A method for running a sequencer test

Abstract

The invention discloses a method for an operational test of a sequencer. The method comprise the steps that sequence testing libraries are constructed by adopting at least four connectors, wherein the four connectors contain different index sequences, and the last one basic group of the index sequences are A, G, C and T respectively; by adopting sequence testing primers corresponding to the four connectors, 3' tail end of the sequence testing primers is hybridized on the last but one basic group of the index sequences after the sequence testing primers hybridize with the libraries constructed by the connectors; when a sequence test in conducted, the four sequence testing libraries are sampled to different flow channels, the four sequence testing primers join the flow channels containing corresponding libraries respectively, conducting a first sequence testing circle, wherein each flow channel emits A, G, C OR T corresponding light with single color respectively, and a test of four basic group signal strength is thus achieved. According to the method for the operational test of a sequencer, the test of corresponding signal strength of the four basic groups can be achieved; the method can be further used for conducting a preliminary test on laser, detecting whether filter discs on four channels is accurate or not, and detecting whether a platform moving system and an automatic focusing system are normal or not. A simple, effective and comprehensive detecting method is provided for the operational test of the sequencer.

Description

technical field [0001] The present application relates to the field of gene sequencing, in particular to a method for a sequencer to run a test. Background technique [0002] The basic principle of next-generation sequencing based on the acquisition of optical signals is that the four bases of ATCG are marked with four different fluorescent dyes, and are excited to emit light of different wavelengths under different wavelengths of laser light. The light is transmitted to the CCD, and the fluorescent signal is collected by the CCD. [0003] Before a sequencer can be used normally, it usually needs to be tested in operation. The content of the sequencing operation test includes measuring the intensity of the signal corresponding to each base, so it is necessary that the entire chip emits a kind of fluorescence after the same sequencing cycle. In Illumina's patent CN101415839A, the method of cleavage site is used to make the "sequencing while synthesizing" reaction The first...

AI Technical Summary

Problems solved by technology

[0004] However, this method is only applicable to clustered arrays, and it can only guarantee that the first base incorporated in the sequencing reaction is always T, and it does not mention the other three bases ACG; that is, although each The first base of the cluster is all T, which can be used to show the intensity of each cluster itself, but the intensity of the signal corresponding to the other three bases cannot be determined

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