an ultraviolet spectrophotometer, and stored in a -20°C refrigerator.

[0025] 2. Primer design:

[0026] Primers were designed using Primer Primer 5.0 and synthesized by Primer Biotechnology Co., Ltd. The primer sequence information is shown in Table 1:

[0027]

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PUM

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Abstract

The invention discloses a laying duck feather picking character important gene HTR2C molecular marker. The molecular marker comprises the following steps of 1, DNA extraction, wherein DNA is extracted from a laying duck pectoral muscle sample; 2, primer design, wherein Primer Primer 5.0 is applied for primer design; 3, PCR amplification, wherein a 20-microliter reaction system is applied, PCR amplification conditions are set for PCR amplification, and 5 microliters of a product is taken for detecting 2.0% agarose gel electrophoresis; 4, amplification product fragment purification, carrier connection, conversion and positive clone screening and sequencing; 5, SNP mutation site screening and association analysis, and determination of SNP related to feather picking characters. The method is applied for searching for the molecular marker related to feather picking characters so that feather picking caused by genetic factors can be eliminated. References can be provided for building the method for selecting molecules with behavior characters.

Description

technical field [0001] The invention belongs to a poultry molecular marker selection method, in particular to a molecular marker method for laying ducks. Background technique [0002] Behavior, as a complex quantitative trait, is regulated by multiple genes and affected by various environmental factors. Among these many behaviors, feather pecking behavior (non-nutritional deficiency) has always attracted people's attention, because it has become the main problem affecting animal welfare in large-scale intensive poultry farming in Europe, America and China. Feather and aggressive pecking. Aggressive pecking is defined as a rapid attack on the head, body and anus of the opponent. In severe cases, the poultry skin can be maimed or cannibalism. It has been reported abroad that the interleukin and serotonin receptor gene HTR2C in laying hens is related to feather pecking behavior and feather damage, but it has not been reported in laying ducks. [0003] Among the two authorized...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨胜林王达娜朱娜陆曼柳诚刚王旭平周美迪
Owner GUIZHOU UNIV
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