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Alpha-glucosidase activity inhibitory component in Yeyanghe and its preparation method and application

A technology for glucosidase activity inhibition, which is applied in the field of α-glucosidase activity inhibition components and its preparation, can solve the problems of insufficient development and utilization, and achieve the effect of less impurities, easy realization and high purity

Active Publication Date: 2019-07-05
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yeyanghe is rich in resources, but has not been fully developed and utilized for a long time

Method used

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  • Alpha-glucosidase activity inhibitory component in Yeyanghe and its preparation method and application
  • Alpha-glucosidase activity inhibitory component in Yeyanghe and its preparation method and application
  • Alpha-glucosidase activity inhibitory component in Yeyanghe and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0033] The fresh wild yanghe is crushed to a particle size of 420 μm, and the methanol-water solution is used as the extractant, wherein the volume percentage of methanol in the methanol-water solution is 90%. According to the first solid-liquid ratio of 20g:500mL, the extractant is mixed with the pulverized Yeyanghe to obtain the extract. Under the condition of 20°C and normal pressure, the extract was stirred and extracted with a magnetic stirrer for 50 hours to obtain the crude extract of Yeyanghe. The crude extract was centrifugally filtered, the supernatant was collected and separated, and the residue was repeatedly extracted twice according to the above-mentioned extraction method, and the supernatants obtained three times were combined, and the supernatant was placed in a water bath at 45°C, Rotary evaporation at a rotation speed of 50 rpm and a vacuum of 0.07 MPa to obtain a crude extract extract, the weight of which is 10% of that of Yeyanghe raw material. The wet me...

Embodiment 2

[0035] Remove impurities and wash fresh wild yanghe, dry it at 20°C, and crush it to a particle size of 580 μm. Use absolute ethanol-water solution as the extractant, wherein the volume percentage of absolute ethanol in absolute ethanol-water solution is 70 %. According to the first material-to-liquid ratio of 100g: 6000mL, the extractant is mixed with the pulverized Yeyanghe to obtain the extract. Under the condition of 30°C and normal pressure, the extract was stirred and extracted with a magnetic stirrer for 20 hours to obtain the crude extract of Yeyanghe. The crude extract was subjected to centrifugal filtration, the supernatant was collected and separated, and the residue was repeatedly extracted twice according to the above-mentioned extraction method, and the supernatants obtained respectively for three times were combined, and the supernatant was placed in a water bath at 55°C for 30 Rotary evaporation at a rotation speed of 1 / min and a vacuum of 0.08 MPa to obtain a...

Embodiment 3

[0037] Remove impurities and wash fresh wild yanghe, dry it at 45°C, and crush it to a particle size of 500 μm. Use absolute ethanol-water solution as the extractant, wherein the volume percentage of absolute ethanol in absolute ethanol-water solution is 95 %. According to the first material-to-liquid ratio of 60g: 3200mL, the extractant is mixed with the pulverized Yeyanghe to obtain the extract. Under the condition of 25°C and normal pressure, the extract was stirred and extracted with a magnetic stirrer for 35 hours to obtain the crude extract of Yeyanghe. The crude extract was subjected to centrifugal filtration, the supernatant was collected and separated, and the residue was repeatedly extracted twice according to the above-mentioned extraction method, and the supernatants obtained three times were combined, and the supernatant was placed in a water bath at 50°C, 40 Rotary evaporation at a rotation speed of 1 / min and a vacuum of 0.09 MPa to obtain a crude extract extrac...

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Abstract

The invention relates to an alpha-glucosidase activity inhibition component in habenaria ciliolaris kranzl, and a preparation method and application of the alpha-glucosidase activity inhibition component, belonging to the fields of chemistry and chemical engineering. The preparation method comprises the following steps: crushing the habenaria ciliolaris kranzl until the particle size of the crushed habenaria ciliolaris kranzl is 420-580mu m; mixing an extracting agent and the crushed habenaria ciliolaris kranzl according to a first solid-liquid ratio of (20-100g): (500-6000mL), and extracting for 20-50h to obtain a crude habenaria ciliolaris kranzl extract; filtering, collecting supernatant, and concentrating to obtain extractum of the crude extract; dispersing the extractum of the crude extract into water according to a second solid-liquid ratio of 1g: (1-6mL), carrying out gradient elution on a sample solution in a macroporous adsorption resin chromatography column, and collecting and concentrating methyl alcohol elution peak with activity to obtain the activity inhibition component. The method is simple and easy to operate. The alpha-glucosidase activity inhibition component prepared by the method has stronger alpha-glucosidase inhibition activity, and has a better inhibiting effect especially for the activity of maltase.

Description

technical field [0001] The invention relates to the field of chemistry and chemical engineering, and in particular to an α-glucosidase activity inhibiting component in Yeyanghe and its preparation method and application. Background technique [0002] Diabetes mellitus (DM) is a disease of endocrine and metabolic disorders caused by insufficient insulin content in human blood. It is characterized by increased blood sugar and urine sugar, which can lead to damage to multiple systems and organs. Leading cause of cerebrovascular disease, blindness, amputations and renal failure. A variety of hypoglycemic drugs have been developed clinically, such as insulin secretion promoters that improve glucose tolerance and insulin response levels, agents that improve glucose metabolism, aldose reductase inhibitors that prevent and delay the occurrence of symptoms, and insulin sensitizers. Among them, α-glucosidase inhibitors have become a hot spot in the development and research of oral hy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/898A61P3/10
CPCA61K36/898A61K2236/333A61K2236/55
Inventor 高鸿苟安娜钟凯黄毅娜
Owner SICHUAN UNIV
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