Preparation method and application of aptamer-modified chitosan nano fibers
A chitosan nanometer, nucleic acid aptamer technology, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve the problems of poor selectivity and low nucleic acid aptamer immobilization rate. , to achieve the effect of high affinity, stable loading effect and wide application range
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[0031] The present invention provides a kind of preparation method of nucleic acid aptamer modified chitosan nanofiber, in order to enable those skilled in the art to better understand the technical scheme of the present invention, the present invention will be further described below in conjunction with accompanying drawing and preferred embodiment detailed instructions.
[0032] This embodiment takes zearalenone as the target analysis object, and takes the preparation of zearalenone nucleic acid aptamer-modified chitosan nanofibers as an example, but this does not limit the protection scope of the present invention.
[0033] Such as figure 1 Shown, the preparation method of zearalenone nucleic acid aptamer modified chitosan nanofiber comprises the following steps:
[0034] (1) preparation of chitosan solution: take by weighing 0.1g of chitosan powder, add 10mL of distilled water, then add 60 μL of acetic acid, after stirring and dissolving, settle in a 500mL volumetric flas...
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