Microfluidic chip for capturing circulating tumor cell clusters and preparing method thereof

Abstract

The invention relates to a microfluidic chip for capturing circulating tumor cell clusters. A design template is made from negative photoresist, a triangular matrix is arranged on the design template, the thickness of the design template is 50-100 microns, and the distance between triangles is 10-20 microns. The triangles are equilateral triangles, the side length of the triangles is 92 microns, and the distance between the triangles is 12 microns. The design template is made from SU8 photoresist. The thickness of the negative photoresist is 50 microns and is uniform, and non-uniform size is avoided. The invention further discloses a preparing method of the microfluidic chip.

Description

technical field [0001] The invention belongs to the field of detection and separation of rare cells, and in particular relates to a microfluidic chip for capturing circulating tumor cell clusters and a preparation method thereof. The microfluidic chip is designed to capture tumor cell clusters in whole blood by using a special self-structure and gaps . Background technique [0002] Circulating tumor cells (CTCs) are tumor cells that come off from the primary tumor or metastases and enter the peripheral blood circulation. They have important clinical significance but are very rare. Circulating tumor cells exist in peripheral blood in different forms, including free single CTCs and aggregated CTMs (Circulating Tumor Microemboli). [0003] Studies have shown that CTM has a stronger metastatic potential than a single CTC, and is more closely related to tumor prognosis, and tumor cells with higher purity can be obtained from CTM. Compared with single CTCs, some tumor cells in C...

AI Technical Summary

Problems solved by technology

[0019] (1) Red blood cell lysate may destroy CTM

[0020] (2) Using EpCAM antibody for positive separation, CTM may not express EpCAM, resulting in failure to collect

[0022] (4) Using size separation, traditional filter pore membranes, such as ISET products, are easy to damage the CTM due to the high pressure at both ends of the filter pore

[0023] In short, the existing separation methods have disadvantages such as low efficiency, inability to maintain activity, etc.

For the isolation of circulating tumor cluster cells in blood, the capture of circulating tumor cluster cells is very inefficient in the case of limited blood samples

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