Preparation method of anti-microbial populus tomentosa leaf general flavones

Abstract

The invention discloses a preparation method of anti-microbial populus tomentosa leaf general flavone. The preparation method of the anti-microbial populus tomentosa leaf general flavone comprises the following steps: firstly, weighing 3kg of populus tomentosa leaves, adding 70% alcohol, carrying out reflux extraction for three times, wherein the solid-liquid ratio is 1 to 10, mixing the extracts, and carrying out drying under reduced pressure, thus obtaining a crude extract; then, dissolving the crude extract in 1500ml of distilled water, carrying out adsorption by adopting 101 macroporous adsorption resin, and carrying out water elution till the solution is colourless; then, carrying out elution by adopting 40% alcohol, collecting the eluate, carrying out vacuum concentration till no alcohol smell exists, and making the constant volume, namely, 2000ml; and finally, enabling the solution to slowly pass through a polyamide chromatographic column, carrying out washing by adopting distilled water till the solution is colourless, carrying out elution by adopting 90% alcohol, collecting the eluate, and carrying out drying by distillation under reduced pressure, thus obtaining the populus tomentosa leaf general flavone with the purity being 98%. According to the preparation method, the flavone part enrichment is carried out by adopting the 101 macroporous adsorption resin in combination with the polyamide chromatography technology, the obtained sample is high in purity, and the method is simple and practical.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to a preparation method of antibacterial populus tomentosa leaf total flavonoids. Background technique [0002] Populus tomentosa is a kind of folk herbal medicine, which is used in the folk and clinical treatment of osteomyelitis with severe decubitus, and has a significant effect. Clinical studies have shown that bedsores and osteomyelitis can cause serious infections, and Staphylococcus aureus is the main bacterial species that induces infections. A large number of studies have shown that flavonoids have extensive antibacterial effects, and the leaves of Populus tomentosa are rich in flavonoids, which may be the main material basis for exerting antibacterial effects. Contents of the invention [0003] The purpose of the present invention is to provide a preparation method of anti-Staphylococcus aureus total flavonoids from Populus tomentosa leaves, aiming to solve...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a preparation method of anti-Staphylococcus aureus total flavonoids of Populus tomentosa leaves, aiming to solve the problems of unclear antibacterial efficacy parts of Populus tomentosa leaves and uncontrollable quality of crude extracts for treating diseases

Images