Preparation method of enteric-coated dual immunopotentiator for veterinary use

An immune enhancer and enteric-coated technology, which is applied in the field of biopharmaceuticals, can solve the problems of inability to locate and release active ingredients, reduce the bioavailability of drugs, and have irritation, so as to achieve no bactericidal function, enhance the body's immunity, and improve The effect of production performance

Active Publication Date: 2019-11-22
HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In addition, most of the existing veterinary dual immunostimulants are irritating due to their medicinal properties. At the same time, the preparations are often ineffective due to the action of gastric juice or enzymes. The active ingredients of the drug cannot be released in a targeted manner in the gastrointestinal tract, which not only reduces the The bioavailability of the drug and the waste of the drug

Method used

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  • Preparation method of enteric-coated dual immunopotentiator for veterinary use
  • Preparation method of enteric-coated dual immunopotentiator for veterinary use
  • Preparation method of enteric-coated dual immunopotentiator for veterinary use

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Experimental program
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Effect test

Embodiment 1

[0035] A preparation method of an enteric-coated dual immunopotentiator for veterinary use, comprising the following steps:

[0036] Step 1) preparation of antimicrobial peptide:

[0037] 1.1 Isolation of white blood cells from pig blood

[0038] Gently shake the Erlenmeyer flask, filter the mixed blood with multiple layers of sterile gauze to filter impurities into a sterile beaker, centrifuge the filtered blood at 4°C 4000rpm / min for 10min, at this time, the suspension in the centrifuge tube can be seen to be divided into 3 layers , the upper layer is pale yellow plasma, the bottom layer is red blood cells, and there is a gray white white blood cell layer on the red blood cell layer. Harvest the white blood cells in the middle layer, transfer them to another clean test tube, add PBS solution, mix gently, 4 ℃ 2000rpm / Centrifuge for 20 min, discard the supernatant, repeat twice, take a small amount of precipitate for microscopic observation, and confirm that it is a white bl...

Embodiment 2

[0059] 4. Effect of enteric-coated veterinary dual immunopotentiator on animal production performance

[0060] 4.1 Test material

[0061] The enteric-coated veterinary dual immunopotentiator produced according to the method in Example 1.

[0062] Test time and place: from August 10 to October 23, 2015, in a chicken farm in Mengzhou City.

[0063] Experimental animals and management: All broilers have the same daily management and the same environmental conditions.

[0064] 4.2 Experimental design

[0065] The experimental grouping scheme design is shown in the table below:

[0066] Table 1 Design of experimental grouping scheme

[0067]

[0068]

[0069] This experiment adopted a single-factor completely randomized experimental design. 120 7-day-old healthy broilers were divided into 4 treatment groups on average, and each treatment group was set up with 3 repetitions, with 10 chickens in each repetition. Among them, on the basis of the conventional diet, each test ...

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Abstract

The invention belongs to the technical field of biological pharmacy and particularly relates to a preparation method of an enteric-coated livestock bivalent immunoenhancer with the functions of enhancing the immunity of piglets and broilers and resisting and killing bacteria. The preparation method comprises the following steps: separating leukocytes from pig blood, performing coarse extraction of antibacterial peptide from the separated leukocytes by breaking, and purifying the antibacterial peptide through gel filtration chromatography; performing anticoagulant centrifugation to obtain blood plasma; performing salting out and purification to obtain immune globulin; mixing the antibacterial peptide and immune globulin dry powder to obtain microcapsules; and enabling the microcapsules to pass enteric coating liquid to form an enteric coating slow-release layer on the surface of the microcapsule particles to obtain enteric-coated pellets. In the invention, the IgG content in animal serum can be remarkably increased, the piglet immunity is enhanced, the morbidity is reduced, and the animal productivity is improved; and moreover, the problem that the drug efficacy and bioavailability are weakened by a large quantity of peptidohydrolase and proteolytic enzyme in gastrointestinal tract is solved, and thus the drug absorption effect is better.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a preparation method of an enteric-coated veterinary dual immunopotentiator capable of enhancing animal body immunity, antibacterial and bactericidal effects. Background technique [0002] my country is the largest breeding country and the largest consumer of animal health products. However, compared with developed countries, the veterinary immune enhancer industry is backward. The development and industrialization of stable, efficient, residue-free, and pollution-free new immune enhancers are in line with The development trend of green food and green production is of great significance to healthy animal husbandry. [0003] In view of the current status of veterinary immune enhancers in my country, the project team isolated and extracted small molecule antimicrobial peptides from neutrophils of pig blood discarded in slaughterhouses, separated and purified i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/395A61K9/51A61K47/36A61P37/04A61K38/02
CPCA61K9/0002A61K9/5036A61K38/02A61K39/395A61K2300/00
Inventor 王永芬马辉张震郭宏伟郑鸣赵绪永
Owner HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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