Handheld SPR detector and bio-sample detection method

Abstract

The invention discloses a handheld SPR detector and a bio-sample detection method. The handheld SPR detector comprises a shell, a light incident component, a light receiving component and a light reflecting component for receiving light emitted by the light incident component and reflecting the same to the light receiving component are arranged in the shell, the light reflecting component comprises a reflecting prism and a sensing chip positioned on a reflecting face of the reflecting prism, the sensing chip comprises a substrate and a plated glass sheet, and a sample adding groove, a negative pressure chamber and a sample passage for communicating the sample adding groove with the negative pressure chamber. The light incident component, the light receiving component and the light reflecting component are fixed in the shell, so that wavelength, angle and intensity of incident light can be fixed; designing turnplates for large commercial SPP instruments on the market is avoided, so that the handheld SPR detector is simpler in integral structure, easy to operate and small in size.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a hand-held SPR detector and a method for detecting biological samples. Background technique [0002] Surface plasmon resonance (SPR) is a physical optical phenomenon. At the interface of two transparent media with different refractive index (such as glass and water), when a beam of light is incident from a medium with a high refractive index to a medium with a low refractive index, the light will be refracted and reflected. When the incident angle increases to a certain value, the refraction angle is equal to 90°, and the light is emitted along the direction tangent to the interface. The incident angle at this time is called the critical angle. If the incident angle exceeds the critical angle, the incident light will not enter another medium, but all will be reflected back into the incident medium, and total internal reflection will occur. [0003] In fact, althou...

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Problems solved by technology

[0005] Existing commercialized SPR biosensing systems are all large-scale laboratory instruments, with bulky, complex structures, strict requirements on the surrounding environment, and high prices, which greatly restrict the wide application of SPR sensing systems.

At present, most of the SPR instruments built by domestic laboratories adopt the method of angle scanning, and fix the prism and photoelectric detection device on the large and small turntables respectively, and are driven by two stepping motors. It improves the portability of the instrument; there are also some instruments that use wavelength modulation and use spectrometers for data collection and analysis. Spectrometers are expensive, which greatly increases the cost

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