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A method for identification of metabolic markers based on coronary heart disease with heart-yang deficiency syndrome

An identification method and technology for heart-yang deficiency, applied in the field of identification of metabolic markers, can solve the problems of insufficient sensitivity, difficult to effectively reveal the relationship between components and curative effects, and achieve good promotion value, low cost, and pain relief.

Active Publication Date: 2020-10-13
王喜军
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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the clinical diagnosis of coronary heart disease with heart-yang deficiency is still not sensitive enough, which makes it difficult to effectively reveal the relationship between the ingredients in the prescription and the curative effect, and proposes a metabolic marker based on coronary heart disease with heart-yang deficiency identification method

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  • A method for identification of metabolic markers based on coronary heart disease with heart-yang deficiency syndrome
  • A method for identification of metabolic markers based on coronary heart disease with heart-yang deficiency syndrome
  • A method for identification of metabolic markers based on coronary heart disease with heart-yang deficiency syndrome

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specific Embodiment approach 1

[0022] Specific embodiment one: a method for identifying metabolic markers based on coronary heart disease and heart-yang deficiency in this embodiment is specifically prepared according to the following steps:

[0023] Step 1. Using the UPLC-G2-Si-HDMS method to perform UPLC analysis on the serum samples of n groups of healthy subjects and n groups of subjects with coronary heart disease and heart-yang deficiency syndrome to determine the endogenous metabolites in the serum samples;

[0024] Step 2. Use the G2-Si-HDMS method to perform a full scan of endogenous metabolites in positive and negative ion modes to obtain a mass spectrometry metabolic profile; perform pattern recognition and statistical analysis based on the mass spectrometric metabolic profile, and select retention time and mass-to-core ratio Consistent data for identification and determination of endogenous metabolite structures;

[0025] Step 3: Identification of blood metabolic biomarkers in subjects with coro...

specific Embodiment approach 2

[0030] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, the UPLC-G2-Si-HDMS method is used to test the serum of n groups of healthy subjects and n groups of coronary heart disease subjects with heart-yang deficiency syndrome Samples were analyzed by UPLC to determine the specific process of endogenous metabolites in serum samples as follows:

[0031] The preparation method of blood samples, because the existence of endogenous proteins in blood is likely to cause the decline of the column system, so the purpose of blood sample preparation is mainly to deduct the interference of serum proteins, so that endogenous metabolites can be retained as much as possible, which is convenient for revealing blood The real situation of the metabolome; referring to previous studies, the organic solvent extraction method is the most suitable sample preparation method for plasma metabolomics. : The preparation method of blood samples, bec...

specific Embodiment approach 3

[0032] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the UPLC analysis chromatographic conditions: chromatograph: Waters AcquityTM UPLC liquid chromatograph (Waters Science and Technology Co., Ltd., the United States); Chromatographic column: ACQUITY UPLC BEH C18 Column (100mm×2.1mm i.d., 1.7μm) (Waters Group, USA); mobile phase: mobile phase A is 0.1% formic acid acetonitrile, mobile phase B is 0.1% formic acid water; column temperature Preset: 50°C; Preset sample chamber temperature: 4°C; Flow rate: 0.5ml / min; Table 1. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention discloses a method for identifying metabolic markers based on heart Yang deficiency syndrome of coronary heart diseases. The method solves the problem that the existing clinical diagnosis method of heart Yang deficiency syndrome of coronary heart diseases has poor sensitivity so that the relationship between ingredients in the formula and curative effects is difficult to recover. Themethod comprises 1, carrying out UPLC analysis on n healthy subjects and n patient subjects suffering from heart Yang deficiency syndrome of coronary heart diseases through a UPLC-G2-Si-HDMS method to determine endogenous metabolites in serum samples, 2, carrying out full scanning on the endogenous metabolites in a positive and negative ion mode through a G2-Si-HDMS method to determine endogenousmetabolite structures and 3, identifying blood metabolic biomarkers of subjects suffering from heart Yang deficiency syndrome of coronary heart diseases. The method is used in the field of metabolicmarker identification.

Description

technical field [0001] The invention relates to a method for identifying metabolic markers, in particular to an identification method for metabolic markers based on coronary heart disease and heart-yang deficiency. Background technique [0002] Coronary atherosclerotic heart disease (referred to as coronary heart disease, CHD) is one of the common cardiovascular diseases in clinical practice. The etiology and pathogenesis of coronary heart disease can be briefly summarized as deficiency of heart-yang and endogenous phlegm and blood stasis. Insufficient heart yang and weak blood circulation lead to stagnation of blood circulation and blockage of heart vessels; weakness of heart yang causes yin-cold evil qi to take over the yang position; yin evil is most likely to block the chest yang and cause coronary heart disease. Through the clinical observation of 118 patients with coronary heart disease, Cao Hongxin et al. found that the syndrome of heart-yang deficiency was 62 cases,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72
Inventor 张爱华曹洪欣宗文静王喜军
Owner 王喜军
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