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Identification and analysis method of urinary metabolic markers of coronary heart disease

An analysis method and coronary heart disease technology, applied in the field of identification and analysis of urine metabolic markers, can solve the problems of early detection, high cost, and low efficiency of detection of coronary heart disease

Active Publication Date: 2019-02-01
王喜军
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems of low efficiency, high cost and incapable of early detection and early treatment of existing methods for detecting coronary heart disease, and proposes a method for identifying and analyzing urine metabolic markers of coronary heart disease

Method used

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  • Identification and analysis method of urinary metabolic markers of coronary heart disease
  • Identification and analysis method of urinary metabolic markers of coronary heart disease
  • Identification and analysis method of urinary metabolic markers of coronary heart disease

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Experimental program
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specific Embodiment approach 1

[0020] Embodiment 1: The identification and analysis method of urinary metabolic markers of coronary heart disease comprises the following steps:

[0021] Step 1: collection and pretreatment of urine samples;

[0022] Step 2: preparation of urine sample;

[0023] Step 3: Separating the urine sample prepared in Step 2 by UPLC;

[0024] Step 4: The urine sample separated by UPLC in step 3 is directly injected into the mass spectrometer without splitting for positive and negative ion scanning analysis;

[0025] Step 5: Identify urine metabolic biomarkers according to Step 4, and obtain 39 potential urine biomarkers;

[0026] Step 6: Perform metabolic pathway analysis on the 39 potential urine biomarkers obtained in Step 5, and obtain 20 metabolic pathways;

[0027] Step 7: Use ROC curve analysis to further determine the biomarkers related to coronary heart disease according to the size of AUC.

specific Embodiment approach 2

[0028] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the specific process of the collection and pretreatment of the urine sample in the step one is:

[0029] The subjects’ first fasting morning urine and the last urine before going to bed at night were collected every day for 3 consecutive days; the urine was centrifuged at 12000-13000rpm at 4°C for 8-10min, and the supernatant was taken and stored in a -20°C refrigerator for later use ; Before the samples are frozen, pipette 1ml from each sample for aliquots, and keep the rest frozen and sealed as a whole.

[0030] Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0031] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the specific process of the preparation of the urine sample in the step two is:

[0032] The urine thawed at room temperature was centrifuged at 12,000-13,000 rpm and 4°C for 8-10 minutes, and the supernatant was taken, diluted with distilled water, shaken for 1 minute, and filtered through a 0.22 μm filter membrane.

[0033] Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides an identification and analysis method for coronary heart disease urine metabolic markers, and relates to an identification and analysis method for urine metabolic markers. The identification and analysis method for the coronary heart disease urine metabolites can be used for solving the problems of low efficiency, high expenses and incapability of realizing early detection and early treatment of an existing coronary heart disease detection method. The identification and analysis method comprises the following steps: 1, collecting and preprocessing a urine sample; 2, preparing the urine sample; 3, performing UPLC separation; 4, directly injecting the urine sample subjected to UPLC separation into a mass spectrometer without shunting, and performing cationic and anionic scanning analysis on the urine sample subjected to UPLC separation; 5, identifying urine biomarkers, thereby obtaining 39 potential urine biomarkers; 6, performing metabolism pathway analysis on the 39 potient urine biomarkers, thereby obtaining 19 metabolic pathways; and 7, further determining biomarkers related to the coronary heart disease according to the size of AUC by use of ROC curve analysis. The identification and analysis method is applied to the field of identification on metabolic markers.

Description

technical field [0001] The invention relates to a method for identification and analysis of urine metabolic markers. Background technique [0002] Coronary heart disease has become one of the main diseases affecting human health with a very high fatality rate and disability rate, accounting for 40% of all fatal events. And the number of deaths from cardiovascular diseases worldwide is increasing year by year. According to statistics, the number of deaths from cardiovascular diseases in developed countries will increase from 5 million in 2000 to 60 million in 2030. With the rapid development of economy in our country, people's living standards have been greatly improved, residents' lifestyles have changed, and the morbidity and mortality of cardiovascular diseases have also increased year by year. According to the survey results of "China Cardiovascular Disease Report 2013", the prevalence of cardiovascular disease among urban and rural residents in China is on the rise, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张爱华王喜军赵琪琪刘畅安娜
Owner 王喜军
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