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A strain of Bacillus and its composite bacterial preparation for efficiently degrading nitrogen pollutants

A technology of bacillus and bacillus licheniformis, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problems of difficult decomposition of harmful substances and poisoning of farmed animals, and achieve great application potential, convenient storage and use, and wide application foreground effect

Active Publication Date: 2019-05-31
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to increase the growth rate of aquaculture animals, excessive feeding leads to the accumulation of a large amount of residual bait, and the organic nitrogen and other harmful substances dissolved in the residual bait are not easy to be decomposed. The accumulation of nitrogen in the water body exceeds the tolerance range of aquaculture organisms. Animals produce direct toxic effects

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  • A strain of Bacillus and its composite bacterial preparation for efficiently degrading nitrogen pollutants
  • A strain of Bacillus and its composite bacterial preparation for efficiently degrading nitrogen pollutants
  • A strain of Bacillus and its composite bacterial preparation for efficiently degrading nitrogen pollutants

Examples

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Embodiment 1

[0045] Example 1 The main characteristics of Bacillus albicans and its degradation experiment to water pollutants

[0046] 1. the main feature of described bacillus albino bacillus BBHS-01 (Bacillus baekryungensis) is as follows:

[0047] (1) Morphological characteristics:

[0048] The shape is rod-shaped, can move, can produce spores, reproduce by binary fission, and the bacteria can be single, paired or short-chained.

[0049] (2) Culture characteristics:

[0050] In seawater 2216E basal medium or prawn bait medium (commercially available prawn bait 20g, ground into fine powder, sterilized aged seawater 1000ml, soaked for 48h, centrifuged to take supernatant, added agar 25g, adjusted pH 7.8, 121°C, sterilized for 20min ) at (5-45)°C, the optimum growth temperature range is (18-28)°C, the optimum temperature range for the degradation of prawn bait components is (24-28)°C, and the growth pH range is 4 ~10, the optimum pH range for growth and bait degradation is 7.5~8.0, the...

Embodiment 2

[0070] Embodiment 2 Antagonism experiment of Bacillus albicans BBHS-01, Bacillus subtilis and Bacillus licheniformis

[0071] Antagonism experiment of three strains: Bacillus albicans BBHS-01, Bacillus subtilis and Bacillus licheniformis were streaked with 2216E solid medium, cultured at 28°C for 24 hours, and single colonies were picked on 2216E solid medium Two crosses were drawn in pairs, and the streaked plate was incubated at 28°C for 24 hours, and then the growth of the strains was checked. The results of the antagonism experiment showed that there was no antagonistic effect among the three strains.

Embodiment 3

[0072] The preparation method of embodiment 3 compound bacteria preparations

[0073] The preparation method of the composite bacteria preparation of the present embodiment may further comprise the steps:

[0074] (1) Activation and purification of strains: Inoculate Bacillus albicans BBHS-01, Bacillus subtilis and Bacillus licheniformis on the improved 2216E solid medium respectively, and cultivate them at 28°C for 22 hours; the improved 2216E The medium formula of the solid medium is as follows: peptone 6.0g, yeast powder 3.0g, aged sea water 1000mL, agar 25.0g and trace element solution 1ml, the trace element composition of the trace element solution is: 0.4g / L K 2 HPO 4 ·3H 2 O, 0.3g / L MgSO 4 ·7H 2 O, 0.5g / L MnSO 4 , 0.1g / L FeSO 4 ·7H 2 O, 0.2g / L CuSO 4 , adjust the pH to 7.6, sterilize at 121°C and 101KPa for 30 minutes; pick a single colony and inoculate it into 2216E solid slant medium, and purify and cultivate at 28°C for 20 hours. The obtained test tube slant ...

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Abstract

The invention belongs to the field of microbes and their applications, and in particular relates to a strain of bacillus that efficiently degrades nitrogen pollutants, and also relates to a composite bacterial preparation with the above-mentioned bacillus as the main component; The preparation method of compound bacteria preparation. The Bacillus BBHS-01 for efficiently degrading nitrogen pollutants of the present invention is Bacillus baekryungensis BBHS-01 (Bacillus baekryungensis), which was preserved in the General Microorganisms Preservation Center of China Microbiological Cultures Preservation Management Committee on December 7, 2012 , the deposit number is: CGMCC NO.6939. The advantages of the bacillus albino BBHS-01 of the present invention are that it has the effect of efficiently degrading nitrogen pollutants in aquaculture water, can purify water quality, and significantly improve the aquaculture water environment.

Description

technical field [0001] The patent of the present invention belongs to the field of microbes and their applications, and in particular relates to a strain of Bacillus that efficiently degrades nitrogen pollutants, which is Bacillus albicans BBHS-01, which can efficiently degrade nitrogen pollutants in aquaculture water and can be applied to the water environment of aquaculture Regulation and micro-ecological restoration can purify water quality and significantly improve the culture water environment; the present invention also relates to a composite bacterial preparation with the above-mentioned Bacillus albicans BBHS-01 as the main component; The invention discloses a preparation method of a composite bacteria preparation whose main component is bacillus BBHS-01. Background technique [0002] Since the 1980s, driven by the scarcity and limitation of wild resources and the demand of the vast consumer market, my country's aquaculture industry has developed rapidly, and the deg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/125C12R1/10C02F103/20
CPCC02F3/34C02F2103/20C12N1/20C12N1/205C12R2001/07
Inventor 田相利赵坤闫法军李步先
Owner OCEAN UNIV OF CHINA
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