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A kind of method utilizing inulin to prepare fructan and gluconic acid

A technology of gluconic acid and fructan, applied in the field of separation and purification, can solve the problems of poor flavor, strong volatility and low product purity, and achieve the effects of high sweetness and solubility, mild separation conditions and high separation efficiency

Active Publication Date: 2019-07-30
HUNAN XINLI BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: 1. Activated carbon decolorization is non-selective, and part of the inulin is also removed during decolorization, which affects the yield of inulin; Drying requires a lot of energy; ③The high molecular impurities such as protein are not removed, the purity of the product is not high, and the flavor is not good; ④The inulin end group glucose is not removed, glucose is a high-calorie carbon source required by the human body, which is not good for diabetics and affect the flavor of inulin
The disadvantages of this method are: ① dissolve inulin with organic solvent dimethyl sulfoxide or dimethyl formamide
Due to the low boiling point of organic solvents, low recovery rate, serious loss of solvents and often easy to burn and explode, the production environment must be highly explosion-proof; in addition, many organic solvents have irritation or toxic side effects on the human body, and operators must be strictly protected; dimethyl sulfoxide and Dimethylformamide has a bitter taste or a special odor, which affects the flavor of high fructo-oligosaccharides; ② Use absolute ethanol as the second solvent to separate high-fructo-oligosaccharides and fructo-oligosaccharides
Because absolute ethanol consumption is big, volatility is strong, is difficult to reclaim fully, therefore, production cost is higher; 3. do not reclaim the fructo-oligosaccharide in the supernatant, wasted the valuable material that can be used as food additive and supplementary agent, increased production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of sucrase hydrolysis solution

[0032] First pump pure water into the stainless steel enzyme hydrolysis reaction kettle, and then dissolve the inulin in the pure water under stirring according to the ratio of inulin mass to pure water volume ratio (kg / L) of 1:10, and then start the hydrolysis The heating device of the reaction kettle is to heat the aqueous solution of dissolving inulin to 40°C and keep it warm for 10min, add invertase according to the ratio of invertase to inulin mass (kg / kg) at a ratio of 1:150, and then add the invertase at a temperature of 40°C 1. Treating for 10 hours under the condition that the stirring speed is 60r / min, a sucrase hydrolysis solution is prepared, which is used for preparing a glucose oxidase treatment solution in the next step.

[0033] (2) Preparation of glucose oxidase treatment solution

[0034] After step (1) is completed, heat up the invertase hydrolysis solution prepared in step (1) to 50°C and keep it warm...

Embodiment 2

[0046] (1) Preparation of sucrase hydrolysis solution

[0047] First pump pure water into the stainless steel enzyme hydrolysis reaction kettle, and then dissolve the inulin in the pure water under stirring according to the ratio of inulin mass to pure water volume ratio (kg / L) of 1:15, and then start the hydrolysis The heating device of the reaction kettle is used to heat up the aqueous solution of dissolving inulin to 42°C and keep it warm for 12min, add invertase according to the ratio of invertase to inulin mass (kg / kg) at a ratio of 1:225, and then add the invertase at a temperature of 42°C 1. Treating for 13 hours under the condition of a stirring speed of 80r / min, a sucrase hydrolysis solution is prepared, which is used in the next step to prepare a glucose oxidase treatment solution.

[0048] (2) Preparation of glucose oxidase treatment solution

[0049] After step (1) is completed, heat up the sucrase hydrolysis solution prepared in step (1) to 55°C and keep it warm ...

Embodiment 3

[0061] (1) Preparation of sucrase hydrolysis solution

[0062] First pump pure water into the stainless steel enzyme hydrolysis reaction kettle, and then dissolve the inulin in the pure water under stirring according to the ratio of inulin mass to pure water volume ratio (kg / L) of 1:20, and then start the hydrolysis The heating device of the reaction kettle is to heat the aqueous solution of dissolving inulin to 45°C and keep it warm for 15min. 1. Treating for 16 hours under the condition of a stirring speed of 100r / min, a sucrase hydrolysis solution is prepared, which is used in the next step to prepare a glucose oxidase treatment solution.

[0063] (2) Preparation of glucose oxidase treatment solution

[0064] After step (1) is completed, heat up the invertase hydrolysis solution prepared in step (1) to 60°C and keep it warm for 15 minutes, then add Glucose oxidase, and then treated at a temperature of 60° C. and a stirring speed of 100 r / min for 2 hours to prepare a gluco...

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Abstract

The invention discloses a method for preparing fructan and gluconic acid by using inulin and belongs to the technical field of separation and purification. The method comprises the following steps: by taking commercially available inulin as a raw material, preparing a sucrase hydrating solution; preparing glucose oxidase treating fluid; preparing an ultrafiltration separating solution; preparing a gluconic acid separating solution; preparing a gluconic acid concentrated solution; preparing a fructan separating solution; preparing fructo-oligosaccharide powder and polyfructose powder, thus obtaining two fructan powder and gluconic acid solution with the mass percent concentration of 10 to 15 percent. According to the technical scheme provided by the invention, a product with high added value is produced by the operations of enzymatic hydrolysis, separation and concentration; the product is high in purity and definite in application; the quality of the product meets the requirement of market; used production equipment is industrial equipment, namely separating devices such as a nanofiltration device, an ultrafiltration device, a reverse osmosis device and an electrodialysis device; the production equipment is mild in separation condition and high in separation efficiency; the adopted raw materials and solvent can be completely utilized, and no three wastes are discharged; the method is a classical green technology and facilitates popularization and application.

Description

[0001] 1. Technical field [0002] The invention belongs to the technical field of separation and purification, and in particular relates to a method for preparing fructan and gluconic acid from inulin. [0003] 2. Background technology [0004] Inulin is a class of carbohydrates with a degree of polymerization of 2-60, dehydration polymerization of fructose units through β-(2,1) glycosidic bonds and termination of glucose units. Inulin is an ideal functional food ingredient, and it is also a good raw material for the production of fructooligosaccharides, high fructose syrup, crystalline fructose and other products. In addition, inulin also has the functions of controlling blood lipids, lowering blood sugar, promoting mineral absorption, increasing intestinal bifidobacteria, preventing constipation and treating obesity, so it is widely used in health care products, dietary supplements for the elderly and other fields. Demand The amount is huge. [0005] Existing methods for p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/12C12P19/04C12P7/58
CPCC12P7/58C12P19/04C12P19/12C12P19/14
Inventor 余红梅袁德宽熊洋周洋剑晏艳姚萍宿长琼周小华杨洋
Owner HUNAN XINLI BIOLOGICAL SCI & TECH
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