Preparation method and application of aptamer-modified chitosan nano fibers

Abstract

The invention discloses a preparation method and application of aptamer-modified chitosan nano fibers. The preparation method comprises the following steps: (1) preparing a chitosan solution; (2) preparing chitosan nano fibers; (3) chemical immobilization: taking the aptamer solution, adding an EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide) solution for activation, adding the chitosan nano fibers obtained in the step (2), and oscillating to react at normal temperature; and (4) after-treatment: carrying out solid-liquid separation on the reaction mixed solution obtained in the step (3) to obtain the aptamer-modified chitosan nano fibers. The aptamer-modified chitosan nano fibers are suitable for high-selectivity separation and enrichment of trace or ultra-trace mycotoxin substances and other substances in the food and environment, and have the advantages of high selectivity, high extraction capacity, high stability and simple preparation process.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and testing, and relates to dispersed solid-phase microextraction technology, in particular to a method for preparing a nucleic acid aptamer-modified chitosan nanofiber adsorbent. Highly selective separation and enrichment of components such as trace mycotoxins. Background technique [0002] Due to the limitations of the state of the components to be measured, the interference of coexisting components, or the low sensitivity of the determination method, most chemical detection and analysis methods require effective and reasonable pretreatment of the sample in advance to achieve enrichment and purification. Effect. However, compared with the rapid development of instrumental analysis technology, the progress of sample pretreatment technology is relatively slow. Currently commonly used sample pretreatment techniques such as liquid-liquid extraction and column chromatography generally hav...

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Problems solved by technology

[0007] Aiming at the problems of poor selectivity and low immobilization rate of nucleic acid aptamers in the existing solid phase extraction technology, the present invention combines the characteristics of nucleic acid aptamers with high affinity, strong specificity, and good compatibility with biological samples with chitosan nanofibers Combining the advantages of large specific surface area and easy chemical modification, a nucleic acid aptamer-modified chitosan nanofiber was developed for the separation and enrichment of trace or ultra-trace components in complex samples. Liquid chromatography, which can realize high selectivity and high sensitivity analysis and detection

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