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A kind of polypeptide foaming agent, its preparation method and application

A technology of foaming agent and polypeptide solution, applied in the field of polypeptide foaming agent, can solve the problems of inability to be degraded by the human body, affect environmental safety, strong skin irritation, etc., and achieve good decontamination ability, high tolerance and enhanced function. Effect

Active Publication Date: 2020-01-24
GUANGZHOU MACRO SOURCE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when this type of foaming agent is used in cosmetics, it has strong irritation to human skin, and is biologically toxic and cannot be degraded by the human body.
At the same time, this type of foaming agent will have a greater impact on the environment, because it is difficult to be degraded, and it will affect environmental safety

Method used

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  • A kind of polypeptide foaming agent, its preparation method and application
  • A kind of polypeptide foaming agent, its preparation method and application
  • A kind of polypeptide foaming agent, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation method of polypeptide foaming agent comprises the following steps: (1) Synthesis of polypeptide powder: chemically synthesize a polypeptide having an amino acid sequence of LEELAEQLEELAEQLEELAEQ (wherein, L is leucine, E is glutamic acid, and A is alanine , Q is glutamine), and the purity of the polypeptide can be above 80%. (2) Purification of the polypeptide: put the polypeptide mother solution (10mg / mL) in a 3.5KDa dialysis bag, and dialyze it for 24 hours in 25mM HEPES buffer at pH7.4. (3) Preparation of polypeptide foam: In 25mM HEPES buffer solution with pH7.4, 0.3mg / mL polypeptide foaming agent can form abundant foam, and this foam can exist for more than two minutes. By adding calcium chloride (0.1 mg / mL CaCl 2 , 0.3mg / mL polypeptide), the existence time of the foam can be increased to more than three minutes, but the delicate richness of the foam is reduced. By adding zinc chloride (0.1mg / mL ZnCl 2 , 0.3mg / mL polypeptide), will form a relativ...

Embodiment 2

[0053] The preparation method of polypeptide foaming agent comprises the following steps: (1) Synthesis of polypeptide powder: chemically synthesize a polypeptide with VEELLSAVEELLSAVEELLSA amino acid sequence (wherein, L is leucine, E is glutamic acid, and A is alanine , V is valine, S is serine), and the purity of the polypeptide can be above 80%. (2) Purification of the polypeptide: put the polypeptide mother solution (10mg / mL) in a 3.5KDa dialysis bag, and dialyze it for 24 hours in 25mM HEPES buffer at pH 7.4. (3) Preparation of polypeptide foam: in 25mM HEPES buffer solution with pH 7.4, 2.0mg / mL polypeptide foaming agent can form abundant foam, and this foam can exist for more than two minutes. By adding calcium chloride (0.1 mg / mL CaCl 2 , 2.0mg / mL polypeptide), the existence time of the foam can be increased to more than three minutes, but the delicate richness of the foam is reduced. By adding zinc chloride (0.1mg / mL ZnCl 2 , 2.0mg / mL polypeptide), a relatively fi...

Embodiment 3

[0055] The preparation method of polypeptide foaming agent comprises the following steps: (1) Synthesis of polypeptide powder: chemically synthesize a polypeptide with an amino acid sequence of LEELAEQVEELLSALEELAEQ (wherein, L is leucine, E is glutamic acid, and A is alanine , Q is glutamine, V is valine, S is serine), and the purity of the polypeptide can be above 80%. (2) Purification of the polypeptide: put the polypeptide mother solution (10mg / mL) in a 3.5KDa dialysis bag, and dialyze it for 24 hours in 25mM HEPES buffer at pH 7.4. (3) Preparation of polypeptide foam: In 25mM HEPES buffer solution with pH 7.4, 5.0mg / mL polypeptide foaming agent can form abundant foam, and this foam can exist for more than two minutes. By adding calcium chloride (0.1 mg / mL CaCl 2 , 5.0mg / mL polypeptide), the existence time of the foam can be increased to more than three minutes, but the delicate richness of the foam is reduced. By adding zinc chloride (0.1mg / mL ZnCl 2 , 5.0mg / mL polypep...

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Abstract

The invention discloses a polypeptide foaming agent. The polypeptide foaming agent comprises a polypeptide; an amino acid sequence of the polypeptide is composed of 2 to 7 repeated amino acid sequences a or is composed of 2 to 7 repeated amino acid sequences b or is composed of a combination of the amino acid sequences a and the amino acid sequences b in any form, and the total number of combined amino acids is not more than 49, wherein each amino acid sequence a is LEELAEQ and each amino acid sequence b is VEELLSA; L is leucine, E is glutamic acid, A is alanine, Q is glutamine, V is valine and S is serine. The invention further discloses a preparation method and application of the polypeptide foaming agent. The polypeptide foaming agent provided by the invention has good foaming performance and rich and stable foams, has good dirt removing capability, has relatively high tolerance on hard water and electrolyte, and can be circularly regenerated; the polypeptide foaming agent can be degraded by a human body and has no pollution to the environment.

Description

technical field [0001] The invention belongs to the field of cleaning products, and in particular relates to a polypeptide foaming agent, its preparation method and application. Background technique [0002] The foaming agent is a kind of surface active substance, which mainly reduces the interfacial tension on the air-water interface, promotes the formation of small air bubbles in the slurry, expands the sorting interface, and ensures that the bubbles rise to form a foam layer. Foaming agents are generally surfactants, and their molecular structure is composed of non-polar lipophilic (hydrophobic) groups and polar hydrophilic (oleophobic) groups, forming both hydrophilic and lipophilic foams. The so-called "amphipathic structure" molecule. When the foaming agent is added to water, the hydrophilic group inserts into the water phase and the lipophilic group inserts into the oil phase or stands upright in the air, forming a directional arrangement on the interface layer or su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00C07K7/08C07K1/34A61K8/64A61Q5/02A61Q19/10
CPCA61K8/64A61Q5/02A61Q19/10C07K7/08C07K14/001
Inventor 张龙吴延恒
Owner GUANGZHOU MACRO SOURCE BIOTECH CO LTD