A method for simultaneously extracting polysaccharides and flavonoids from Phellinus Phellinus cultivation waste

A technology of cultivation waste and Phellinus japonica, which is applied in the direction of medical formula, plant raw materials, medical raw materials derived from fungi, etc., can solve the problems of polluting the environment and endangering Phellinus huang production, achieve great economic value, high yield, and simplify production The effect of craft

Active Publication Date: 2019-04-12
淳安千岛湖桑都食用菌专业合作社
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, if the postpartum cultivation waste is discarded in the environment, a large number of organisms harmful to Phellinus production will be produced, such as various molds, mosquitoes, mites, etc., and the environment will also be polluted

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for simultaneously extracting polysaccharides and flavonoids from Phellinus Phellinus cultivation waste, specifically comprising the following steps:

[0031] Step (1): drying and pulverizing the Phellinus Phellinus cultivation waste;

[0032] Step (2): Mix Phellinus japonica cultivation waste dry powder with alkaline ethanol solution (ethanol with a volume fraction of 80%, adjust pH=10 with sodium hydroxide) at a mass ratio of 1:10;

[0033] Step (3): ultrasonically treat the mixture in step (2) for 20 minutes (100 Hz, 1000W), and filter to obtain filtrate I and residue;

[0034] Step (4): Add deionized water 8 times the weight of the residue to the residue in step (3), mix well, adjust the pH value to 4, add a compound enzyme accounting for 0.2% of the weight of the mixture (the compound enzyme is cellulase : Glucose oxidase = 2:1 mixture, the same below), enzymatic hydrolysis at 45°C for 40 minutes, centrifugation of the enzymatic solution (6000 rpm, 10 min...

Embodiment 2

[0038] A method for simultaneously extracting polysaccharides and flavonoids from Phellinus Phellinus cultivation waste, specifically comprising the following steps:

[0039] Step (1): drying and pulverizing the Phellinus Phellinus cultivation waste;

[0040] Step (2): Mix Phellinus Phellinus cultivation waste dry powder with alkaline ethanol solution (volume fraction 85% ethanol, pH=11) at a mass ratio of 1:20;

[0041] Step (3): ultrasonically treat the mixture in step (2) for 30 minutes (200 Hz, 800 W), and filter to obtain filtrate I and residue;

[0042] Step (4): Add deionized water 10 times the weight of the residue to the residue in step (3), mix well, adjust the pH value to 4.5, add a compound enzyme accounting for 0.3% of the weight of the mixture, and enzymatically hydrolyze at 45°C for 50 minutes , the enzymatic hydrolyzate was centrifuged (6000 rpm, 10 minutes), and supernatant II and precipitate were separated;

[0043] Step (5): Filtrate I in step (3), remove ...

Embodiment 3

[0046] A method for simultaneously extracting polysaccharides and flavonoids from Phellinus Phellinus cultivation waste, specifically comprising the following steps:

[0047] Step (1): drying and pulverizing the Phellinus Phellinus cultivation waste;

[0048] Step (2): Mix Phellinus Phellinus cultivation waste dry powder with alkaline ethanol solution (90% ethanol by volume, pH=12) at a mass ratio of 1:30;

[0049] Step (3): ultrasonically treat the mixture in step (2) for 30 minutes (400 Hz, 200 W), and filter to obtain filtrate I and residue;

[0050] Step (4): Add deionized water 12 times the weight of the residue to the residue in step (3), mix well, adjust the pH value to 5.0, and add a compound enzyme accounting for 0.4% of the weight of the mixture (the compound enzyme is cellulase: Glucose oxidase = 2.5:1 mixture), enzymolysis at 50°C for 60 minutes, centrifugation of the enzymolysis solution (6000 rpm, 10 minutes), and separation of supernatant II and precipitate;

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a waste treatment method, in particular to a method for simultaneously extracting polysaccharide and flavone from phellinus igniarius sporophore cultivation waste. The method includes the steps: drying the phellinus igniarius sporophore cultivation waste, crushing the dried phellinus igniarius sporophore cultivation waste to obtain dry phellinus igniarius sporophore cultivation waste powder, and mixing the day powder and alkaline ethanol solution; ultrasonically treating the mixed solution for 20-40 minutes, and filtering to obtain filter solution and residues; adding deionized water into the residues, uniformly mixing, adjusting a pH (potential of hydrogen) value to be 4-6 to obtain mixed solution, adding compound enzyme, performing enzymolysis at the temperature ranging from 45 DEG C to 55 DEG C, and centrifuging the enzymolysis solution to obtain supernatant solution and sediments; rotatably evaporating filter solution to remove ethyl alcohol, adding ethyl acetate, extracting, separating solution, and performing pressure reduction, concentration and drying for upper layer solution to obtain phellinus igniarius sporophore flavone; performing pressure reduction and concentration for the obtained supernatant solution by the aid of a rotary evaporator, and performing alcohol precipitation and centrifugation to obtain supernatant solution and sediments. The sediments are phellinus igniarius sporophore polysaccharide.

Description

technical field [0001] The invention relates to a waste treatment method, in particular to a method for simultaneously extracting polysaccharides and flavonoids from Phellinus Phellinus cultivation waste. Background technique [0002] Phellinus mainly parasitizes on the trunks of mulberry, poplar, burgundy, pine, birch, etc. It is actually a general term for a class of medicinal fungi. As a traditional Chinese medicinal material in my country, Phellinus Phellinus was first recorded in "Shen Nong Baicao Jing" and Li Shizhen's "Compendium of Materia Medica". Phellinus polysaccharides, flavonoids, coumarin, ergosterol, larch acid, fatty acids, triterpenoids, aromatic acids and various amino acids, as well as xylose oxidase, urease, esterase, catalase , sucrase, lactase, cellulase and other enzymes. According to research statistics, Phellinus has more than 20 pharmacological functions, including antibacterial, anti-inflammatory, anti-oxidation, anti-tumor, enhancing body immun...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/07C08B37/00
CPCA61K36/07A61K2236/17A61K2236/19A61K2236/331A61K2236/333A61K2236/53A61K2236/55C08B37/0003
Inventor 王建功蔡为明金群力张作法方锦棋王星晨
Owner 淳安千岛湖桑都食用菌专业合作社
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap