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Disposable reaction liquid suction reactor

A technology of reactor and reaction solution, which is applied in the field of research equipment, can solve the problems of cumbersome process, time-consuming, unfavorable proteome, etc., and achieve the effect of easy observation and analysis

Active Publication Date: 2018-08-24
GUIZHOU JINJIU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Connecting the two for protein two-dimensional capillary electrophoresis is the best analysis method, but connecting two capillary columns requires professional operation, which is not universal, and is very unfavorable for proteome research
[0006] The inventor found in the research that the currently used protein analysis methods are mainly to separate and analyze the protein samples to obtain the one-dimensional information of the samples. To obtain more information, a combination of various analysis methods is required. For example, after separating the protein , to collect the protein, the collected protein is hydrolyzed by protease, mainly using specific protease, and the obtained hydrolyzate (mainly peptide fragments) is separated and analyzed, mainly using high performance liquid chromatography / mass spectrometry, capillary electrophoresis / Mass spectrometry, mass spectrometry analysis, such a process is cumbersome, and many processes cannot be automated, and it takes a long time

Method used

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Experimental program
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Effect test

Embodiment 1

[0031] see Figure 1-Figure 4 , Embodiment 1 of the present invention provides a disposable reaction liquid absorption reactor, including a chip holder 101, a first reaction membrane 103, a reactor body 104 and a chip 102 for detecting the reaction process.

[0032] The chip 102 is fixed on the bottom of the chip holder 101, the top wall of the first reaction film 103 contacts the bottom wall of the chip 102, the top wall of the reactor main body 104 contacts the bottom wall of the first reaction film 103, and the main body of the reactor is provided with The liquid inlet channel 110 and the liquid outlet channel 111, the liquid inlet channel 110 and the liquid outlet channel 111 run through the reactor body 104 vertically, the bottom of the liquid inlet channel 110 is used to contact the reaction liquid, and the first reaction membrane 103 is provided with a drainage The chamber 109 , the liquid inlet channel 110 and the liquid outlet channel 111 are all in communication with...

Embodiment 2

[0050] see Figure 5 and Figure 6 , Embodiment 2 of the present invention provides a disposable reaction solution absorption reactor, including a chip holder 101, a first reaction membrane 103, a reactor body 104, a grasping hole 107, a peristaltic pump and a chip 102 for detecting the reaction process .

[0051] The chip 102 is fixed on the bottom of the chip holder 101 , the top wall of the first reaction film 103 is in contact with the bottom wall of the chip 102 , and the top wall of the reactor body 104 is in contact with the bottom wall of the first reaction film 103 . The main body of the reactor is provided with a liquid inlet channel 110 and a liquid outlet channel 111, and both the liquid inlet channel 110 and the liquid outlet channel 111 vertically penetrate the reactor main body 104, and the bottom of the liquid inlet channel 110 is used for contacting with the reaction liquid. The reaction membrane 103 is provided with a drainage chamber 109 , and both the liq...

Embodiment 3

[0057] This embodiment provides a disposable reaction liquid absorption reactor, which is different from the disposable reaction liquid absorption reactor in Example 1 in that the setting of the feed channel and the discharge channel is different. For the rest of the content, refer to Example 1. Yes, no more details here.

[0058] Specifically, in this embodiment, the feed channel includes two parts, the first feed channel 112 and the second feed channel 113, wherein the first feed channel 112 is used for the operator to inject samples, while the second feed channel 113 is vertical set up and set side by side with the discharge channel 111, such as Figure 7 As shown, in this embodiment, the second feeding channel 113 is four channels parallel to each other, Figure 7 The arrows in represent the flow direction of the reaction solution. First, the reaction solution is added from the first feed channel 112, and then driven by the power provided at the end of the discharge chan...

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PUM

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Abstract

The invention provides a disposable reaction liquid suction reactor and belongs to the field of research equipment. The disposable reaction liquid suction reactor comprises a chip seat, a first reaction membrane, a reactor body and a chip for detecting reaction process, the chip is connected at the bottom of the chip seat, the top wall of the first reaction membrane contacts with the bottom wall of the chip, the top wall of the reactor body contacts with the bottom wall of the first reaction membrane, the reactor body is provided with a liquid incoming passage and a liquid outgoing passage which both vertically penetrate the reactor body, the bottom of the liquid incoming passage is used for contacting with reaction liquid, the first reaction membrane is provided with a drainage cavity, and both the liquid incoming passage and the liquid outgoing passage are communicated with the drainage cavity. The disposable reaction liquid suction reactor is stable in reaction and convenient in chip detection.

Description

technical field [0001] The invention relates to the field of research equipment, in particular to a disposable reaction liquid absorption reactor. Background technique [0002] The study of proteome is one of the basic tasks in the post-genome era. The development of proteomics research relies more on the development of analytical methods. Currently, the main technologies used for protein analysis are two-dimensional gel electrophoresis, computer image analysis, protein identification technology, high performance liquid chromatography, and capillary electrophoresis. The most widely used method in biology is two-dimensional gel electrophoresis. Although this method is relatively mature, the entire analysis process is cumbersome and time-consuming due to the inability to perform automated operations and analysis under high electric fields due to technical reasons. , and this method is not quantitatively accurate. [0003] Protein identification techniques mainly include amin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00
CPCG01N35/00G01N2035/00237
Inventor 王磊韩克兵周庭波郑世阳
Owner GUIZHOU JINJIU BIOTECH