Microalga culture method

A technology of microalgae and culture system, applied in the field of bioengineering, can solve the problems of low production efficiency, large area and restricting the development of microalgae industry.

Active Publication Date: 2017-02-22
GUOTOU BIO TECH INVESTMENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the microalgae cultivation techniques are focused on the photoautotrophic system, which has the disadvantages of low production efficiency, large floor area, and high harvesting cost, which seriously restricts the development of the microalgae industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1 Control 1 (Control-1)

[0071] Both the basal medium and the feed medium use ammonium salt (ammonium chloride) as the nitrogen source, and the carbon to nitrogen ratio in the basal medium and the feed medium remains unchanged. The initial glucose concentration is 5g / L. Feeding is carried out in a staged constant rate mode, the glucose concentration is controlled at 2-5g / L during the entire culture process, and the pH is constant (6.0) with 3M NaOH during the culture process.

Embodiment 2

[0072] Example 2 Control 2 (Control-2)

[0073] Both the basal medium and the feed medium use urea as the nitrogen source, and the carbon to nitrogen ratio in the basal medium and the feed medium remains unchanged. The initial glucose concentration is 5g / L. Feeding is carried out in a staged constant rate mode, the glucose concentration is controlled at 2-5g / L during the entire culture process, and the pH is adjusted to be constant at 6.0 with 1M HCl during the culture process.

Embodiment 3

[0074] Example 3 Experimental group 1 (Experiment-1)

[0075] The basic medium uses ammonium chloride as a nitrogen source, and there is no nitrogen source in the feed medium. During the cultivation process, ammonia water is used to adjust the pH to 6.0 and serve as a supplementary nitrogen source. The initial glucose concentration is 5g / L. Feeding is carried out in a staged constant rate mode, and the glucose concentration is controlled at 2-5g / L during the entire cultivation process.

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Abstract

The invention belongs to the field of microalga culture and relates to a heterotrophic culture method of microalgae such as scenedesmus. The method is characterized in that ammonium salt is used as the basic nitrogen source in a heterotrophic culture medium, and ammonia water is used as the pH regulator and/or supplementary nitrogen source. By the method, the biomass of the microalgae such as the scenedesmus can be increased effectively.

Description

Technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for heterotrophic culture of microalgae, in which ammonium salt is used as a basic nitrogen source, ammonia water is used as a pH regulator and / or a nitrogen source is used to effectively increase the biomass of microalgae. Background technique [0002] Because microalgae are rich in protein, lipids, polysaccharides, vitamins, antioxidants and other functional nutrients, they have a wide range of applications in the fields of pharmaceutical raw materials, health foods and bioenergy. At present, most of the microalgae culture technology focuses on the photoautotrophic system, which has the disadvantages of low production efficiency, large area, and high harvest cost, which severely restricts the development of the microalgae industry. Increasing the biomass of microalgae through artificial large-scale cultivation technology is the key to the development and utilization of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 金虎韩丹翔李坤朋侯国立徐全陈剑平胡强
Owner GUOTOU BIO TECH INVESTMENT CO LTD
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